Análisis in silico del gen subolesina como posible vacuna contra garrapatas Rhipicephalus microplus

Rodolfo Esteban Lagunes Quintanilla

Abstract


El objetivo del estudio fue utilizar estrategias bioinformáticas para predecir propiedades de interés del gen subolesina, así como realizar construcciones filogenéticas para identificar la relación que existe entre los aislados geográficos de garrapatas reportados en el mundo. El gen subolesina presenta 98 % de identidad entre los diferentes aislados de garrapatas R. microplus, mientras que, entre diferentes géneros (Ixodes spp., Amblyomma spp., Hyalomma spp.) fue de 77 %. Se detectaron 4 epítopos lineales de células B conservados en diferentes especies de garrapatas. El análisis de la estructura secundaria de la proteína Subolesina mostró dos α-hélices, tres regiones inespecíficas (coil) y ninguna β plegada. De los epítopos predichos, será útil demostrar su capacidad inmunoprotectora contra cepas de garrapatas R. microplus de diferentes aislados geográficos; si las regiones sugeridas son inmunogénicas, podrían ser utilizadas para el desarrollo de un inmunógeno efectivo contra poblaciones de garrapatas R. microplus.   


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DOI: http://dx.doi.org/10.19136/era.a6n16.1832

ECOSISTEMAS Y RECURSOS AGROPECUARIOS(ECOSYSTEMS AND AGRICULTURAL RESOURCES), Year 6, Issue 16, January-April 2019, is a triannual journal edited, published and distributed by the Universidad Juárez Autónoma de Tabasco, Av. Universidad s/n, Zona de la Cultura, Col. Magisterial, Villahermosa, Centro, Tabasco, CP. 86040, Tel (993) 358 15 00, www.ujat.mx, era@ujat.mx., era@ujat.mx. Editor-in-chief: Efraín de la Cruz Lázaro. Copyright No. 04-2013-120514213600-203, ISSN: 2007-901X, both granted by the Instituto Nacional del Derecho de Autor (National Institute of Copyright), with certificate of title and content No. 16540 granted by the Secretaría de Gobernación(Ministry of the Interior). Individual responsible for the last update of this issue was journal Editorial Assistant Lic. Misael Hernández Martínez, Av. Universidad s/n, Zona de la Cultura, Col. Magisterial, Vhsa, Centro, Tabasco, Mex. C.P. 86040; date of last modification, December 31, 2018.

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