Detección de genes de resistencia antimicrobiana en aislados de Salmonella en la Región Laguna, México

Autores/as

  • Miguel Ángel Gallegos Robles
    • Formal Analysis
    • Writing – Original Draft Preparation
    • Conceptualization
    • Methodology
    • Writing – Review & Editing
  • Uriel González Salas UJED , Universidad Juárez del Estado de Durango image/svg+xml
    • Conceptualization
    • Formal Analysis
    • Methodology
    • Writing – Original Draft Preparation
    • Writing – Review & Editing
  • Eduardo Arón Flores Hernández
    • Methodology
    • Writing – Review & Editing
    • Conceptualization
    • Writing – Original Draft Preparation
  • Pablo Preciado Rangel
    • Writing – Review & Editing
    • Methodology
    • Formal Analysis
  • Nery Cecilia García de la Paz
    • Conceptualization
    • Writing – Review & Editing
    • Methodology
  • Roberto Sánchez Lucio
    • Writing – Review & Editing
    • Methodology
    • Conceptualization

DOI:

https://doi.org/10.19136/era.a13n1.4933

Palabras clave:

Pollo de engorda, patógenos trasmitidos por alimentos, métodos moleculares, PCR-RFLP, Salmonella

Resumen

La Región Laguna es conocida por su avicultura. Esta actividad conlleva riesgos zoonóticos. Uno de estos riesgos es la salmonela, responsable de una proporción significativa de muertes en todo el mundo. En este trabajo, se determinó la prevalencia específica de Salmonella en muestras de alimento para pollo, agua de bebederos y estiércol de pollo en granjas; así como la presencia de genes que confieren resistencia a antibióticos. Se analizaron un total de 240 muestras, de las cuales 80 resultaron positivas para Salmonella, siendo el único serotipo identificado S. Thyphimurium. El perfil genético de resistencia a antibióticos más frecuentemente observado, con un 26.25%, fue el tetraresistente ACSSu. Solo tres de los aislados eran portadores de los seis genes de resistencia a antibióticos, por lo que se pueden considerar multiresistentes. Los resultados indican que, en los entornos avícolas de la Región Laguna, hay cepas de Salmonella con patrones de multiresistencia a antibióticos.

Descargas

Los datos de descarga aún no están disponibles.

Referencias

Ali T, Mohammadali A, Katherine B, Janan SD, Shayan S (2025) Control of Salmonella in poultry: The role of host immunity and vaccines. Poultry Science 104(11): 105692 https://doi.org/10.1016/j.psj.2025.105692.

Balsalobre HB, Hernández-Godoy J (2004) Resistencias a antibióticos en Listeria monocytogenes y Salmonella enterica aislados de alimentos de origen animal. Revista de Salud Ambiental 4(1-2): 42-46.

Bolan NS, Szogi AA, Chuasavathi T, Seshadri B, Rothrock JRMJ, Panneerselvam P (2010) Uses and management of poultry litter. World´s Poultry Science Journal 66: 673-698.

Camacho EM, Serna A, Casadesus J (2005) Regulation of conjugal transfer by Lrp and dam methylation in plasmid R100. International Microbiology 8: 279-285.

Campos-Granados CM, Sierra Gómez PL del C, Hernández-Pérez CF, Ballesteros-Nova NE, Rubio-Lozano MS, Sánchez-Zamorano LM, Delgado-Suárez EJ. (2023) Fuertes perfiles de resistencia a antibióticos en Salmonella spp. aislada de carne de res molida en el centro de México. Veterinaria México OA. 10. http://dx.doi.org/10.22201/fmvz.24486760e.2023.1215

Collineau L, Phillips Ch, Chapman B, Agunos A, Fazil A, Reid-Smith RJ, Smith BA (2020) A within-flock model of Salmonella Heidelberg transmission in broiler chickens. Preventive Veterinary Medicine 174: 1-13. https://doi.org/10.1016/j.prevetmed.2019.104823

Contreras-Soto MB, Medrano-Félix JA, Ibarra-Rodríguez JR, Martínez-Urtaza J, Chaidez QC, Castro-del CampoN (2019) The last 50 years of Salmonella in Mexico: Sources of isolation and factors that influence its prevalence and diversity. Revista Bio Ciencias 6(nesp): e540. https://doi.org/10.15741/revbio.06.nesp.e540

Dauga C, Zabrovskaia A, Grimont PAD (1998) Restriction fragment length polymorphism analysis of some flagellin genes of Salmonella enterica. Journal of Clinical Microbiology 36(10): 2835–2843.

Davila MRC, Ortiz CVA (2022) Influencia de Salmonella Pullorum y S. Gallinarum en la producción avícola y la salud pública. Boletín de Malariología y Salud Ambiental 62(4): 623-630.

Denagamage T, Jayarao B, Patterson P, Wallner-Pendleton E, Kariyawasam S (2015) Risk factors associated with Salmonella in laying hen farms: Systematic review of observational studies. Avian Diseases 59(2): 291-302. https://doi.org/10.1637/10997-120214-Reg

Deng X, Li S, Xu T, Zhou Z, Moore MM, Timme R, Zhao S, Lane Ch, Dinsmore BA, Weill FX, Fields PI (2025) Salmonella serotypes in the genomic era: simplified Salmonella serotype interpretation from DNA sequence data. Applied and Environmental Microbiology 91(3): e02600-24. https://doi.org/10.1128/aem.02600-24

Di Conza J, Power P, Gutkind G (2013) Intercambio de mecanismos de resistencia entre bacterias gram negativas. Revista Farmacéutica 155 (1-2): 57-69.

Frederick A, Huda N (2011) Salmonellas, Poultry House Environments and Feeds: A Review. Journal of Animal and Veterinary Advances 10 (5): 679-685.

Gallegos-Robles MA, Morales-Loredo A, Alvarez-Ojeda G, Vega-P A, Chew-M Y, Velarde S, Fratamico P (2008) Identification of Salmonella Serotypes Isolated from Cantaloupe and Chile Pepper Production Systems in Mexico by PCR–Restriction Fragment Length Polymorphism. Journal of Food Protection 71 (11): 2217–2222.

Golden CE, Rothrock MJ, Mishra A (2021) Mapping foodborne pathogen contamination throughout the conventional and alternative poultry supply chains. Poultry Science 100 (7): 101-157. https://doi.org/10.1016/j.psj.2021.101157.

Griffiths N (2011) Best practice guidelines for using poultry litter on pastures. State of New South Wales through NSW Department of Primary Industries. https://www.dpi.nsw.gov.au/__data/assets/pdf_file/0004/140359/Best-practice-guidelines-for-using-poultry-litter-on-pastures.pdf. Data consulted: September 2025.

Heyndrickx M, Vandekerchove D, Herman L, Rollier I, Grijspeerdt K, De Zutter L (2002) Routes for Salmonella contamination of poultry meat: epidemiological study from hatchery to slaughterhouse. Epidemiology and Infection 129: 253-265. https://doi.org/10.1017/s0950268802007380

Kyakuwaire M, Olupot G, Amoding A, Nkedi-Kizza P, Basamba TA (2019) How safe is chicken litter for land Application as an organic fertilizer?: A review. International Journal of Environmental Research and Public Health 16: 3521. https://doi.org/10.3390/ijerph16193521

Lanyasunya TP, Rong WH, Abdulrazak SA, Kaburu PK, Makori JO, Onyango TA, Mwangi DM (2006) Factors limiting the use of poultry manure as protein supplement for dairy cattle on smallholder farms in Kenya. International Journal of Poultry Science 5(1): 75-80.

Li Q, Cheng W, Zhang D, Yu T, Yin Y, Ju H, Ding S (2012) Rapid and sensitive strategy for Salmonella detection using an InvA gene-based electrochemical DNA sensor. International Journal of Electrochemical Science 7(1): 844-856. https://doi.org/10.1016/S1452-3981(23)13380-3

Liebert CA, Hall RM, Summers AO (1999) Transposon Tn21, flagship of the floating genome. Microbiology and Molecular Biology Reviews 63(3): 507-522. https://doi.org/10.1128/mmbr.63.3.507-522.1999

Little CL, Richardson JF, Owen RJ, De Pinna E, Threlfall EJ (2008) Campylobacter and Salmonella in raw red meats in the United Kingdom: Prevalence, characterization and antimicrobial resistance pattern, 2003–2005. Food Microbiology 25: 538–543. https://doi.org/10.1016/j.fm.2008.01.001

Liu B, Zhang X, Ding X, Bin P, Zhu G (2023) The vertical transmission of Salmonella Enteritidis in a One-Health context. One Health 16: 100469. http://doi.org/10.1016/j.onehlt.2022.100469

Menconi A, Pumford NR, Morgan MJ, Bielke LR, Kallapura G, Latorre JD, Wolfenden AD, Hernandez-Velasco X, Hargis BM, Tellez G (2014) Effect of chitosan on Salmonella Typhimurium in broiler chickens. Foodborne Pathogens and Disease 11(2): 165-169. https://doi.org/10.1089/fpd.2013.1628

Mezali L, Hamdi TM (2012) Prevalence and antimicrobial resistance of Salmonella Isolated from meat and meat products in algiers (Algeria). Foodborne Pathogens and Disease 9(6): 522-529. https://doi.org/10.1089/fpd.2011.1032

Mohammad-Maruf B, Md-Saydur R (2024) Salmonella in the environment: A review on ecology, antimicrobial resistance, seafood contaminations, and human health implications. Journal of Hazardous Materials Advances 13 (2024) 100407. https://doi.org/10.1016/j.hazadv.2024.100407

Muniz E, Mesa D, Cuaspa R, Souza AM, Santin E (2014) Presence of Salmonella spp. in reused broiler litter. Revista Colombiana de Ciencias Pecuarias 27: 12-17.

NLM. National Library of Medicine (2026) Salmonella enterica subsp. enterica serovar Typhimurium strain SGSC3029 phase 1 flagellin (fliC) gene, complete cds. https://www.ncbi.nlm.nih.gov/nuccore/AY649721.1. Data Consulted: February 2026.

Pandey S, Doo H, Keum GB, Kim ES, Kwak J, Ryu S, Choi Y, Kang J, Kim S, Lee NR, Oh KK, Lee JH, Kim HB (2024) Antibiotic resistance in livestock, environment and humans: One health perspective. Journal of Animal Science and Technology 66(2): 266-278. https://doi.org/10.5187/jast.2023.e129

Park SH, Jarquin R, Hanning I, Almeida G, Ricke SC (2011) Detection of Salmonella spp. survival and virulence in poultry feed by targeting the hilA gene. Journal of Applied Microbiology 111(2): 426-432. https://doi.org/10.1111/j.1365-2672.2011.05054.x

Peterson E, Kaur P (2018) Antibiotic resistance mechanisms in bacteria: Relationships between resistance determinants of antibiotic producers, environmental bacteria, and clinical pathogens. Frontiers in Microbiology 9: 1-21. https://doi.org/10.3389/fmicb.2018.02928

Randall LP, Cooles SW, Osborn MK, Piddock LJV, Woodward MJ (2004) Antibiotic resistance genes, integrons and multiple antibiotic resistances in thirty-five serotypes of Salmonella enterica isolated from humans and animals in the UK. Journal of Antimicrobial Chemotherapy 53(2): 208-216. https://doi.org/10.1093/jac/dkh070

Rhan K, De Grandis SA, Clarke RC, McEwen SA, Galan JE, Ginochio C, Curtiss III R, Gyles CL (1992) Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella. Molecular and Cellular Probes 6: 271-279. https://doi.org/10.1016/0890-8508(92)90002-F

Ribera A, Roca I, Ruiz J, Gilbert I, Villa J (2003) Partial characterization of a transposon containing the tet(A) determinant in a clinical isolate of Acinetobacter baumannii. Journal of Antimicrobial Chemotherapy 52(3): 477-480. https://doi.org/10.1093/jac/dkg344

Robinson EJ, Gregory J, Mulvenna V, Segal Y, Sullivan SG (2022) Effect of temperature and rainfall on sporadic salmonellosis notifications in Melbourne, Australia 2000-2019: A time-series analysis. Foodborne Pathogens and Disease 19(5): 341-348. https://doi.org/10.1089/fpd.2021.0093

Sakeena MHF, Bennett AA, McLachlan AJ (2018) Non-prescription sales of antimicrobial agents at community pharmacies in developing countries: a systematic review. International Journal of Antimicrobial Agents 52(6): 771-782. https://doi.org/10.1016/j.ijantimicag.2018.09.022

Shaji S, Selvaraj RK, Shanmugasundaram R (2023) Salmonella infection in poultry: a review on the pathogen and control strategies. Microorganisms 11(11): 2814. https://doi.org/10.3390/microorganisms11112814

Soria MC, Soria MA, Bueno DJ (2013) A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking water. Poultry Science 92(1): 225-232. https://doi.org/10.3382/ps.2012-02254

Soria MC, Soria MA, Bueno DJ, Colazo JL (2011) A comparative study of culture methods and polymerase chain reaction assay for Salmonella detection in poultry feed. Poultry Science 90(11): 2606–2618. https://doi.org/10.3382/ps.2011-01548

Talavera RM, Varela GJA, Reyes RNE, Lagunas BS, Valladares CB, Alonso FMU, Velázquez OV (2011) Antibiotic resistance of Salmonella spp strain genotypes isolated from pigs slaughtered at abattoirs in the Estado de México. Veterinaria Mexico 42(4): 269-276.

USDA-FSIS MLG 4.15 (2024) Isolation and Identification of Salmonella from Meat, Poultry, Pasteurized Egg, Siluriformes (Fish) Products and Carcass and Environmental Sponges. United States Department of Agriculture Food Safety and Inspection Service. https://www.fsis.usda.gov/sites/default/files/media_file/documents/MLG_4.15.pdf. Data consulted: August 2025.

Uzzau S, Brown DJ, Wallis T, Rubino S, Leori G, Bernard S, Casadesús J, Platt DJ, Olsen JE (2000) Host adapted serotypes of Salmonella enterica. Epidemiology & Infection 125(2): 229-255. https://doi.org/10.1017/S0950268899004379

Velázquez AMC, Irigoyen CME, Delgadillo VJ (2012) Salud muscular y prevención de sarcopenia: el efecto de la proteína, leucina y β-hidroxi-β-metilbutirato. Revista Metabolismo Óseo y Mineral 10(2): 98-102.

Wang X, Biswas S, Paudyal N, Pan H, Li X, Fang W, Yue M (2019) Antibiotic resistance in Salmonella typhimurium isolates recovered from the food chain through national antimicrobial resistance monitoring system between 1996 and 2016. Frontiers in Microbiology 10: 985. https://doi.org/10.3389/fmicb.2019.00985

White A, Hughes JM (2019) Critical importance of a one health approach to antimicrobial resistance. EcoHealth 16: 404-409. https://doi.org/10.1007/s10393-019-01415-5

Winfield MD, Groisman EA (2003) Minireview; role of non-host environments in the lifestyles of Salmonella and Escherichia coli. Applied and Environmental Microbiology 69(7): 3687-3694. https://doi.org/10.1128/AEM.69.7.3687-3694.2003

Zhao S, Datta AR, Ayers S, Friedman S, Walker RD, White DG (2003) Antimicrobial-resistant Salmonella serovars isolated from imported foods. International Journal of Food Microbiology 84(1): 87-92. https://doi.org/10.1016/S0168-1605(02)00402-6

Zizza A, Fallucca A, Guido M, Restivo V, Roveta M and Trucchi C (2024) Foodborne Infections and Salmonella: Current primary prevention tools and future perspectives. Vaccines 13: 29. https://doi.org/10.3390/vaccines13010029

Descargas

Publicado

2026-03-25

Número

Sección

NOTAS CIENTÍFICAS

Cómo citar

Gallegos Robles, M. Ángel, González Salas, U., Flores Hernández, E. A., Preciado Rangel, P., García de la Paz, N. C., & Sánchez Lucio, R. (2026). Detección de genes de resistencia antimicrobiana en aislados de Salmonella en la Región Laguna, México. Ecosistemas Y Recursos Agropecuarios, 13(1). https://doi.org/10.19136/era.a13n1.4933

Artículos más leídos del mismo autor/a