Differentiation of apomictic and sexual genotypes of Brachiaria spp., using molecular markers

Autores/as

  • Jaime Pblete-Vargas Programa de Semillas, Colegio de Postgraduados Campus Montecillo. Km 36.5 Carretera México- Texcoco, CP. 56230, Montecillo, Texcoco, Estado de México, México.
  • Ernestina Valadez-Moctezuma Universidad Autónoma Chapingo
  • Gabino García-de los Santos Programa de Semillas, Colegio de Postgraduados Campus Montecillo. Km 36.5 Carretera México- Texcoco, CP. 56230, Montecillo, Texcoco, Estado de México, México.
  • Carlos Martínez-Flores Programa Global de Maíz. Centro Internacional de Mejoramiento de Maíz y Trigo. Carretera México-Veracruz. Km. 45, Colonia El Batán, CP. 56237, Texcoco, Estado de México, México.
  • Armando Peralta-Martínez Agroproductos de Iguala, S.A. de C.V. Hidalgo No. 23, Col. Centro, CP. 40400, Iguala, Guerrero, México.

DOI:

https://doi.org/10.19136/era.a5n13.1180

Palabras clave:

AFLPs, CAPS, DNA, forraje, polimorfismo.

Resumen

Some species of Brachiaria are cultivated in the tropics because of their high productivity and drought resistance; their apomictic hybrids are of interest because of the almost null segregation and xation of the hybrid vigor in seeds. In this research, Amplied Fragment Length Polymorphism (AFLP) and Cleaved Amplied Polymorphic Sequence (CAPS) markers were used to dierentiate apomictic tetraploid and sexual diploid Brachiaria parents and their progeny. Polymorphism detected with AFLP was 91.3% and with CAPS 79.7%. Phenograms dierentiated each genotype and the clustering of parents and their progeny was by their degree of genetic relatedness. AFLP did not separate the apomictic genotypes from the sexual ones, but CAPS markers did so through the C15-8 marker. Some apomictic genotypes of the progeny inherited it from their B. ruziziensis sexual mother, which may be related to some segregating character of the parental line.

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Biografía del autor/a

Ernestina Valadez-Moctezuma, Universidad Autónoma Chapingo

Responsable del Laboratorio de Biología Molecular del Departamento de Fitotecnia de la Universidad Autonóma Chapingo.

Citas

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Publicado

2018-01-01

Cómo citar

Pblete-Vargas, J., Valadez-Moctezuma, E., García-de los Santos, G., Martínez-Flores, C., & Peralta-Martínez, A. (2018). Differentiation of apomictic and sexual genotypes of Brachiaria spp., using molecular markers. Ecosistemas Y Recursos Agropecuarios, 5(13), 71–80. https://doi.org/10.19136/era.a5n13.1180

Número

Sección

NOTAS CIENTÍFICAS