OPTIMAL CONCENTRATION OF KANAMYCIN AS A SELECTIVE AGENT FOR THE TRANSFORMATION OF Musa cv. “GRAND NAIN”

BH Chi-Manzanero, POM Acereto-Escoffié, E Castaño, LC Rodríguez-Zapata

Abstract


The effect of different kanamycin concentrations on the growth and morphogenesis of meristematic tissue in the banana “Grand Nain” is presented in this study. The results obtained allowed us to establish the kanamycin concentration to be used for the selection of explants transformed by the nptII gene. This result will allow us to select the banana explants transformed by the integration of the nptII gene.

Keywords


Kanamycin; Musa; meristematic tissue



DOI: http://dx.doi.org/10.19136/era.a26n1.187

ECOSISTEMAS Y RECURSOS AGROPECUARIOS(ECOSYSTEMS AND AGRICULTURAL RESOURCES), Year 6, Issue 16, January-April 2019, is a triannual journal edited, published and distributed by the Universidad Juárez Autónoma de Tabasco, Av. Universidad s/n, Zona de la Cultura, Col. Magisterial, Villahermosa, Centro, Tabasco, CP. 86040, Tel (993) 358 15 00, www.ujat.mx, era@ujat.mx., era@ujat.mx. Editor-in-chief: Efraín de la Cruz Lázaro. Copyright No. 04-2013-120514213600-203, ISSN: 2007-901X, both granted by the Instituto Nacional del Derecho de Autor (National Institute of Copyright), with certificate of title and content No. 16540 granted by the Secretaría de Gobernación(Ministry of the Interior). Individual responsible for the last update of this issue was journal Editorial Assistant Lic. Misael Hernández Martínez, Av. Universidad s/n, Zona de la Cultura, Col. Magisterial, Vhsa, Centro, Tabasco, Mex. C.P. 86040; date of last modification, December 31, 2018.

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