Absence of Concordance between Polyembryony and Apomixis in Maize Confirmed through DNA Sequencing

Alondra Jacqueline Gutiérrez-López, José Espioza-Velázquez, Adriana Carolina Flores-Gallegos, Alfonso López-Benitez, Norma Angélica Ruiz-Torres, Raúl Rodríguez-Herrera

Abstract


Maize (Zea mays L.) polyembryony is a useful feature for genetic improvement of this specie, not only by its potential to generate multiple plants per seed, but also by its influence on increasing of fatty acids and amino acids content in the grain. It has been considered a possible association between apomixis and polyembryony in maize. With the objective to evidence the relation between apomixes and polyembryony, were used sequences of internal transcribed spacers (ITS), and intergenic spacers (IGS) and amplification of simple repeated sequences (SSR). The analyses were performed in 5 families derived from the IMM-UAAAN-BAP (“D”) maize population. Within each of the families were analysed the female parent plant, and two types of progenies (individual and polyembryonic). Nucleotide sequences and genotypic class were compared and also a molecular variation analysis was performed. In these analyses only a close but not identical relationship between polyembryonic plants was found. With the use of these techniques, it was demonstrated that reproduction of the maize plants is of a sexual type, and that based on the molecular markers used, no evidence was obtained about the probable relation- ship of a common genetic basis between polyembryony and apomixis. Sequencing of the ITS and IGS regions, and use of SSR microsatellites of different chromosomes, was a practical and economical tool for the assessment of similarity between genotypes.


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DOI: http://dx.doi.org/10.19136/era.a6n18.2111

ECOSISTEMAS Y RECURSOS AGROPECUARIOS(ECOSYSTEMS AND AGRICULTURAL RESOURCES), Year 6, Issue 16, January-April 2019, is a triannual journal edited, published and distributed by the Universidad Juárez Autónoma de Tabasco, Av. Universidad s/n, Zona de la Cultura, Col. Magisterial, Villahermosa, Centro, Tabasco, CP. 86040, Tel (993) 358 15 00, www.ujat.mx, era@ujat.mx., era@ujat.mx. Editor-in-chief: Efraín de la Cruz Lázaro. Copyright No. 04-2013-120514213600-203, ISSN: 2007-901X, both granted by the Instituto Nacional del Derecho de Autor (National Institute of Copyright), with certificate of title and content No. 16540 granted by the Secretaría de Gobernación(Ministry of the Interior). Individual responsible for the last update of this issue was journal Editorial Assistant Lic. Misael Hernández Martínez, Av. Universidad s/n, Zona de la Cultura, Col. Magisterial, Vhsa, Centro, Tabasco, Mex. C.P. 86040; date of last modification, December 31, 2018.

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