DEVELOPMENT OF A MODEL FOR IN VITRO CULTURE OF Vallisneria americana Michx.

V Ruiz-Carrera, AJ Sánchez

Abstract


An exploratory experimental design was developed to establish a model for Vallisneria americana Michx. following conventional in vitro culture procedures for aquatic vegetation. The design included five experiments with stages of presterilization or washing with 0.1% Tween 20R, sterilization with NaClO and poststerilization of explants (leaf base, rhizome, caulinar apex and seeds), and culture in monophasic or biphasic nutrient media. Leaves and rhizo-mes were sterilized with 0.3 and 0.6% NaClO, and contact times of 2.5 and 5 min. Both explants cultured in gellified monophasic White medium with 58.4 mM sacarose and a pH of 7.5 presented on average a 60% contamination by translucid lipidic exudates, followed by darkening. The presterilization of leaves and rhizomes caused the production of exudates, and the addition of 0.01% ascorbic acid to the nutrient medium caused its inhibition. Comparing the White medium with the 0.5 MS medium, both with 0.88 x 10-3mM of bencyladenine, asepsia of explants was impro-ved in White and leaf pigmentation was significantly favored in 0.5 MS, although no explant presented regeneration. Presterilization was omitted in the experiments with caulinar apices and seeds, and sterilization was carried out with 0.6% NaClO for 15 min. The monophasic culture of caulinar apices in liquid 0.5 MS with doses from 0.0 to 8.88 x 10-33 mM of bencyladenine regenerated leaves but resulted in excessive contamination and death.

Keywords


Aseptic culture, viable explant, biphasic germination, Vallisneria americana, wild celery



DOI: http://dx.doi.org/10.19136/era.a24n3.256

ECOSISTEMAS Y RECURSOS AGROPECUARIOS(ECOSYSTEMS AND AGRICULTURAL RESOURCES), Year 6, Issue 16, January-April 2019, is a triannual journal edited, published and distributed by the Universidad Juárez Autónoma de Tabasco, Av. Universidad s/n, Zona de la Cultura, Col. Magisterial, Villahermosa, Centro, Tabasco, CP. 86040, Tel (993) 358 15 00, www.ujat.mx, era@ujat.mx., era@ujat.mx. Editor-in-chief: Efraín de la Cruz Lázaro. Copyright No. 04-2013-120514213600-203, ISSN: 2007-901X, both granted by the Instituto Nacional del Derecho de Autor (National Institute of Copyright), with certificate of title and content No. 16540 granted by the Secretaría de Gobernación(Ministry of the Interior). Individual responsible for the last update of this issue was journal Editorial Assistant Lic. Misael Hernández Martínez, Av. Universidad s/n, Zona de la Cultura, Col. Magisterial, Vhsa, Centro, Tabasco, Mex. C.P. 86040; date of last modification, December 31, 2018.

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