Phylogenetic analysis of Quercus L. species with three DNA barcode genetic markers

The phylogeny of Quercus L. species

Authors

  • Flor Cristina Pacheco-Reyes Estudiante de Doctorado en Recursos Fitogenéticos para Zonas Áridas, Universidad Autónoma Agraria Antonio Narro
  • Lihua Wei Laboratorio de Biomedicina Molecular, Centro de Biotecnología Genómica, Instituto Politécnico Nacional
  • Miguel Angel Perez-Rodriguez Departamento de Botánica, Universidad Autónoma Agraria Antonio Narro

DOI:

https://doi.org/10.19136/era.a8n2.2831

Abstract

The genus, Quercus L, Fagaceae has great relevance in terms of diversity, ecological and economic importance. However, the recorded taxonomic
information does not always distinguish the classification. In this study, three DNA regions (rbcL, matK and ITS2) were used as barcodes to identify species of the genus Quercus. The analyzed data (56 rbcl, 51 ITS and 61 matK sequences) corresponded to 92 species (11 are from Mexican, and 81 are from Eurasia and North America). The universal primers (rbcL and ITS2) amplified 100% of Mexican oak samples. The BLAST analysis of the rbcL and ITS2 fragments of Mexican oaks, showed a high identification ability with 98.1 and 98.3% respectively. Genetic distance-based method resulted in extremely low discrimination rates, possibly due to overlapping genetic distances, as well as frequent inter-species hybridization and
a low rate of variation. The neighbor-joining method provided a high discriminatory ability with matK (81.96%) and ITS2 (80.39%) markers, generating groupings based on geographic regions and sections of the Quercus subgenus in phylogenetic trees. According to the overall performance, ITS2 and matK are recommended to use as the most suitable DNA barcodes to identify Quercus species.

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Published

2021-06-25

Issue

Section

SCIENTIFIC ARTICLE

How to Cite

Pacheco-Reyes, F. C. ., Wei, L. ., & Perez-Rodriguez, M. A. (2021). Phylogenetic analysis of Quercus L. species with three DNA barcode genetic markers: The phylogeny of Quercus L. species. Ecosistemas Y Recursos Agropecuarios, 8(2). https://doi.org/10.19136/era.a8n2.2831

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