Hidroxiapatita de calcio mejora la calidad forrajera y compuestos bioactivos de alfalfa
DOI:
https://doi.org/10.19136/era.a13n2.5073Palabras clave:
Medicago sativa L., nanofertilizante, dosis-respuesta, calidad de forraje, fibra detergenteResumen
La producción sostenible de forrajes exige estrategias que mejoren la calidad nutricional y la eficiencia en el uso de recursos. En alfalfa (Medicago sativa L.), el fósforo (P) es un nutriente esencial involucrado en la transferencia de energía, la fotosíntesis, el crecimiento radicular y la síntesis de compuestos estructurales y nitrogenados, procesos que influyen directamente en el rendimiento y valor nutritivo del forraje. Sin embargo, los fertilizantes fosfatados convencionales suelen presentar baja eficiencia, especialmente en suelos calcáreos y de pH alto, donde gran parte del P se inmoviliza o pierde disponibilidad, limitando su absorción y reduciendo la sostenibilidad productiva.La hidroxiapatita (Ca₁₀(PO₄)₆(OH)₂) surge como alternativa por ser un fosfato cálcico de liberación gradual capaz de aportar simultáneamente fósforo y calcio. Este estudio evaluó el efecto de nanopartículas de hidroxiapatita de calcio (nHAp) aplicadas vía foliar en alfalfa sobre variables fisiológicas, nutricionales y bioactivas. Se utilizó un diseño completamente al azar con seis concentraciones (0, 100, 200, 300, 400 y 500 mg L⁻¹) y cinco repeticiones. La dosis de 100 mg L⁻¹ incrementó la materia seca y la proteína cruda, redujo las fracciones fibrosas, lo que indica mejora en calidad nutricional. Por su parte, 300 mg L⁻¹ elevó el contenido de clorofilas, sugiriendo mayor eficiencia fotosintética. Las dosis altas (400–500 mg L⁻¹) aumentaron sólidos solubles, flavonoides y la capacidad antioxidante. En conjunto, los resultados indican que las nHAp son una herramienta prometedora para mejorar la calidad nutricional de la alfalfa y favorecer sistemas forrajeros sostenibles.
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Abdelmigid HM, Morsi MM, Hussien NA, Alyamani AA, Alhuthal NA, Albukhaty S (2022) Green synthesis of phosphorous-containing hydroxyapatite nanoparticles (nHAP) as a novel nano-fertilizer: preliminary assessment on pomegranate (Punica granatum L.). Nanomaterials 12: 1527. https://doi.org/10.3390/nano12091527
Ahmad-Qasem MH, Barrajón-Catalán E, Micol V, Mulet A, García-Pérez JV (2013) Influence of freezing and dehydration of olive leaves (var. Serrana) on extract composition and antioxidant potential. Food Research International 50: 189-196. https://doi.org/10.1016/j.foodres.2012.10.028
Ahmed M, Ahmad S, Abbas G, Hussain S, Hoogenboom G (2024) Alfalfa system. In: Cropping Systems Modeling Under Changing Climate. Springer, Singapore. https://doi.org/10.1007/978-981-97-0331-9_13
Ammar M, Ashraf S, Baltrusaitis J (2025) Hydroxyapatite/urea hybrid materials: what is the basis for the enhanced nutrient efficiency? Environmental Science: Advances 4: 77-89. https://doi.org/10.1039/D4VA00197D
Anjum NA, Sharma P, Gill SS, Hasanuzzaman M, Khan EA, Kachhap K (2016) Catalase and ascorbate peroxidase-representative H2O2-detoxifying heme enzymes in plants. Environmental Science and Pollution Research 23: 19002-19029. https://doi.org/10.1007/s11356-016-7309-6
Atumo TT, Kauffman R, Gemiyo Talore D, Abera M, Tesfaye T, Tunkala BZ (2021) Adaptability, forage yield and nutritional quality of alfalfa (Medicago sativa) genotypes. Sustainable Environment 7: 1895475. https://doi.org/10.1080/27658511.2021.1895475
Bera I, O’Sullivan M, Flynn D, Shields DC (2023) Relationship between protein digestibility and the proteolysis of legume proteins during seed germination. Molecules 28: 3204. https://doi.org/10.3390/molecules28073204
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology 28: 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
Droushiotis D (2023) Forage legumes: Quality, utilization and prospects. Agronomy 13(2): 315. https://doi.org/10.3390/agronomy13020315
Elsayed AA, Ahmed EG, Taha ZK, Farag HM, Hussein MS, AbouAitah K (2022) Hydroxyapatite nanoparticles as novel nano-fertilizer for production of rosemary plants. Scientia Horticulturae 295: 110851. https://doi.org/10.1016/j.scienta.2021.110851
Eshdat Y, Holland D, Faltin Z, Ben-Hayyim G (1997) Plant glutathione peroxidases. Physiologia Plantarum 100: 234-240. https://doi.org/10.1111/j.1399-3054.1997.tb04779.x
Feng Y, Shi Y, Zhao M, Shen H, Xu L, Luo Y (2022) Yield and quality properties of alfalfa (Medicago sativa L.) and their influencing factors in China. European Journal of Agronomy 141: 126637. https://doi.org/10.1016/j.eja.2022.126637
Flores-Hernández EA, Lira-Saldívar RH, Ruiz-Torres NA, García-López JI, Moreno-Reséndez A, Rodríguez-Dimas N (2021) Síntesis de nanopartículas de hidroxiapatita y su efecto en plántulas de Raphanus sativus. Ecosistemas y Recursos Agropecuarios 8: e2747. https://doi.org/10.19136/era.a8n1.2747
Gaucin-Delgado JM, Preciado-Rangel P, González-Salas U, Sifuentes-Ibarra E, Núñez-Ramírez F, Vidal JAO (2021) La biofortificación con selenio mejora los compuestos bioactivos y la actividad antioxidante en chile jalapeño. Revista Mexicana de Ciencias Agrícolas 12: 1339-1349. https://doi.org/10.29312/remexca.v12i8.3066
Golkar P, Vázquez-Núñez E, Peralta-Videa JR (2026) Nano-elicitation approaches to enhance secondary metabolites in medicinal plant cell cultures. Plants 15: 46. https://doi.org/10.3390/plants15010046
Hasan M, Mehmood K, Mustafa G, Zafar A, Tariq T, Hassan SG (2021) Phytotoxic evaluation of phytosynthesized silver nanoparticles on lettuce. Coatings 11: 225. https://doi.org/10.3390/coatings11020225
Hasanuzzaman M, Bhuyan MB, Anee TI, Parvin K, Nahar K, Mahmud JA, Fujita M (2019b) Regulation of ascorbate-glutathione pathway in mitigating oxidative damage in plants under abiotic stress. Antioxidants 8: 384. https://doi.org/10.3390/antiox8090384
Hasanuzzaman M, Nahar K, Anee TI, Fujita M (2017a) Glutathione in plants: biosynthesis and physiological role in environmental stress tolerance. Physiology and Molecular Biology of Plants 23: 249-268. https://doi.org/10.1007/s12298-017-0422-2
Hashem AH, El-Sayyad GS, Al-Askar AA, Marey SA, AbdElgawad H, Abd-Elsalam KA, Saied E (2023) Watermelon rind mediated biosynthesis of bimetallic selenium-silver nanoparticles: characterization, antimicrobial and anticancer activities. Plants 12: 3288. https://doi.org/10.3390/plants12183288
Islam S (2025) Toxicity and transport of nanoparticles in agriculture: effects of size, coating, and aging. Frontiers in Nanotechnology 7: 1622228. https://doi.org/10.3389/fnano.2025.1622228
Izuafa A, Chimbekujwo KI, Raji RO, Oyewole OA, Oyewale RO, Abioye OP (2025) Application of nanoparticles for targeted management of pests, pathogens and disease of plants. Plant Nano Biology 100177. https://doi.org/10.1016/j.plana.2025.100177
Jia C, Zhao F, Wang X, Han J, Zhao H, Liu G, Wang Z (2018) Genomic prediction for 25 agronomic and quality traits in alfalfa (Medicago sativa). Frontiers in Plant Science 9: 1220. https://doi.org/10.3389/fpls.2018.01220
Kah M, Kookana RS, Gogos A, Bucheli TD (2019) A critical evaluation of nanopesticides and nanofertilizers against their conventional analogues. Nature Nanotechnology 14(6): 517-522. https://doi.org/10.1038/s41565-019-0431-1
Kaya C, Akram NA, Sürücü A, Ashraf M (2019) Alleviating effect of nitric oxide on oxidative stress and antioxidant defence system in pepper (Capsicum annuum L.) plants exposed to cadmium and lead toxicity applied separately or in combination. Scientia Horticulturae 255: 52-60. https://doi.org/10.1016/j.scienta.2019.05.029
Koç ÜG, İlhan D (2025) CaO nanoparticles improve salt stress tolerance in diploid and tetraploid alfalfa (Medicago sativa L.) by modulating miRNA expression and physiological responses. Plant Cell, Tissue and Organ Culture 160: 75. https://doi.org/10.1007/s11240-025-02969-9
Kopittke PM, Menzies NW, Wang P, McKenna BA, Lombi E (2019) Soil and the intensification of agriculture for global food security. Environment International 132: 105078. https://doi.org/10.1016/j.envint.2019.105078
Kumar CV, Karthick V, Kumar VG, Inbakandan D, Rene ER, Suganya KU (2022) The impact of engineered nanomaterials on the environment: release mechanism, toxicity, transformation, and remediation. Environmental Research 212: 113202. https://doi.org/10.1016/j.envres.2022.113202
Landa P, Müller K, Přerostová S, Petrová Š, Moťková K, Vaněk T, Soudek P (2024) Effect of nano-hydroxyapatite and phosphate on thorium toxicity–Arabidopsis transcriptomic study. Environmental and Experimental Botany 217: 105573. https://doi.org/10.1016/j.envexpbot.2023.105573
Lezoche M, Hernandez JE, Díaz MDM, Panetto H, Kacprzyk J (2020) Agri-food 4.0: A survey of the supply chains and technologies for the future agriculture. Computers in Industry 117: 103187. https://doi.org/10.1016/j.compind.2020.103187
Liu R, Lal R (2014) Synthetic apatite nanoparticles as a phosphorus fertilizer for soybean (Glycine max). Scientific Reports 4: 5686. https://doi.org/10.1038/srep05686
Lynch JM, Barbano DM (1999) Kjeldahl nitrogen analysis as a reference method for protein determination in dairy products. Journal of AOAC International 82: 1389-1398. https://doi.org/10.1093/jaoac/82.6.1389
Lynch JP (2019) Root phenotypes for improved nutrient capture: An underexploited opportunity for global agriculture. New Phytologist 223(2): 548-564. https://doi.org/10.1111/nph.15738
Madanayake NH, Adassooriya NM, Salim N (2021) The effect of hydroxyapatite nanoparticles on Raphanus sativus with respect to seedling growth and two plant metabolites. Environmental Nanotechnology, Monitoring & Management 15: 100404. https://doi.org/10.1016/j.enmm.2020.100404.
Malea P, Emmanouilidis A, Kevrekidis DP, Moustakas M (2022) Copper uptake kinetics and toxicological effects of ionic Cu and CuO nanoparticles on the seaweed Ulva rigida. Environmental Science and Pollution Research 29: 57523-57542. https://doi.org/10.1007/s11356-022-19571-7
Marchiol L, Filippi A, Adamiano A, Degli Esposti L, Iafisco M, Mattiello A, Petrussa E, Braidot E (2019) Influence of hydroxyapatite nanoparticles on germination and plant metabolism of tomato (Solanum lycopersicum L.): Preliminary evidence. Agronomy 9: 161. https://doi.org/10.3390/agronomy9040161
Martín-Cardoso H, San Segundo B (2025) Impact of nutrient stress on plant disease resistance. International Journal of Molecular Sciences 26: 1780. https://doi.org/10.3390/ijms26041780
Mendoza-Hernández JM, Zermeño-González A, Covarrubias-Ramírez JM, Cortés-Bracho JJ (2013) Proyecciones climáticas para el estado de Coahuila usando el modelo PRECIS bajo dos escenarios de emisiones. Agrociencia 47: 523-537.
Mondéjar-López M, García-Simarro MP, Navarro-Simarro P, Gómez-Gómez L, Ahrazem O, Niza E (2024) Una revisión sobre la encapsulación de compuestos ecológicos en nanopartículas poliméricas naturales como nanoagroquímicos de nueva generación para la agricultura y la gestión sostenible de cultivos. International Journal of Biological Macromolecules 180: 136030. https://doi.org/10.1016/j.ijbiomac.2024.136030
Montalvo D, Degryse F, McLaughlin MJ (2020) Natural and novel nano-fertilizers for sustainable agriculture. In: Fernández MG, Sánchez LR (eds) Nano-enabled sustainable and precision agriculture. Academic Press. pp. 179-200. https://doi.org/10.1016/B978-0-12-817995-5.00008-5
Moorby JM, Fraser MD (2021) New feeds and new feeding systems in intensive and semi-intensive forage-fed ruminant livestock systems. Animal 15: 100297. https://doi.org/10.1016/j.animal.2021.100297
Mustapha T, Misni N, Ithnin NR, Daskum AM, Unyah NZ (2022) A review on plants and microorganisms mediated synthesis of silver nanoparticles, role of plants metabolites and applications. International Journal of Environmental Research and Public Health 19: 674. https://doi.org/10.3390/ijerph19020674
Nagata M, Yamashita I (1992) Simple method for simultaneous determination of chlorophyll and carotenoids in tomato fruit. Nippon Shokuhin Kogyo Gakkaishi 39: 925-928. https://doi.org/10.3136/nskkk1962.39.925
Peyraud JL, Astigarraga L (2023) Review of the effect of dairy farming practices on milk and beef quality. Animal 17: 100781. https://doi.org/10.1016/j.animal.2022.100781
Quiñones-Muñoz TA, Villanueva-Rodríguez SJ, Torruco-Uco JG (2022) Nutraceutical properties of Medicago sativa L., Agave spp., Zea mays L. and Avena sativa L.: A review of metabolites and mechanisms. Metabolites 12: 806. https://doi.org/10.3390/metabo12090806
Riaz M, Ali Q, Yan L (2025) Calcium–L-aspartate nanoparticles mitigate Boron toxicity in rice seedlings by modulating physiological, antioxidant, and cell wall mechanisms. Scientific Reports 15: 42439. https://doi.org/10.1038/s41598-025-26516-w.
Rivero-Montejo SDJ, Vargas-Hernandez M, Torres-Pacheco I (2021) Nanoparticles as novel elicitors to improve bioactive compounds in plants. Agriculture 11: 134. https://doi.org/10.3390/agriculture11020134
Rojas-Downing MM, Nejadhashemi AP, Harrigan T, Woznicki SA (2017) Climate change and livestock: Impacts, adaptation, and mitigation. Climate Risk Management 16: 145-163. https://doi.org/10.1016/j.crm.2017.02.001
Sariñana-Navarrete MDLA, Morelos-Moreno Á, Sánchez E, Cadenas-Pliego G, Benavides-Mendoza A, Preciado-Rangel P (2023) Selenium nanoparticles improve quality, bioactive compounds and enzymatic activity in jalapeño pepper fruits. Agronomy 13: 652. https://doi.org/10.3390/agronomy13030652
SIAP (2023) Anuario estadístico de la producción agrícola. Gobierno de México. Servicio de Información Agroalimentaria y Pesquera. https://www.gob.mx/siap. Fecha de consulta: 20 de octubre de 2025.
Shang Y, Hasan MK, Ahammed GJ, Li M, Yin H, Zhou J (2021) Applications of nanotechnology in plant growth and crop protection: A review. Molecules 26(7): 1797. https://doi.org/10.3390/molecules26071797
Shen M, Liu W, Zeb A, Lian J, Wu J, Lin M (2022) Bioaccumulation and phytotoxicity of ZnO nanoparticles in soil-grown Brassica chinensis L. and potential risks. Journal of Environmental Management 306: 114454. https://doi.org/10.1016/j.jenvman.2022.114454
Steiner AA (1961) A universal method for preparing nutrient solutions of a certain desired composition. Plant and Soil 15: 134-154. https://doi.org/10.1007/BF01347224
Tan M, Yolcu H (2021) Current status of forage crops cultivation and strategies for the future in Turkey: A review. Journal of Agricultural Sciences 27: 114-121. https://doi.org/10.15832/ankutbd.903732
Tiwari K, Kumar Y, Singh T, Nayak R (2022) Nano technology based P fertilizers for higher efficiency and agriculture sustainability. Annals of Plant and Soil Research 24: 198-207. https://doi.org/10.47815/apsr.2022.10149
Van Soest PJ, Wine RH (1967) Use of detergents in the analysis of fibrous feeds. IV. Determination of plant cell-wall constituents. Journal of the Association of Official Analytical Chemists 50: 50-55. https://doi.org/10.1093/jaoac/50.1.50
Vázquez-Núñez E (2023) Uso de nanomateriales en la agricultura y sus implicaciones ecológicas y ambientales. Mundo Nano. Revista Interdisciplinaria en Nanociencias y Nanotecnología 16: 30. https://doi.org/10.22201/ceiich.24485691e.2023.30.69704
Wu H, Tong J, Jiang X, Wang J, Zhang H, Luo Y (2024) More effective than direct contact: Nano hydroxyapatite pre-treatment regulates the growth and Cd uptake of rice (Oryza sativa L.) seedlings. Journal of Hazardous Materials 463: 132889. https://doi.org/10.1016/j.jhazmat.2023.132889
Yadav A, Yadav K, Ahmad R, Abd-Elsalam KA (2023) Emerging frontiers in nanotechnology for precision agriculture: Advancements, hurdles and prospects. Agrochemicals 2: 220-256. https://doi.org/10.3390/agrochemicals2020016
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