Evaluation of the readiness of Genipa americana for in vitro gas production

Authors

DOI:

https://doi.org/10.19136/era.a11nIV.4035

Keywords:

arboreal, biogas, dry matter degradation, fruit, leaves

Abstract

The protocols of the in vitro gas production technique are intended to be standardized when evaluating tree species for feeding ruminants. The objective was to determine the effect of sample arrangement in the biodigester, as well as to characterize in vitro mature and immature leaves and fruits of Genipa americana. In a vial, the sample was placed directly or in a poly-silk bag; 40 mL of anaerobic culture medium, under CO2 flow and 10 mL of fresh rumen fluid as inoculum. The vials were incubated 72 h at 39 °C. Biogas production was measured at 2, 4, 6, 8, 10, 10, 12, 24, 48, and 72 h and dry matter degradation (DMD) at 72 h. The statistical analysis was a completely randomized design with a 3 x 2 factorial arrangement, with type of sample (leaf, immature and mature fruit) and disposition (direct or bagged) as factors. Biogas production showed no interaction between factors (P > 0.05); but DMD showed interaction (P ≤ 0.05). Biogas production showed no interaction between factors (P > 0.05); but DMD showed interaction (P ≤ 0.05). Biogas production did not show differences in sample arrangement (P > 0.05), except for 2 h (P ≤ 0.05). Leaves presented lower cumulative biogas production (P ≤ 0.05). DMD was different in the arrangement of mature and immature fruits (P ≤ 0.05), while in leaves there were no differences by arrangement (P ≤ 0.05). Sample arrangement affected the values of DMD and biogas production at 2 h in fruits.

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Author Biographies

  • Jerónimo Herrera-Pérez, Universidad Autónoma de Guerrero

    Universidad Autónoma de Guerrero, Facultad de Medicina Veterinaria y Zootecnia No. 2. Carretera federal Acapulco-Pinotepa Nacional Km 197, CP. 41940. Cuajinicuilapa, Guerrero, México

  • Paulino Sanchez Santillan, Universidad Autónoma de Guerrero

    Doctor en Ciencias en el área de Microbiología Ruminal, Profesor Investigador de Tiempo Completo Titular A, Facultad de Medicina Veterinaria y Zootecnia No. 2, Universidad Autonoma de GUerrero, Cuajinicuilapa, Guerrrero. Integrante del Sistema Nacional de Investigación nivel candidato, Integrante del perfil deseable de la Secretaria de Educación Publica. Coordinador del Cuerpo Academico "Producción sustentable de Rumiantes en el Trópico" UAGro-CA-183

  • Álvaro Lorenzo-Rojas , Universidad Autónoma de Guerrero

    Universidad Autónoma de Guerrero, Facultad de Medicina Veterinaria y Zootecnia No. 2. Carretera federal Acapulco-Pinotepa Nacional Km 197, CP. 41940. Cuajinicuilapa, Guerrero, México

  • Isaac Almaraz-Buendía, Universidad Autónoma del Estado de Hidalgo

    Universidad Autónoma del Estado de Hidalgo, Instituto de Ciencias Agropecuarias. Av. Universidad km. 1, CP. 43600, Tulancingo, Hidalgo, México

  • Maria Bottini-Luzardo , Universidad Autónoma de Guerrero

    Universidad Autónoma de Guerrero, Facultad de Medicina Veterinaria y Zootecnia No. 2. Carretera federal Acapulco-Pinotepa Nacional Km 197, CP. 41940. Cuajinicuilapa, Guerrero, México.

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Published

2024-12-13

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SCIENTIFIC ARTICLE

How to Cite

Herrera-Pérez, J., Sanchez Santillan, P., Lorenzo-Rojas , Álvaro, Almaraz-Buendía, I., & Bottini-Luzardo , M. (2024). Evaluation of the readiness of Genipa americana for in vitro gas production. Ecosistemas Y Recursos Agropecuarios, 11(IV). https://doi.org/10.19136/era.a11nIV.4035

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