Effect of conjugated linoleic acid on oocyte quality in female cd-1 mice
DOI:
https://doi.org/10.19136/era.a7n3.2666Keywords:
Ácidos grasos poliinsaturados, concentración de lípidos, calidad ovocitaria, ácido linoleico conjugado.Abstract
The objective of the present study was to evaluate the effect of conjugated linoleic acid on the quality and accumulation of lipids in oocytes of mice of the CD-1 strain. The females were distributed in two groups: control (TCON: n: 5) and conjugated linoleic acid (TCLA: n: 5), they were fed during 21 days. 53 h after treatment were sacrificed for the recovery of the oocytes, which were stained with Nile Red and observed under fluorescence. The TCLA group presented 25% more recovered oocytes compared to TCON, with 63% of oocytes of excellent and good quality (p ≤ 0.05), the lipid concentration in the TCLA group was lower (145.8 ± 17.8) compared to TCON (246 ± 13.1) (p < 0.05). The addition of conjugated linoleic acid in the diet of female CD-1 mice increases the number and quality of oocytes and decreases the accumulation of lipids in them.
Downloads
References
Aardema H, (2014) Impact of free fatty acid composition on oocyte developmental competence in dairy cows. Faculty of Veterinary Medicine 94: 618-637.
Aardema H, Lolicato F, Van de Lest CH, Brouwers JF, Vaandrager AB, Van Tol HT, et al. (2013) Bovine cumulus cells protect maturing oocytes from increased fatty acid levels by massive intracellular lipid storage. Biology of Reproduction 6: 1–15.
Al Darwich A; Perreau CP; Petit MH; Papillier P; Dupont J; Guillaume D; et al. (2010). Effectof PUFA on embryo cryoresistance, gene expression and AMPKα phosphorylation in IVF derived bovine embryos. Prostaglandins & Other Lipid Mediators 93:30-36.
Ashwell MS, Ceddia RP, House RL, Cassady JP, Eisen EJ, Eling TE. et al. (2010). “Trans-10, cis12-conjugated linoleic acid alters hepatic gene expression in a polygenic obese line of mice displaying hepatic lipidosis,” The Journal of Nutritional Biochemistry. 21: 848–855.
Batista RI, Raposo NR, Campos-Junior PH, Pereira MM, Camargo LS, Carvalho BC, et al. (2014) Trans-10, cis-12 conjugated linoleic acid reduces neutral lipid content and may affect cryotolerance of in vitro-produced crossbred bovine embryos. Journal of Animal Science and Biotechnology. 5: 3-8.
Borges DE and Vireque AA. (2019). Updating the Impact of Lipid Metabolism Modulation and Lipidomic Profiling on Oocyte Cryopreservation. European Medical Journal. 4: 79-87.
Bradley J and Swann K (2019). Mitochondria and lipid metabolism in mammalian oocytes and early embryos. The International Journal of Developmental Biology. 63: 93-103
Chung S, Brown JM, Sandberg MB, McIntosh M. (2005) Trans-10, cis-12 CLA increases adipocyte lipolysis and alters lipid droplet-associated proteins: role of mTOR and ERK signaling,” The Journal of Lipid Research. 46: 885– 895.
Dunning KR, Russell DL, Robker, RL. (2014). Lipids and oocyte developmental competence: the role of fatty acids and b-oxidation. Reproduction 148. doi:10.1530/REP-13-0251
Jenkins TC, Wallace RJ, Moate PJ, Mosley EE. (2008) Recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem. Animal Science 86: 399-412.
Jin JX, Lee S, Taweechaipaisankul A, Kim GA, Lee BC. (2017). Melatonin regulates lipid metabolism in porcine oocytes. Journal of Pineal Research. 62. doi:10.1111/JPI.12388.
Jungheim ES, Macones GA, Odem RR, Patterson BW, Lanzendorf SE, Ratts VS, et al. (2011) Associations between free fatty acids, cumulus oocyte complex morphology and ovarian function during in vitro fertilization. Fertility and Sterility 95: 1970-1974.
Kim JY, Kinoshita M, Ohnishi M, Fukui Y. (2001) Lipid and fatty acid analysis of fresh and frozen-thawed 1µ Mature and in vitro matured bovine oocytes. Reproduction Fertility 122: 131-138.
Lapa M, Marquez CC, Alves SP, Vasques MI, Baptista MC, Carvalhais I, et al. (2011) Effect of trans-10 cis-12 conjugated linoleic acid on Bovine Oocyte Competence and Fatty Acid Composition. Reproduction in domestic animals 46: 906-910.
Leão BC, Rocha-Frigoni NA, Cabral EC, Coelho M B, Ferreira C R, Eberlin M N. (2015) Improved embryonic cryosurvival observed after in vitro supplementation with conjugated linoleic acid is related to changes in the membrane lipid profile. Theriogenology. 84: 127-136.
Leão BCS, Rocha-Frigoni NAS, Cabral EC, Franco MF, Ferreira CR, Eberlin MN, et al (2014) Membrane lipid profile monitored by mass spectrometry detected differences between fresh and vitrified in vitro-produced bovine embryos. Zygote 1–10. doi.org/10.1017/S0967199414000380.
Leão BCS, Rocha-Frigoni NAS, Nogueira É, Cabral EC, Ferreira C R, Marcos N, et al. (2017) Membrane lipid profile of in vitro-produced embryos is affected by vitrification but not by long-term dietary supplementation of polyunsaturated fatty acids for oocyte donor beef heifers. Reproduction Fertility and Development. 29:1217-1230.
Leroy MR, Hoeck VV, Clemente M, Rizos D, Gutierrez AA, Soom VA, et al. (2010) The effect of nutritionally induced hyperlipidaemia on in vitro bovine embryo quality. Human Reproduction 25(3): 768-778.
Marei W, Wathe D, Fouladi-Nashta A. (2010) Impact of linoleic acid on bovine oocyte maturation and embryo development. Reproduction fertility 139: 979-988.
Matos JE, Marques CC, Moura TF, Baptista CM, Horta EMA, Soveral G, et al. (2015) Conjugated linoleic acid improves oocyte cryosurvival through modulation of the cryoprotectants influx rate. Reproductive Biology and Endocrinology. doi:10.1186/s12958-015-0059-3.
McEvoy TG, Onal AG, Speake BK, Robinson JJ. (2012). Impact of contrasting fish oil concentrations in the diet on ovine embryo development in vivo and of corresponding diet-specific derivative sera during in vitro culture. Journal of Animal and Feed Sciences 21: 31-48.
Meza-Villalvazo, VM, Magaña SH, Sandoval CC, Morales MR, Chay-Canul A, Trejo CA. (2013). Efecto de los ácidos grasos poliinsaturados sobre población folicular y calidad ovocitaria en ovejas Pelibuey. Universidad y Ciencia. 29: 255-261.
Meza-Villalvazo, VM, Trejo CA, Magaña SH, Sandoval CC, Chay-Canul A, Cavazos GA. et al. (2014). Perfil metabólico de isómeros de Ácido Linoleico Conjugado y calidad de ovocitos en ovejas de pelo. Nova Scientia, 12 (2): 287-303.
Paczkowski M, Silva E, Schoolcraft W, Krisher R. (2013) Comparative Importance of Fatty Acid Beta-Oxidation to Nuclear Maturation, Gene Expression, and Glucose Metabolism in Mouse, Bovine, and Porcine Cumulus Oocyte Complexes. Biology of Reproduction 88: 1–11.
Park SE; Son W Y; Lee KA; Ko JJ, Cha KY. (1997). Chromosome and spindle configurations of human oocytes matured in vitro after cryopreservation at the germinal vesicle stage. Fertility and Sterility 68: 920-926.
Patricio P, Tucker MJ, Guelman V. (2003) Atlas de reproducción asistida. Editorial McGraw Hill, México.
Pena CB, de Queirós CF, Detoni D, Borges FR, Burla DAJ. (2019). Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content. Revista Brasileira de Zootecnia. 48:e20180322. doi.org/10.1590/rbz4820180322.
Pereira RM, Baptista MC, Vasques MI, Horta AE, Portuga PV, Bessa RJ, et al. (2007) Cryosurvival of bovine blastocysts is enhanced by culture with trans-10 cis-12 conjugated linoleic acid (t10, c12 CLA). Animal Reproduction Science 98: 293– 301.
Prates EG, Alves SP, Marques CC, Baptista MC, Horta AEM, Bessa RJB. et al. (2013) Fatty acid composition of porcine cumulus oocyte complexes (COC) during maturation: effect of the lipid modulatorstrans-10,cis-12 conjugated linoleic acid (t10, c12 CLA) and forskolin, In Vitro Cellular & Developmental Biology-Animal. 49: 335–345.
Prates EG, Nunes J, Pereira R. (2014) A Role of Lipid Metabolism during Cumulus Oocyte Complex Maturation: Impact of Lipid Modulators to Improve Embryo Production. Mediators of inflammation. doi.org/10.1155/2014/692067.
Sudano MJ, Santos VG,
Downloads
Published
Issue
Section
License
Aviso de copyright
Los autores que se envían a esta revista aceptan los siguientes términos:
una. Los autores conservan los derechos de autor y garantizan a la revista el derecho a ser la primera publicación del trabajo con una licencia de atribución de Creative Commons que permite a otros compartir el trabajo con un reconocimiento de la autoría del trabajo y la publicación inicial en esta revista.
B. Los autores pueden establecer acuerdos complementarios separados para la distribución no exclusiva de la versión del trabajo publicado en la revista (por ejemplo, en un repositorio institucional o publicarlo en un libro), con un reconocimiento de su publicación inicial en esta revista.
C. Se permite y se anima a los autores a difundir su trabajo electrónicamente (por ejemplo, en repositorios institucionales o en su propio sitio web) antes y durante el proceso de envío, ya que puede conducir a intercambios productivos, así como a una cita más temprana y más extensa del trabajo publicado. (Consulte El efecto del acceso abierto).