Introgression of transgenic events and accompanying sequences into Mexican maize varieties

Authors

  • Jessica Nallely Siller-García Food Research Department. School of Chemistry. Universidad Autónoma de Coahuila.
  • Miguel Ángel Cruz-González Food Research Department. School of Chemistry. Universidad Autónoma de Coahuila.
  • Francisco Castillo-Reyes National Research Institute of Forestry, livestock and Agriculture. Saltillo Experimental Station
  • Sergio Alfredo Rodríguez-Herrera Plant Breeding Department, Universidad Autónoma Agraria Antonio Narro3Plant Breeding Department, Universidad Autónoma Agraria Antonio Narro
  • Jesús Antonio Morlett-Chávez Food Research Department. School of Chemistry. Universidad Autónoma de Coahuila
  • Cristóbal N. Aguilar Food Research Department. School of Chemistry. Universidad Autónoma de Coahuila
  • Raúl Rodríguez-Herrera Food Research Department. School of Chemistry. Universidad Autónoma de Coahuila

DOI:

https://doi.org/10.19136/era.a10n2.3538

Keywords:

Razas mexicanas, contaminación genética, PCR, eventos transgénicos, Cry1Ab, ntpII

Abstract

Representative seed samples of 215 of maize varieties from four
(Jalisco, Michoacán, Oaxaca and Puebla) Mexican States were collected. The total genotypes collected in each State were 53, 96, 46 and 20 samples, respectively. Each seed collection was geo-referenced, then seed was planted and leaf tissue was used for DNA isolation, after that a PCR analysis was performed to determine the presence or absence of eight accompanying sequences and nine transgenic
events in the 215 maize genotypes. Results showed introgression of accompanying sequences and transgenic events (cry1Ab) into some maize varieties. The major proportion of cry1Ab residues was observed in maize samples from Puebla (70%)
followed by that displayed in the Oaxaca samples (52%), while Jalisco samples showed 15 and 7.5% of the cry1Ab gene and ntpII accompanying sequence, respectively, and Michoacán samples exhibited 44 and 17.7% for cry1Ab gene and the accompanying sequence, respectively. These results supported for applicate corrective measures for to prevent the genetic contamination of Mexican maize varieties and to clean up out-of-type phenotypes that could be sources of contamination of transgenic genes within maize populations contaminated with transgenes.

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References

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Published

2023-08-15

How to Cite

Siller-García, J. N., Cruz-González, M. Ángel, Castillo-Reyes, F., Rodríguez-Herrera, S. A., Morlett-Chávez, J. A., Aguilar, C. N., & Rodríguez-Herrera, R. (2023). Introgression of transgenic events and accompanying sequences into Mexican maize varieties. Ecosistemas Y Recursos Agropecuarios, 10(2). https://doi.org/10.19136/era.a10n2.3538

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Section

SCIENTIFIC ARTICLE

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