Actividad antifúngica de antioxidantes derivados de cuatro cultivares de Capsicum spp. contra hongos fitopatógenos

Nancy Raquel López Muñoz, Mirella Romero-Bastidas, Pablo Misael Arce Amezquita, José Saul Hernandez Rubio

Abstract


El cultivo de chile, además del uso culinario posee actividad antimicrobiana, debido a la acción de antioxidantes. Por lo que es de interés en la producción agrícola, como alternativa en el manejo de enfermedades en plantas. El objetivo del estudio fue evaluar la capacidad antioxidante de cuatro variedades de chile y su efecto antifúngico contra Fusarium andiyaziCochliobollusspp., ambos son patógenos severos en el cultivo de espárrago y albahaca. A partir de muestras de frutos de chile habanero, jalapeño, serrano y morrón, se obtuvieron extractos etanólicos, donde se determinó su capacidad antioxidante. La acción antifúngica, se evaluó mediante dos técnicas; la prueba de zona de inhibición en PDA y la germinación de esporas en caldo de papa-dextrosa. La actividad de los extractos se evaluó mediante la medición del halo de inhibición, a los cinco días después de la siembra y la germinación de esporas a través de la cuantificación de las mismas a las 48 h. Los resultados indican que el extracto de chile habanero presentó mayor capacidad antioxidante (11.9 mg g-1) y porcentaje de inhibición contra F. andiyaziCochliobollusspp., (55.61 y 82.29%), al tener respuesta similar al fungicida. La acción de inhibición de los patógenos en el resto de los extractos, varió entre éstos independiente de su capacidad antioxidante, donde posiblemente actúen otros compuestos. El extracto de chile habanero es eficaz para controlar Cochliobollusspp. y Fusarium andiyazi, y puede ser una alternativa para el control de estos fitopatógenos en la agricultura orgánica.


References


Aguiar AC., Pereira CJ, Fernández BG, Teixeira GH, Martínez J (2016) Comparative study of capsaicinoid composition in Capsicum peppers grown in Brazil. International Journal of Food Properties 19: 1292-1302. DOI: 10.1080/10942912.2015.1072210.

Akila R, Rajendran L, Harish S, Saveetha K, Raguchander T, Samiyappan R (2011) Combined application of botanical formulations and biocontrol agents for the management of Fusarium oxysporum f. sp. cubense (Foc) causing Fusarium wilt in banana. Biological Control 57: 175-183. https://doi.org/10.1016/j.biocontrol.2011.02.010.

Alam MA, Juraimi AS, Rafii MY, Hami AA, Aslani F, Alam MZ (2015) Effects of salinity and salinity-induced augmented bioactive compounds in purslane (Portulaca oleracea L.) for possible economical use. Food Chemistry 169: 439-447. DOI:10.1016/j.foodchem.2014.08.019.

Bacon K, Boyer R, Denbow C, O’Keefe S, Neilson A, Williams RC (2016) Evaluation of different solvents to extract antibacterial compounds from Jalapeno peppers. Food Science & Nutrition 5(3): 497-503. doi: 10.1002/fsn3.423.

Bertão MR, Moraes MC, Palmieri DA, Pereira SL, Gonçalves DRM (2016) Cytotoxicity, genotoxicity and antioxidant activity of extracts from Capsicum spp. Research Journal of Medicinal Plants 10: 265-275. DOI: 10.3923/rjmp.2016.265.275.

Broekaert WF, Terras FRG, Cammue BPA, Vanderleyden J (1990) An automated quantitative assay for fungal growth inhibition. Federation of European Microbiological Societies 69: 55-60. DOI: 10.1016/0378-1097(90)90412-J.

Castro-Concha LA, Canche-Chuc I, Miranda-Ham MDL, (2012) “Determination of antioxidants in fruit tissues from three accessions of habanero pepper (Capsicum chinense Jacq.),” Journal of the Mexican Chemical Society 56: 15-18.

Castro-Concha L, Tuyub-Che J., Moo-Mukul A, Vazquez-Flota F, Miranda-Ham M (2014) Antioxidant capacity and total phenolic content in fruit tissues accessions of Capsicum chinense jacq. (Habanero pepper) at different stages of ripening. The Scientific World Journal 2014: 1-5. http://dx.doi.org/10.1155/2014/809073.

Cerón CTG, Santiesteban-López NA, Acle MR (2016). Combinación de dos extractos de Capsicum: composición y actividad antimicrobiana. Revista Iberoamericana de las Ciencias Biológicas y Agropecuarias 5: 1-19.

Corato U, Maccioni O, Trupo M, Di Sanzo G (2010) Use of essential oil of Laurus nobilis obtained by means of a supercritical carbon dioxide technique against post harvest spoilage fungi. Crop Protection 29: 142-147. https://doi.org/10.1016/j.cropro.2009.10.012.

Cruz-Rodríguez RI, Meza-Gordillo R, Rodríguez-Mendiola MA, Arias-Castro C, Mancilla-Margalli NA, Ávila-Miranda ME, Culebro-Ricaldi JM, Gutiérrez-Miceli FA, Ruiz-Valdiviezo VM, Ayora-Talavera TR (2017) Antifungal activity of Crotalaria longirostrata Hook. & Arn. extracts against phytopathogen fungi from maize. Gayana botanica 74: 167-175. ISSN 0016-5301.

Dissanayake MLMC (2014) Inhibitory effect of selected medicinal plant extracts on phytopathogenic fungus Fusarium oxysporum. Annual Research & Review in Biology 4: 133-142. http://dx.doi.org/10.9734/ARRB/2014/5777.

Dorantes L, Colmenero R, Hernández H, Mota L, Jaramillo ME, Fernández E, Solano C (2000) Inhibition of growth of some food-borne pathogenic bacteria by Capsicum annum extracts. International Journal of Food Microbiology 57: 125-128. https://doi.org/10.1016/S0168-1605(00)00216-6.

Govindappa M, Bharath N., Shruthi HB, Sadananda TS, Sharanappa P (2011) Antimicrobial, antioxidant and in vitro anti-inflammatory activity and phytochemical screening of Crotalaria pallida Aiton. African Journal of Pharmacy and Pharmacology 5: 2359-2371. DOI: 10.5897/AJPP11.038.

Gurnani N, Gupta M, Mehta D, Kumar MB (2016) Chemical composition, total phenolic and flavonoid contends, and in vitro antimicrobial and antioxidant activities of crude extratc from red chilli seeds (Capsicum frutescens L.). Journal of Taibah University for Science 10; 462-470. DOI: 10.1016/j.jtusci.2015.06.011.

Guzmán de Peña D, Aguirre J, Ruiz HJ (1998). Correlation between the regulation of sterigmatocystin biosynthesis and asexual and sexual sporulation in Emericella nidulans. Antonnie Van Leeuwenhoek 73: 199-205.

Hadi M, Kashefi B, Sobhanipur A, Rezaarabsorkhi (2013) Study on effect of some medicinal plant extracts on growth and spore germination of Fusarium oxysporum schlecht. In vitro. Ed. American-Eurasian Journal of Agricultural and Environmental Science 13: 581-588.

Hernández J, Dorantes L, Jaramillo ME, Ramiro C (2005) Determinación de algunas variedades de Capsicum y su relación con carotenoides totales. In: Memoria de la Segunda Convención Mundial del Chile. Zacatecas, Zac. 53-57.

Hernández-Ortega A, Luca F, Ferreira P, Medina M, Guallar V (2011) Modulating O2 reactivity in a fungal flavoenzyme: involvement of aryl-alcohol oxidase phe-501 contiguous to catalytic histidine. Journal of Biological Chemistry 286: 41105-41114. DOI 10.1074/jbc.M111.282467.

Iqbal E, Salim KA, Lim LBL (2015) Phytochemical screening, total phenolics and antioxidant activities of bark and leaf extracts of Goniothalamus velutinus (Airy Shaw) from Brunei Darussalam. Journal of King Saud University-Science 27: 224-232. https://doi.org/10.1016/j.jksus.2015.02.003.

Kabara JJ, Marshall (2005) ”Medium-chain fatty acids and esters” En: Davidson P.M, J.N Sofos, and A.L Branen (eds.) Antimicrobials in food. Marcel Dekker. New York. 327-360. DOI:10.1201/9781420028737.ch11.

Lanciotti R, Gianotti A, Patrignani F, Belletti N, Guerzoni ME, Gardini F (2014). Use of natural aroma compounds to improve shelflife and safety of minimally processed fruits. Food Science & Technology 15: 201-208. https://doi.org/10.1016/j.tifs.2003.10.004.

Lee Y, Howard LR, Villalón B (1995) Flavonoids and antioxidant activity of fresh pepper (Capsicum annuum) cultivars. Journal of Food Science 60: 473-476. https://doi.org/10.1111/j.1365-2621.1995.tb09806.x.

Loizzo MR, Pugliese A, Bonesi M, Menichini F, Tundis R (2015) Evaluation of chemical profile and antioxidant activity of twenty cultivars from Capsicum annum, Capsicum baccatum, Capsicum chacoense and Capsicum chinense: A comparison between fresh and processed peppers. LWT-Food Science and Technology 64: 623-631. https://doi.org/10.1016/j.lwt.2015.06.042.

López G, Hermann M (2004) El cultivo del ulluco en la sierra central del Perú. CIP.pais. 51p.

Lu A, Onoja U, Onyeke CC, Nweze El (2017) Antimicrobial activities of four varieties of Capsicum annuum fruits cultivated in Southeast Nigeria against multidrug-resistant and susceptible organisms. Journal of Basic Pharmacology and Toxicology 1: 21-26. http://www.scigreen.com/index.php/JBPT/article/view/21.

Mahlo SM, McGaw LJ, Eloff JN (2010) Antifungal activity of leaf extracts from South African trees against plant pathogens, Crop Protection 29: 1529-1533. https://doi.org/10.1016/j.cropro.2010.08.015.

Materska M (2014) Bioactive phenolics of fresh and freeze-dried sweet and semi-spicy pepper fruits (Capsicum annuum L.). Journal of Functional Foods 7: 269-277. https://doi.org/10.1016/j.jff.2014.02.002.

Medina-Juárez LA, Molina-Quijada DMA, Del Toro SCL, González-Aguilar GA, Gámez-Meza N (2012) Actioxidant activity of peppers (Capsicum annuum L) extracts and characterization of their phenolic constituents. Interciencia. 37: 588-593.

GraphPad Prism v.7. Paquete de Diseños Experimentales

Ornelas-Paz JJ, Martínez BJM, Ruíz CS, Santana RV, Ibarra JV, Olivas GI, Pérez MJD (2010) Effect of cooking on the capsaicinoids and phenolics content of Mexican peppers. Food Chemistry 119: 1619-1625. https://doi.org/10.1016/j.foodchem.2009.09.054.

Pang CH, Wang BS (2010) Ascorbate-glutathione pathway and stress tolerance in plants. in: Chan MT, Umar S, Naser A, Stress. The International Journal on the Biology of Stress 91-113.

Ríos J, Recio M, Villar A (1988) Screening methods for natural products with antimicrobial activity. Journal of Ethnopharmacology 23: 127-149. https://doi.org/10.1016/0378-8741(88)90001-3.

Rodríguez MA, Troncoso RR, Sánchez EA, González MD, Ruiz SE, Zamora BR, et al. (2015) Efecto antifúngico de extractos fenólicos y de carotenoides de chiltepín (Capsicum annum var. glabriusculum) en Alternaria alternata y Fusarium oxysporum. Revista Argentina de Microbiología 47: 72-77. DOI: 10.1016/j.ram.2014.12.005.

Shahidi F (2000) Antioxidant factors in plant foods and selected oil seeds. BioFactors 13: 179-185.

Srinivasan S, Sarada DVL (2012) Antifungal activity of phenyl derivative of pyranocoumarin from Psoralea corylifolia L. seeds by inhibition of acetylation activity of trichothecene 3-O-acetyltransfarase (Tri101). Journal of Biomedicine and Biotechnology 2012: 1-8. http://dx.doi.org/10.1155/2012/310850.

Tag HM, Kelany OE, Tantawy HM, Fahmy AA (2014) Potential anti-inflammatory effect of lemon and hot pepper extracts on adjuvant-induced arthritis in mice. Journal of Basic and Applied Zoology 67:149-157. https://doi.org/10.1016/j.jobaz.2014.01.003.

Treutter D (2005) Significance of flavonoids in plant resistance and enhancement of their biosíntesis. Journal of Plant Biology 7: 581-591. DOI: 10.1055/s-2005-873009.

Vashisth T, Singh RK, Pegg RB (2011) Effects of drying on the phenolics content and antioxidant activity of Muscadine pomace. LWT - Food in Science & Technology 44: 1649-1657. https://doi.org/10.1016/j.lwt.2011.02.011.

Wilson C, Solar J, El Ghaouth A, Wisniewski M (1997) Rapid Evaluation of Plant Extract and Essential Oils for Antifungal Activity Against Botrytis cinérea. Plant Disease 81: 204-210. https://doi.org/10.1094/PDIS.1997.81.

Yamaguchi T, Takamura H, Matoba T, Terao J (1998) HPLC Method for Evaluation of the Free Radical-scavenging Activity of Foods by Using 1,1-Diphenyl-2-picrylhydrazyl. Bioscience, Biotechnology and Biochemistry 62: 1201-1204. DOI: 10.1271/bbb.62.1201.




DOI: http://dx.doi.org/10.19136/era.a6n18.2174

ECOSISTEMAS Y RECURSOS AGROPECUARIOS(ECOSYSTEMS AND AGRICULTURAL RESOURCES), Year 6, Issue 16, January-April 2019, is a triannual journal edited, published and distributed by the Universidad Juárez Autónoma de Tabasco, Av. Universidad s/n, Zona de la Cultura, Col. Magisterial, Villahermosa, Centro, Tabasco, CP. 86040, Tel (993) 358 15 00, www.ujat.mx, era@ujat.mx., era@ujat.mx. Editor-in-chief: Efraín de la Cruz Lázaro. Copyright No. 04-2013-120514213600-203, ISSN: 2007-901X, both granted by the Instituto Nacional del Derecho de Autor (National Institute of Copyright), with certificate of title and content No. 16540 granted by the Secretaría de Gobernación(Ministry of the Interior). Individual responsible for the last update of this issue was journal Editorial Assistant Lic. Misael Hernández Martínez, Av. Universidad s/n, Zona de la Cultura, Col. Magisterial, Vhsa, Centro, Tabasco, Mex. C.P. 86040; date of last modification, December 31, 2018.

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