Selection of Streptomyces sp. to cope with biotic and abiotic stress in Nicotiana tabacum L.
DOI:
https://doi.org/10.19136/era.a13n3.4984Keywords:
Actinomycetes, tobacco, salinity and drought stress, antagonistic activityAbstract
Climate change intensifies the effects of biotic and abiotic stress on plant growth and productivity; drought and salinity are two relevant abiotic inducers that affect agricultural productivity. The antagonistic activity of Streptomyces sp. (strains B8, EA2, CB14, CD24) against phytopathogenic fungi isolated from tobacco plants (Nicotiana tabacum L.) was evaluated, as well as its in vitro tolerance to different concentrations of sodium chloride (NaCl), pH, and polyethylene glycol-600 (PEG), and its effect on the germination of N. tabacum L. seeds. The results of the in vitro bioassay showed a broad antagonistic spectrum against Fusarium spp. and Phytophthora nicotianae, which affect tobacco crops. All actinomycetes strains grew under different stress conditions (NaCl, pH, and PEG). The results obtained regarding tobacco seed germination parameters indicate that the best treatment was Streptomyces sp. CD24, as it stimulated the germination rate, germination energy, and germination speed index. Streptomyces spp. strains constitute a novel alternative to maintain and increase the growth and productivity of tobacco crops, in order to alleviate and mitigate the effects of salinity and drought stress.
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References
Akond MA, Jahan MN, Sultana N, Rahman F (2016) Effect of temperature, pH and NaCl on the isolates of actinomycetes from straw and compost samples from Savar, Dhaka, Bangladesh. American Journal of Microbiology and Immunology 1(2): 10-5.
Alam K, Mazumder A, Sikdar S, Zhao YM, Hao J, Song C, Wang Y, Sarkar R, Islam S, Zhang Y, Li A (2022) Streptomyces: The biofactory of secondary metabolites. Frontiers in Microbiology 13: 968053. https://doi.org/10.3389/fmicb.2022.968053
Ashraf MY, Azmi AR, Khan AH, Naqvi SSM (1995) Alpha-amylase and protease activities of wheat seedling grown under water stress conditions. Biological Sciences – PJSIR 38(11-12): 430-434.
Bacchetta G, Bueno Sánchez A, Fenu G, Jiménez-Alfaro B, Mattana E, Piotto B, Virevaire M (2008) Conservación ex situ de plantas silvestres. Ed. Jardín Botánico Atlántico, Principado de Asturias – La Caixa. 375p.
Basu S, Roychoudhury A, Saha PP, Sengupta DN (2010) Comparative analysis of some biochemical responses of three indica rice varieties during polyethylene glycol-mediated water stress exhibits distinct varietal differences. Acta Physiologiae Plantarum 32: 551-563. https://doi.org/10.1007/s11738-009-0432-y
Bhatt RM, Selvakumar G, Upreti KK, Boregowda P (2015) Effect of biopriming with Enterobacter strains on seed germination and seedling growth of tomato (Solanum lycopersicum L.) under osmotic stress. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences 85(1): 63-69. https://doi.org/10.1007/s40011-014-0333-8
Boruta T (2021) A bioprocess perspective on the production of secondary metabolites by Streptomyces in submerged co-cultures. World Journal of Microbiology and Biotechnology 37: 171. https://doi.org/10.1007/s11274-021-03141-z
Budzianowski J (2015) Tobacco against ebola virus disease. Przeglad Lekarski 72(10): 567–571.
Calero A, Quintero E, Pérez Y, Oliviera D, Peña K (2019) Influencia de dos bioestimulantes en el comportamiento agrícola del cultivo del tabaco (Nicotiana tabacum L.). Revista de la Facultad de Ciencias 8(1): 31-44. https://doi.org/10.15446/rev.fac.cienc.v8n1.73546
Chaudhry S, Sidhu G (2022) Climate change regulated abiotic stress mechanisms in plants: a comprehensive review. Plant Cell Reports 41: 1–31. https://doi.org/10.1007/s00299-021-02759-5
Chávez-González M, Arredondo Valdés R, Ascacio Valdés JA, Govea Salas M, Ilyina A (2021) Application of Streptomyces antimicrobial compounds for the control of phytopathogens. Frontiers in Sustainable Food Systems 5: 696518. https://doi.org/10.3389/fsufs.2021.696518
Chen Y, Zhou D, Qi D, Gao Z, Xie J, Luo Y (2018) Growth promotion and disease suppression ability of a Streptomyces sp. CB-75 from banana rhizosphere soil. Frontiers in Microbiology 8: 2704. https://doi.org/10.3389/fmicb.2017.02704
Cinkocki R, Lipkova N, Javorekova S, Petrova J, Makova J, Medo J, Ducsay L (2021) The impact of growth-promoting streptomycetes isolated from rhizosphere and bulk soil on oilseed rape (Brassica napus L.) growth parameters. Sustainability 13(10): 5704. https://doi.org/10.3390/su13105704
Collado R, Veitía N, Bermúdez-Caraballoso I, García LR, Torres D, Romero C, Rodríguez Lorenzo JL, Angenon G (2013) Efficient in vitro plant regeneration via indirect organogenesis for different common bean cultivars. Scientia Horticulturae 153: 109-116. https://doi.org/10.1016/j.scienta.2013.02.007
Compant S, Samad A, Fais H, Sessitsch A (2019) Review on the plant microbiome: Ecology, functions, and emerging trends in microbial application. Journal of Advanced Research 19: 29-37. https://doi.org/10.1016/j.jare.2019.03.004
Dalal A, Bourstein R, Haish N, Shenhar I, Wallach R, Moshelion M (2019) Dynamic physiological phenotyping of drought-stressed pepper plants treated with ‘productivityenhancing’ and ‘survivability-enhancing’ biostimulants. Frontiers in Plant Science 905(10): 2-3. https://doi.org/10.3389/fpls.2019.00905
De-Souza R, Ambrosini A, Passaglia LMP (2015) Plant growth promoting bacteria as inoculants in agricultural soils. Genetics and Molecular Biology 38: 401-419. https://doi.org/10.1590/S1415-475738420150053
Díaz-Díaz M, Bernal-Cabrera A, Trapero A, Jiménez-González A, Medina-Marrero R, Cupull-Santana RD, Águila-Jiménez E, Agustí-Brisach C (2023) Biocontrol of root rot complex disease of Phaseolus vulgaris by Streptomyces sp. strains in the field. Crop Protection 165: 106164. https://doi.org/10.1016/j.cropro.2022.106164
Díaz-Díaz M, Bernal-Cabrera A, Trapero A, Medina-Marrero R, Sifontes-Rodríguez S, Cupull-Santana RD, García-Bernal M, Agustí-Brisach C (2022) Characterization of actinobacterial strains as potential biocontrol agents against Macrophomina phaseolina and Rhizoctonia solani, the main soil-borne pathogens of Phaseolus vulgaris in Cuba. Plants 11: 645. https://doi.org/10.3390/plants11050645
Donohue K, Rubio de Casas R, Burghardt L, Kovach K, Willis CG (2010) Germination, post germination adaptation, and species ecological ranges. Annual review of ecology, evolution, and systematics 41(1): 293-319. https://doi.org/ 10.1146/annurev-ecolsys-102209-144715
Fatema, Khan ZH, Khan, ND, Mular SM (2017) Determination of amylase activity from germinated Syzygium cumini seed (jamun). International Journal of Applied Research 3(1): 573-575.
Fatmawati U, Meryandini A, Nawangsih AA, Wahyudi AT (2019) Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth. Biodiversitas Journal of Biological Diversity 20: 2970–2977. https://doi.org/10.13057/biodiv/d201027
García-Bernal M, Campa-Córdova ÁI, Saucedo PE, González MC, Marrero RM, Mazón-Suástegui JM (2015) Isolation and in vitro selection of actinomycetes strains as potential probiotics for aquaculture. Veterinary world 8(2): 170 https://doi.org/10.14202/vetworld.2015.170-176
García-Bernal M, Medina-Marrero R, Cuevas-Hernández L, Álvarez-Hernández U, Ojeda-Silvera CM, Batista-Sánchez D, Mazón-Suástegui JM (2025) Actinomicetos promueven crecimiento en plántulas de Nicotiana tabacum L. Terra Latinoamericana 43: 1-11. e1981. https://doi.org/10.28940/terra.v43i.1981
Hartmann H, Kester F (2001) Propagación de plantas, principios y prácticas. Editorial Continental. México. 760p.
ISTA (2010) Rules proposals for the International Rules for Seed Testing 2010 Edition. OM Rules Proposals for the International Rules for Seed Testing 2010 Edition.doc. Approved by ECOM Decision. No.498. International Seed Testing Association. Bassersdorf, Switzerland: ISTA.
Khamna S, Yokota A, Peberdy JF, Lumyong S (2010) Indole-3-acetic acid production by Streptomyces sp. isolated from some thai medicinal plant rhizosphere soils. Euro Asian Journal of Biological Science 4: 23-32. https://doi.org/10.5053/ejobios.2010.4.0.4
Krishnan A, Sampath-Kumar S (2015) Optimization of alpha amylase extracted from marine actinomycetes-Streptomyces gancidicus ASD-KT852565. International Research Journal of Pharmacy 6: 729-882 35. https://doi.org/10.7897/2230-8407.0610142
Kumar A, Gautam RD, Singh S, Chauhan R, Kumar A, Singh S (2022) Comparative study of the effects of different soluble salts on seed germination of wild marigold (Tagetes minuta L.). Journal of Applied Research on Medicinal and Aromatic Plants 31: 100421. https://doi.org/10.1016/j.jarmap.2022.100421
Le KD, Kim J, Nguyen HT, Yu NH, Park AR, Lee CW, Kim JC (2021) Streptomyces sp. JCK-6131 protects plants against bacterial and fungal diseases via two mechanisms. Frontiers in Plant Science 12: 726266. https://doi.org/10.3389/fpls.2021.726266
Liu D, Yan R, Fu Y, Wang X, Zhang J, Xiang W (2019) Antifungal, plant growth-promoting, and genomic properties of an endophytic actinobacterium Streptomyces sp. NEAU-S7GS2. Frontiers in Microbiology 10: 2077. https://doi.org/10.3389/fmicb.2019.02077
López-Reyes PK, De la Torre-Zavala S, Cortés-González MM, Galán-Wong LJ, Avilés-Arnaut H (2024) Biological control of Streptomyces sp. PR69 against Phytophthora capsici and its growth-promoting effects on plants. Horticulturae 10: 1365. https://doi.org/10.3390/horticulturae10121365
Ma Z, Bykova NV, Igamberdiev AU (2017) Cell signaling mechanisms and metabolic regulation of germination and dormancy in barley seeds. The Crop Journal 5(6): 459–77. https://doi.org/10.1016/j.cj.2017.08.007
Ma L, Zhang HY, Zhou XK, Yang CG, Zheng SC, Duo L, Mo MH (2018) Biological control tobacco bacterial wilt and black shank and root colonization by bioorganic fertilizer containing bacterium Pseudomonas aeruginosa NXHG29. Applied Soil Ecology 129: 136-144. https://doi.org/10.1016/j.apsoil.2018.05.011
Manullang W, Chuang H (2020) Streptomyces sp. mitigates abiotic stress response and promotes plant growth. Journal of Plant Protection Research 60(3): 263–274. https://doi.org/10.24425/jppr.2020.133955
Marasco R, Rolli E, Ettoumi B, Vigani G, Mapelli F, Borin S, Abou-Hadid AF, El-Behairy UA, Sorlini C, Cherif A, Zocchi G, Daffonchioet D (2012) A drought resistance promoting microbiome is selected by root system under desert farming. PloS One 7(10): e48479. https://doi.org/10.1371/journal.pone.0048479
Martínez-Viera R, Dibut A. (2012) Biofertilizantes bacterianos. Editorial científico técnica, La Habana, Cuba. 279p.
Meena DC, Birthal PS, Kumara TK (2025) Biostimulants for sustainable development of agriculture: a bibliometric content analysis. Discover Agriculture 3(1): 2. https://doi.org/10.1007/s44279-024-00149-5
Misk A, Franco C (2011) Biocontrol of chickpea root rot using endophytic actinobacteria. BioControl 56(5): 811-822. https://doi.org/10.1007/s10526-011-9352-z
Murugan L, Krishnan N, Venkataravanappa V, Saha S, Mishra AK, Sharma BK, Rai AB (2020) Molecular characterization and race identification of Fusarium oxysporum f. Sp. Lycopersici infecting tomato in India. 3 Biotech 10(11): 486. https://doi.org/10.1007/s13205-020-02475-z
Noe GB, Zedler JB (2000) Differential effects of four abiotic factors on the germination of salt marsh annuals. American Journal of Botany 87(11): 1679-1692. https://doi.org/10.2307/2656745
Ntemafack A, Ahmed S, Kumar A, Chouhan R, Kapoor N, Bharate SB, Hassan QP, Gandhi G (2022) Plant growth promoting potential of butyl isobutyl phthalate and Streptomyces sp. from Rumex dentatus on rice. Applied Microbiology and Biotechnology 106: 2603-2617. https://doi.org/10.1007/s00253-022-11862-w
Olowe OM, Nicola L, Asemoloye MD, Akanmu AO, Sobowale AA, Babalola OO (2022) Characterization and antagonistic potentials of selected rhizosphere Trichoderma species against some Fusarium species. Frontiers in Microbiology 13: 985874. https://doi.org/10.3389/fmicb.2022.985874
Pacios-Michelena S, Aguilar González C N, Alvarez-Perez OB, Rodriguez-Herrera R, Petkova M, Petrova S, Spasova-Apostolova V, Naydenov M (2022) Tobacco plant growth-promoting and antifungal activities of three endophytic yeast strains. Plants 11: 751. https://doi.org/10.3390/plants11060751
Rai GK, Mishra S, Chouhan R, Mushtaq M, Chowdhary AA, Rai PK, Kumar RR, Kumar P, Perez-Alfocea F, Colla G (2023) Plant salinity stress, sensing, and its mitigation through WRKY. Frontiers in Plant Science 14: 1238507. https://doi.org/10.3389/fpls.2023.1238507
Ranal MA, Santana DG (2006) How and why to measure the germination process? Brazilian Journal of Botany 29: 1-11. https://doi.org/10.1590/S0100-84042006000100002
Raza M, Ghazanfar MU, Hussain M, Iqbal Z, Iftikhar Y (2013) Pathogenic and non-pathogenic fungi isolated from soil and roots of tobacco. Pakistan Journal of Phytopathology 25: 95-100.
Ripa F, Nikkon F, Zaman S, Khondkar P (2009) Optimal conditions for antimicrobial metabolites production from a new Streptomyces sp. RUPA-08PR isolated from bangladeshi soil. Mycobiology 37(3): 211-214. https://doi.org/10.4489/MYCO.2009.37.3.211
Sati D, Pande V, Pandey SC, Samant M (2023) Recent advances in PGPR and molecular mechanisms involved in drought stress resistance. Journal of Soil Science and Plant Nutrition 23: 106-124. https://doi.org/10.1007/s42729-021-00724-5
Singh C, Parmar RS, Kumar A, Jadon P (2016) Characterization of actinomycetes against phytopathogenic fungi of Glycine max (L.). Asian Journal of Pharmaceutical and Clinical Research 9(1): 216-219.
Sosa-Sánchez O, Romero-Bastidas M, Silva-Pupo JJ, Gaibor-Fernández R, Rodríguez-Rodríguez S, Ceiro-Catasú W (2022) Caracterización cultural-morfométrica como herramienta para la identificación de Fusarium spp., asociado a Nicotiana tabacum L. Agronomía Costarricense 46(1): 111-128. https://doi.org/10.15517/rac.v46i1.49872
Statsoft (2011) STATISTICA User´s Guide. Version 8. SAS Inst., Cary, NC. USA. 1098p.
Terra L, Dyson P, Ratcliffe N, Castro HC, Vicente ACP (2021) Biotechnological potential of Streptomyces siderophores as new antibiotics. Current Medicinal Chemistry 28(7): 1407-1421. https://doi.org/10.2174/0929867327666200510235512
Toledo V (2014) Compatibilidad del control biológico de Trichoderma frente a fungicidas utilizados en el cultivo del tabaco. CUBA TABACO. 15(1): 3-9.
Ungar IA (1995) Seed germination and seed-bank ecology in halophytes. In: Kigel J, Galili G (eds) Seed development and germination. Marcel Dekker, Inc. New York. pp. 599-628.
Veobides-Amador H, Guridi-Izquierdo F, Vázquez-Padrón V (2018) Las sustancias húmicas como bioestimulantes de plantas bajo condiciones de estrés ambiental. Cultivos Tropicales 39(4): 102-109
Vurukonda SSKP, Giovanardi D, Stefani E (2018) Plant growth promoting and biocontrol activity of Streptomyces spp. as endophytes. International Journal of Molecular Sciences. 19(4): 952. https://doi.org/10.3390/ijms19040952
Wei C, Liang J, Wang R, Chi L, Wang W, Tan J, Shi H, Song X, Cui Z, Xie Q, Cheng D, Wang X (2024) Response of bacterial community metabolites to bacterial wilt caused by Ralstonia solanacearum: a multi-omics analysis. Frontiers in Plant Science 14: 1339478. https://doi:10.3389/fpls.2023.1339478
Wuana RA, Okieimen FE (2011) Heavy metals in contaminated soils: A review of sources, chemistry, risks and best available strategies for remediation. International Scholarly Research Notices 402647: 20. https://doi.org/10.5402/2011/402647
Yadav AN, Verma P, Kumar S, Kumar V, Kumar M, Sugitha TC, Singh BP, Saxena AK, Dhaliwal HS (2018) Actinobacteria from rhizosphere: molecular diversity, distributions, and potential biotechnological applications. In: Singh BP, Kumar-Gupta V, Kumar-Passari A (eds) New and future developments in microbial biotechnology and bioengineering. Elsevier. pp. 13-41. https://doi.org/10.1016/B978-0-444-63994-3.00002-3
Zhang H, Bai X, Han H, Han L (2023) Stress‑resistance and growth‑promoting characteristics and effects on vegetable seed germination of Streptomyces sp. strains isolated from wetland plant rhizospheres. Current Microbiology 80: 190. https://doi.org/10.1007/s00284-023-03297-x
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