Effects of Zinc Oxide nanoparticles and Zeolite on cage-free hens’ egg physical quality
DOI:
https://doi.org/10.19136/era.a13nVI.5181Keywords:
Cage free system, eggshell quality, mineral additivesAbstract
This study evaluates the effect of zinc oxide nanoparticles and zeolite added to the diet of cage-free hens on productive parameters and egg physical quality. A total of 400 hens, aged 44 to 60 weeks, were randomly assigned to four treatments: T1: 90 mg of zinc oxide nanoparticles per kg of feed (ZnO-NPs); T2: 90 mg of ZnO-NPs and 4% zeolite per kg of feed; T3: 4% zeolite per kg of feed; T4: Fed only the basal diet. Productivity variables (egg weight, number of eggs, percentage of egg production, egg mass) and physical quality variables (shape index, shell thickness and weight, albumen weight and height, yolk weight and diameter, and Haugh units) were measured. The data were analyzed using a one-way ANOVA, and principal component analysis (PCA) was used to identify variables associated with productivity and physical quality. Among the productivity variables, only feed intake showed significant differences, being higher in group T3 (p < 0.05). Regarding physical quality parameters, shell thickness was greater in T1 (p < 0.05) compared to the other experimental groups; for the remaining variables, no differences were observed between treatments (p > 0.05). These findings suggest that zinc oxide nanoparticles may influence shell thickness.
Downloads
References
Abedini M, Shariatmadari F, Karimi Torshizi MA, Ahmadi H (2018) Effects of zinc oxide nanoparticles on the egg quality, immune response, zinc retention, and blood parameters of laying hens in the late phase of production. Journal of Animal Physiology and Animal Nutrition 102(3): 736-745. https://doi.org/10.1111/jpn.12871
Anderson K, Tharrington JB, Curtis PA, Jones, FT (2004) Shell characteristics of eggs from historic strains of single comb white Leghorn chickens and the relationship of egg shape to shell strength. International Journal of Poultry Science 3: 1. https://doi.org/10.3923/ijps.2004.17.19
Barzegar S, Wu SB, Noble J, Choct M, Swick RA (201B9) Energy efficiency and net energy prediction of feed in laying hens. Poultry Science 98: 5746-5758. https://doi.org/10.3382/ps/pez362
Berto DA, Garcia EA, Pelícia K, Vercese F, Molino AB, da-Silveira AF, Vieira-Filho JA, Murakami ESF (2013) Effects of dietary clinoptilolite and calcium levels on the performance and egg quality of commercial layers. Revista Brasileira de Ciencia Avicola/Brazilian Journal of Poultry Science 15: 263-268. https://doi.org/10.1590/S1516-635X2013000300014
Buchwald Z, Sandomierski M, Voelkel A (2020) Calcium-Rich 13X Zeolite as a filler with remineralizing potential for dental composites. ACS Biomaterial Science and Engineering 6: 3843-3854. https://doi.org/10.1021/acsbiomaterials.0c00450
Cuca-García M, Ávila-González E, Pró-Martínez A (2008) Alimentación de las aves. Universidad Autónoma Chapingo. Mexico. 276p.
Demir E, Eseceli H, Özcan A (2017) Effect of activated Clinoptilolite in Aflatoxin b1 contaminated laying hen diets on aflatoxin b1 residues and quality of eggs. Zbornik Matice Srpske za Prirodne Nauke 133: 13-22. https://doi.org/10.2298/zmspn1733013d
Domel JR, House GM, Sobotik EB, Archer GS (2024) Evaluation of egg quality and performance in late-lay hens fed different combinations of Copper, Manganese, and Zinc complexed with sulfate or amino acid ion. Poultry 3: 36-46. https://doi.org/10.3390/poultry3010004
Elsherbeni AI, Youssef IM, Hamouda RE, Kamal M, El-Gendi GM, El-Garhi OH, Alfassam HE, Rudayni HA, Allam AA, Moustafa M, Mohammed OA, Mohammed K, Reda E (2024) Performance and economic efficiency of laying hens in response to adding zeolite to feed and litter. Poultry Science 103: 103799. https://doi.org/10.1016/j.psj.2024.103799
Emam KRS, Toraih HM, Hassan AM, El-Far AA, Morsy AS and Ahmed NA (2019) Effect of zeolite dietary supplementation on physiological responses and production of laying hens drinking saline well water in South Sinai. World Veterinary Journal 9(2): 109-122. https://doi.org/10.36380/scil.2019.wvj15
Hanusová E, Hrnčár C, Oravcová M, Chrastinová-Rajský M, Chrenková M, Hanus A (2021) The effect of natural zeolite dietary supplementation on the egg quality of Japanese Quails (Coturnix japonica) during the laying period. Journal of Microbiology, Biotechnology and Food Sciences 10: 1-5. https://doi.org/doi:10.15414/jmbfs.4631
Hassan S, Hassan F, Rehman MS (2020) Nano-Particles of trace minerals in poultry nutrition: potential applications and future prospects. 195: 591-612. https://doi.org/10.1007/s12011-019-01862-9
Hassan S, Sharif M, Mirza MA, Rehman MSU (2023) Effect of dietary supplementation of zinc nanoparticles prepared by different green methods on egg production, egg quality, bone mineralization, and antioxidant capacity in caged layers. Biological Trace Element Research 201(12): 5794-5804. https://doi.org/10.1007/s12011-023-03640-0
Ibrahim I, Natsir MH, Sjofjan O, Djunaidi IH, Susilo A, Rifa’i M, Hafsah H (2025) Optimization growth performance and intestinal characteristics of broiler through the use of zeolite and bioherbal-based mycotoxin binders as feed additives. Journal of Advance Veterinary Animal Research 12: 149-156. https://doi.org/10.5455/javar.2025.l882
INEGI (2025) Información Nacional Por Entidad Federativa y Municipios. Instituto Nacional de Estadística, Geografía e Informática. https://gaia.inegi.org.mx/mdm6/?v=bGF0OjI1LjU1Mjk1LGxvbjotMTAzLjM3NTM5LHo6MTUsbDpjMTExc2VydmljaW9zfHQxMTFzZXJ2aWNpb3M=. Data consulted: July 22, 2025.
Kumar A, Hosseindoust A, Kim M, Kim K, Choi Y, Lee S, Lee S, Lee J, Cho H, Kang WS, Chae B (2021) Nano-Sized Zinc in broiler chickens:Effects on growth performance, zinc concentration in organs, and intestinal morphology. Journal of Poultry Science 58: 21-29. https://doi.org/doi:10.2141/jpsa.0190115
Kumar S, Sahu SP, Kumari S, Sinha RRK, Kumar P, Kumar Sa, Kumar K (2024) Effects of liquid whey supplementation with water on egg production and egg quality in layer chickens. Indian Journal of Animal Research 58: 1053-1057. https://doi.org/10.18805/IJAR.B-5227
Lai PW, Liang JB, Hsia LC, Loh TC, Ho YW (2010) Effects of varying dietary zinc levels and environmental temperatures on the growth performance, feathering score and feather mineral concentrations of broiler chicks. Asian-Australas Journals Animals Science 23: 937-945. https://doi.org/10.5713/ajas.2010.90495
Li L, Miao L, Zhu M, Wang L, Zou X (2019) Dietary addition of zinc-methionine influenced eggshell quality by affecting calcium deposition in eggshell formation of laying hens. British Journal of Nutrition 122: 961-973. https://doi.org/10.1017/S000711451900206X
Lilburn MS, McIntyre DR (2024) An historical overview of zinc in poultry nutrition. Poultry Science 103: 104294. https://doi.org/10.1016/j.psj.2024.104294
Lonkar VD, Savaliya FP, Mishra RK, Patel AB, Bhagora NJ (2023) Effect of different nutrient density prelay diets on production performance of White Leghorn Layers. Indian Journal Animal Research 1: 1-5. https://doi.org/10.18805/ijar.b-4978
Medras ZJ, Al-khalaifah H, Khalid A, Mohamed, Hussain MA (2026) Dose-dependent effect of dietary zeolite on laying hens'performance. Frontiers in Animal Science 7: 1820752. https://doi.org/ 10.3389/fanim.2026.1820752
Mondal M, Biswas B, Garai S, Sarkar S, Banerjee H, Brahmachari K, Bandyopadhyay P K, Maitra S, Brestic M, Skalicky M, Ondrisik P, Hossain A (2021) Zeolites enhance soil health, crop productivity and environmental safety. Agronomy 11(3): 448. https://doi.org/10.3390/agronomy11030448
Monika M, Rokadae JJ, Narayan R, Verma MR, Panda S, Saharia J (2021) Genetic evaluation of egg production and egg quality attributes in japanese quails through partial periods. Indian Journal Animal Research 55: 1393-1400. https://doi.org/10.18805/IJAR.B-4205
Moss AF, Parkinson G, Crowley TM, Pesti GM (2021) Alternatives to formulate laying hen diets beyond the traditional least-cost model. Journal of Applied Poultry Research 30: 100137. https://doi.org/10.1016/j.japr.2020.100137
NRC (1994) Nutrient requirements of poultry: Ninth Revised Edition. National Research Council Nutrient. Washington, DC. 176p. https://www.nationalacademies.org/publications/2114. Date consulted: 14 december, 2025.
Naz S, Idris M, Khalique MA, Zia-Ur-Rahman, Alhidary IA, Abdelrahman MM, Khan RU, Chand N, Farooq U, Ahmad S (2016) The activity and use of zinc in poultry diets. Worlds Poultry Science Journal 72: 159-167. https://doi.org/10.1017/S0043933915002755
Rodríguez B, Valera M, Castro M (2024) Effect of micronized zeolite as an additive on production and egg quality in laying hens. Cuban Journal of Agricultural Science 58: 1-5. https://cu-id.com/1996/v58e13
Rokade J, Champati A, Sonale N, Wadajkar P, Madheshwaran M, Bhaisare D, Tiwari AK (2024) The cage-free egg sector: Perspectives of Indian poultry producers. Frontiers in Veterinary Science 11: 1442580. https://doi.org/10.3389/fvets.2024.1442580
Roland DA, Rabon HW, Rao KS, Smith RC, Miller JW, Barnes DG, Laurent SM (1993) Evidence for absorption of silicon and aluminum by hens fed sodium zeolite a. Poultry Science 72: 447-455. https://doi.org/10.3382/ps.0720447
Romo S, López D, Ledesma N, Gutiérrez C, Quintana A, Rangel L (2022) Comparison in the quality of eggs obtained in an outdoor pen production system and those produced in a cage system. Revista mexicana de ciencias pecuarias 13(1): 32-42. https://doi.org/10.22319/rmcp.v13i1.5300
Susilo A, Hanif M, Putra A, Ariyadi B, Agus A (2025) Performance of laying hens in different housing systems under tropical conditions. Advances in Animal and Veterinary Sciences 13(8): 1732-1740. https://dx.doi.org/10.17582/journal.aavs/2025/13.8.1732.1740
Taghiyev A, Huseynova N, Bayramova R (2025) The studing of additional technological feeding of white english quails with mineral substances and vitamins. Natural Science 7: 24-27. https://doi.org/10.36719/2707-1146/55/24-27
Ujilestari T, Adli DN, Alifian MD, Irawan A, Nurfitriani RA, Hudaya MF, Azmi AF, Sugiharto S, Sholikin MM (2025) Evaluating zeolite stability as a mycotoxin binder in broiler chickens’ growth performance: a meta-analysis. Iraqi Journal of Veterinary Sciences 39: 511-519. https://doi.org/10.33899/ijvs.2025.157757.4148
Vieira RB, Ludke MDCMM, Ludke JV, Lopes CC, Oliveira EF, Santos ACA, Coldebella A, Rabello CBV (2023) Effect of dietary zeolite supplementation on production, egg quality, ammonia volatilization, organ morphometry and blood parameters in brown laying hens. Brazilian Journal of Poultry Science 25. https://doi.org/10.1590/1806-9061-2023-1797
Zhang YN, Zhang HJ, Wang J, Yue HY, Qi XL, Wu SG, Qi GH (2017) Effect of dietary supplementation of organic or inorganic zinc on carbonic anhydrase activity in eggshell formation and quality of aged laying hens. Poultry Science 96: 2176-2183. https://doi.org/10.3382/ps/pew490
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Ecosistemas y Recursos Agropecuarios

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Aviso de copyright
Los autores que se envían a esta revista aceptan los siguientes términos:
una. Los autores conservan los derechos de autor y garantizan a la revista el derecho a ser la primera publicación del trabajo con una licencia de atribución de Creative Commons que permite a otros compartir el trabajo con un reconocimiento de la autoría del trabajo y la publicación inicial en esta revista.
B. Los autores pueden establecer acuerdos complementarios separados para la distribución no exclusiva de la versión del trabajo publicado en la revista (por ejemplo, en un repositorio institucional o publicarlo en un libro), con un reconocimiento de su publicación inicial en esta revista.
C. Se permite y se anima a los autores a difundir su trabajo electrónicamente (por ejemplo, en repositorios institucionales o en su propio sitio web) antes y durante el proceso de envío, ya que puede conducir a intercambios productivos, así como a una cita más temprana y más extensa del trabajo publicado. (Consulte El efecto del acceso abierto).






