Lippia graveolens extract on biochemical defense responses in peach trees [Prunus persica (L.) Batsch]
DOI:
https://doi.org/10.19136/era.a13n3.5186Keywords:
Peach tree, Lippia graveolens, defense enzymes, phenols, flavonoidsAbstract
Plant extracts are capable of inducing defense responses in plants by triggering enzymes and secondary metabolites. Lippia graveolens contains phenolic compounds with antifungal properties; however, its in vivo effects in fruit crops is little known. This assay assessed the effect of L. graveolens (5 500 mg L-1) extract over plant defense enzymes (phenylalanine ammonia-lyase, chitinase, β-1,3-glucanase, superoxide dismutase, catalase and glutathione peroxidase), phenols and flavonoids content, as well as over the incidence and severity of Alternaria tenuissima in peach trees [Prunus persica (L.) Batsch]. Five treatments in completely randomized design were applied: TeAb (control), TeIn (inoculated), InFu (inoculated + fungicide), TeLg (extract) and InLg (inoculated + extract). Treatment InLg significantly reduced the disease severity (49% Vs TeIn) and showed lower incidence, indicating the potential protective effect of the extract. Treatment InLg was linked to stronger activity of PAL, chitinase and β-1,3-glucanase in critical stages of the experiment, as well as to changes in the antioxidant response, in particular in the activity of superoxide dismutase. By large, the results suggest that L. graveolens extract has the potential to help manage descending necrosis in peach trees, caused by A. tenuissima.
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References
Albarracin-Gómez LD, Hortua-Gamboa SD, Acero-Godoy J (2022) Efecto inhibitorio del aceite esencial de Lippia graveolens sobre Fusarium oxysporum en la familia Solanaceae. Una revisión. Revista Tecnología en Marcha 36 (1): 54-65. https://doi.org/10.18845/tm.v36i1.5877
Ali N, Shoaib A, Rafiq M, Malik B, Yousaf M (2024) Vanillic acid enhances mung bean resistance and growth against Macrophomina phaseolina as a sustainable antifungal approach. Journal of Crop Health 76(6): 1473-1480. https://doi.org/10.1007/s10343-024-01062-z
Apel K, Hirt H (2004) Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology 55(1): 373-399. https://doi.org/10.1146/annurev.arplant.55.031903.141701
Baysal Ö, Zeller W (2004) Extract of Hedera helix induces resistance on apple rootstock M26 similar to Acibenzolar-S-methyl against fire blight (Erwinia amylovora). Physiological and Molecular Plant Pathology 65(6): 305-315. https://doi.org/10.1016/j.pmpp.2005.03.003
Bhuvaneshwari V, Paul PK (2012) Transcriptional and translational regulation of defense enzymes induced by neem fruit extract in tomato. Archives of Phytopathology and Plant Protection 45(12): 1374-1385. https://doi.org/10.1080/03235408.2012.655931
Bolboli Z, Tavakolian B, Mostowfizadeh-Ghalamfarsa R, Jafari M, Cacciola SO (2022) Stilbocrea banihashemiana sp. nov. a new fungal pathogen causing stem cankers and twig dieback of fruit trees. Journal of Fungi 8(7): 694. https://doi.org/10.3390/jof8070694
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72(1-2): 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
Burketova L, Trda L, Ott PG, Valentova O (2015) Bio-based resistance inducers for sustainable plant protection against pathogens. Biotechnology Advances 33(6): 994-1004. https://doi.org/10.1016/j.biotechadv.2015.01.004
Cabral-Miramontes JP, Martínez-Rocha AL, Rosales-Castro M, López-Rodríguez A, Meneses-Morales I, Del Campo-Quinteros E, Herrera-Ocelotol KK, Gándara-Moreno G, Velázquez-Huizar SJ, Ibarra-Sánchez L, Ruiz-Baca E (2024) Antifungal activity of mexican oregano (Lippia graveolens Kunth) extracts from industrial waste residues on Fusarium spp. in Bean Seeds (Phaseolus vulgaris L.). Agriculture 14(11): 1975. https://doi.org/10.3390/agriculture14111975
Cansev A, Gulen H, Eris A (2011) The activities of catalase and ascorbate peroxidase in olive (Olea europaea L. cv. Gemlik) under low temperature stress. Horticulture, Environment, and Biotechnology 52(2): 113-120. https://doi.org/10.1007/s13580-011-0126-4
Chowdhary VA, Tank JG (2023) Biomolecules regulating defense mechanism in plants. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences 93(1): 17–25. https://doi.org/10.1007/s40011-022-01387-7
Dėnė L, Valiuškaitė A (2021) Sensitivity of Botrytis cinerea isolates complex to plant extracts. Molecules 26(15): 4595. https://doi.org/10.3390/molecules26154595
Deresa EM, Diriba TF (2023) Phytochemicals as alternative fungicides for controlling plant diseases: A comprehensive review of their efficacy, commercial representatives, advantages, challenges for adoption, and possible solutions. Heliyon 9(3): e13810. https://doi.org/10.1016/j.heliyon.2023.e13810
Ei E, Park HH, Kuk YI (2024) Effects of plant extracts on growth promotion, antioxidant enzymes, and secondary metabolites in rice (Oryza sativa) plants. Plants 13(19): 2727. https://doi.org/10.3390/plants13192727
Ertani A, Pizzeghello D, Francioso O, Tinti A, Nardi S (2016) Biological activity of vegetal extracts containing phenols on plant metabolism. Molecules 21(2): 205. https://doi.org/10.3390/molecules21020205
Flohé L, Günzler WA (1984) Assays of glutathione peroxidase. Methods Enzymol 105(1): 114–120. https://doi.org/10.1016/S0076-6879(84)05015-1
Gholamnezhad J (2019) Effect of plant extracts on activity of some defense enzymes of apple fruit in interaction with Botrytis cinerea. Journal of Integrative Agriculture 18(1): 115-123. https://doi.org/10.1016/S2095-3119(18)62104-5
Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry 48(12): 909-930. https://doi.org/10.1016/j.plaphy.2010.08.016
Goswami SK, Singh V, Chakdar H, Choudhary P (2018) Harmful effects of fungicides-Current status. International Journal of Agriculture, Environment and Biotechnology 11(779): 1011-1019.
Grover A (2012) Plant chitinases: genetic diversity and physiological roles. Critical Reviews in Plant Sciences 31(1): 57-73. https://doi.org/10.1080/07352689.2011.616043
Hasanuzzaman M, Bhuyan MHM, Zulfiqar F, Raza A, Mohsin S, Mahmud J, Fujita M, Fotopoulos V (2020) Reactive oxygen species and antioxidant defense in plants under abiotic stress: Revisiting the crucial role of a universal defense regulator. Antioxidants 9(8): 681. https://doi.org/10.3390/antiox9080681
Jibril SM, Jakada BH, Kutama AS, Umar HY (2016) Plant and pathogens: pathogen recognision, invasion and plant defense mechanism. International Journal of Current Microbiology and Applied Sciences 5(6): 247-257. https://doi.org/10.20546/ijcmas.2016.506.028
Kadoo MR, Badere RS (2017) Modulation of the activity of chitinases and β-1, 3 glucanase in seedlings of cucumber and chilli by the aqueous extract of Cleistanthus collinus. Physiological and Molecular Plant Pathology 98: 46-53. https://doi.org/10.1016/j.pmpp.2017.03.004
Kadoo MR, Badere RS (2023) Enhancement of phenylalanine ammonia-lyase activity in cucumber and chilli seedlings by aqueous extract of Cleistanthus collinus. Indian Journal of Natural Products and Resources 14(4): 651-655. https://doi.org/10.56042/ijnpr.v14i4.6320
Khadivi A, Mirheidari F, Saeidifar A, Moradi Y, Tunç Y, Mishra DS (2025) Morphological and pomological characterizations of peach [Prunus persica (L.) Batsch] Accessions. Food Science & Nutrition 13(7): e70501. https://doi.org/10.1002/fsn3.70501
Kumari Y, Vengadaramana A (2017) Stimulation of defense enzymes in tomato (Solanum lycopersicum L.) and chilli (Capsicum annuum L.) in response to exogenous application of different chemical elicitors. Universal Journal of Plant Science 5(1): 10-15. 10.13189/ujps.2017.050102
Luo CX, Schnabel G, Hu M, De Cal A (2022) Global distribution and management of peach diseases. Phytopathology Research 4(1): 30. https://doi.org/10.1186/s42483-022-00134-0
Madden LV, Hughes G, Van den Bosch F (2007). The study of plant disease epidemics. American Phytopathological Society. St. Paul Minnesota, USA. xiv + 421 p.
Mahawer SK, Arya S, Kabdal T, Kumar R, Prakash O, Chitara MK, Koli P (2022) Plant defense systems: mechanism of self-protection by plants against pathogens. In: Soni R, Suyal Dc and Goel R (eds) Plant protection: from chemicals to biologicals. Walter de Gruyter GmbH. Berlín, Alemania. pp. 115-140. https://doi.org/10.1515/9783110771558-006
Maslennikova D, Ivanov S, Petrova S, Burkhanova G, Maksimov I, Lastochkina O (2023) Components of the phenylpropanoid pathway in the protective effect of SNP on wheat under salinity. Plants 12(11): 2123. https://doi.org/10.3390/plants12112123
Miller GL (1959) Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Analytical Chemistry 31(3): 426-428. https://doi.org/10.1021/ac60147a030
Muñoz-Rocha BG, Hernández-Pérez A, Moo-Huchín VM, Hernández-Castillo FD, Ochoa-Fuentes YM, Chávez-González ML, Carrillo-Lomelí DA, Peña-Ramos FM, Jasso de Rodríguez D (2025) Can phenolic compounds of semi-desert plant extracts control phytopathogenic fungi isolated from peach trees? Industrial Crops and Products 236: 121882. https://doi.org/10.1016/j.indcrop.2025.121882
Muñoz-Rocha BG, Jasso De Rodríguez D, Ochoa-Fuentes YM, Hernández-Pérez A, Hernández-Castillo FD, Velázquez-Guerrero JJ, Juárez-Maldonado A (2024) Hongos asociados a la necrosis descendente del duraznero en Valparaíso, Zacatecas, México: Primer reporte. Ecosistemas y Recursos Agropecuarios 11(3). https://doi.org/10.19136/era.a11n3.4163
Orozco-Santos M (1995) Enfermedades presentes y potenciales de los cítricos en México. Universidad Autónoma Chapingo. Chapingo, Edo. de México. 150p.
Quintero-Rodríguez E, Montoya-Vargas MP, López-Luján LM, Guzmán-Cabrera SA, Aristizábal-Sepúlveda OL, Bedoya-Pérez JC (2024) Evaluación del efecto de extractos vegetales para el control de enfermedades poscosecha del aguacate HASS. Revista Politécnica 20(40): 173-185. https://doi.org/10.33571/rpolitec.v20n40a11
Ramaroson ML, Koutouan C, Helesbeux JJ, Le Clerc V, Hamama L, Geoffriau E, Briard M (2022) Role of phenylpropanoids and flavonoids in plant resistance to pests and diseases. Molecules 27(23): 8371.
Ramos SJ, Faquin V, Guilherme LRG, Castro EM, Ávila FW, Carvalho GS, Bastos CEA, Oliveira C (2010) Selenium biofortification and antioxidant activity in lettuce plants fed with selenate and selenite. Plant, Soil and Environment 56(12): 584-588. https://doi.org/10.17221/113/2010-PSE
Rao MJ, Zheng B (2025) The role of polyphenols in abiotic stress tolerance and their antioxidant properties to scavenge reactive oxygen species and free radicals. Antioxidants 14(1): 74. https://doi.org/10.3390/antiox14010074
Rodríguez B, Pacheco L, Bernal I, Piña M (2023) Mechanisms of action of flavonoids: antioxidant, antibacterial and antifungal properties. Ciencia, Ambiente y Clima 6(2): 33-66. https://doi.org/10.22206/cac.2023.v6i2.3021.
Rodríguez-Pedroso AT, Ramírez-Arrebato NÁ, Cárdenas-Travieso RM (2006) Efecto de la quitosana en la inducción de la actividad de enzimas relacionadas con la defensa y protección de plántulas de arroz (Oryza sativa L.) contra Pyricularia grisea Sacc. Revista Mexicana de Fitopatología 24: 1-7.
Schöneberg T, Kibler K, Sulyok M, Musa T, Buchel TD, Mascher F, Bertossa M, Voegele RT, Vogelgsang S (2018) Can plant phenolic compounds reduce Fusarium growth and mycotoxin production in cereals? Food Additives & Contaminants: Part A 35 2455–2470. https://doi.org/10.1080/19440049.2018.1538570
SIAP (2024) Panorama Agroalimentario 2024. https://panorama.siap.gob.mx/vista/productos.php. Fecha de consulta: 10 de julio de 2024.
Singleton VL, Orthofer R, Lamuela-Raventós RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteau reagent. Methods in Enzymology 299: 152-178. https://doi.org/10.1016/S0076-6879(99)99017-1
Steiner AA (1961) A universal method for preparing nutrient solutions of a certain. Plant Soil 15(2): 134-154.
Sultana B, Anwar F, Ashraf M (2009) Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules 14(6): 2167-2180. https://doi.org/10.3390/molecules14062167
Sykłowska-Baranek K, Grech-Baran M, Naliwajski MR, Bonfill M, Pietrosiuk A (2015) Paclitaxel production and PAL activity in hairy root cultures of Taxus x media var. Hicksii carrying a taxadiene synthase transgene elicited with nitric oxide and methyl jasmonate. Acta Physiologiae Plantarum 37(10): 218. https://doi.org/10.1007/s11738-015-1949-x
Townsend GR, Heuberger JW (1943) Methods for estimating losses caused by diseases in fungicide experiments. Plant Disease Reporter 27: 340–343.
Tucuch-Pérez MA, Bojórquez-Vega JJ, Arredondo-Valdés R, Hernández-Castillo FD, Anguiano-Cabello JC (2021) Actividad biológica de extractos vegetales del semidesierto mexicano para manejo de Fusarium oxysporum de tomate. Ecosistemas y Recursos Agropecuarios 8(2). https://doi.org/10.19136/era.a8n2.2745
Valle-De la Paz M, Guillén-Sánchez D, Perales-Rosas D, López-Martínez V, Juárez-López P, Martínez-Fernández E, Hernández-Arenas M, Ariza-Flores R, Gijón-Hernández AR (2019) Distribución, incidencia y severidad de la muerte descendente (Lasiodiplodia spp.) en lima persa en Morelos, México. Revista Mexicana de Fitopatología 37(3): 464-478. https://doi.org/10.18781/r.mex.fit.1904-7
Vázquez-Cuecuecha OG, García-Gallegos E, Chávez-Gómez JA (2023) Caracterización física y química de los frutos de tres variedades de Prunus persica L. Batsch en Tlaxcala. Revista Mexicana de Ciencias Agrícolas 14(5): 85-94. https://doi.org/10.29312/remexca.v14i5.3197
Vyas P, Mukhopadhyay K (2018) Elicitation of phenylpropanoids and expression analysis of pal gene in suspension cell culture of Ocimum tenuiflorum L. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences 88: 1207-1217.
Woudenberg JHC, Groenewald JZ, Binder M, Crous PW (2013) Alternaria redefined. Studies in Mycology 75: 171-212. https://doi.org/10.3114/sim0015
Xue T, Hartikainen H, Piironen V (2001) Antioxidative and growth-promoting effect of selenium on senescing lettuce. Plant and Soil 237(1): 55-61. https://doi.org/10.1023/A:1013369804867
Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavening effects on superoxide radicals Food Chemistry 64(4): 555-559.
Van Loon LC, Rep M, Pieterse CMJ (2006) Significance of inducible defense-related proteins in infected plants. Annual Review of Phytopathology 44: 135–162. https://doi.org/10.1146/annurev.phyto.44.070505.143425
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