Evaluation of CaCO₃NPs obtained from maguey and effect on Raphanus sativus L. germination
DOI:
https://doi.org/10.19136/era.a13n3.5208Keywords:
Green synthesis, bio-waste, biostimulation, circular economyAbstract
Nanotechnology is a promising tool for addressing the challenges of agricultural productivity, especially in the development of nanofertilizers to improve nutrient uptake efficiency. This study evaluated the effect of calcium carbonate nanoparticles (CaCO3NPs) obtained from agave on the germination and vigor of Raphanus sativus L. seeds. Suspensions were applied at concentrations of 100, 200, and 300 mg L-1, in addition to a control with distilled water. The results indicated that the nanoparticles promoted a significant increase in the longitudinal growth of the seedlings (radicular length, hypocotyl length, and total length) at concentrations of 200 and 300 mg L-1. The hypocotyl diameter was greater at 200 mg L-1 but decreased at the highest concentration (300 mg L-1). In terms of biomass, the nanoparticle treatments showed a significant decrease compared to the control. The Dickson Quality Index (DQI), which assesses the overall quality of the seedlings, was significantly lower at concentrations of 100 and 300 mg L-1, while at 200 mg L-1 it remained similar to the control. These results suggest that CaCO3NPs positively affect morphological growth, but their impact on biomass and quality varies with the applied concentration.
Downloads
References
Abadía MB, Castillo LA, Alonso YN, Monterubbianesi MG, Maciel G, Bartosik RE (2024) Germination and vigor of maize seeds: Pilot-scale comparison of low-oxygen and traditional storage methods. Agriculture 14: 1268. https://doi.org/10.3390/agriculture14081268
Ahumada-Rudolph R, Nova M, Novoa V, Vergara-Figueroa J, Méndez P, Cabrera-Pardo J, González S, Cespedes-Acuña CL (2025) Advancing agricultural sustainability: Nanoparticles and zeolites for pathogenic fungal control and seed germination. Food Bioscience 68: 106409. https://doi.org/10.1016/j.fbio.2025.106409
Ali Q, Ali M, Jing H, Hussain A, Manghwar H, Ali M, Raza W, Mundra S (2024) Power of plant microbiome: A sustainable approach for agricultural resilience. Plant Stress 14: 100681. https://doi.org/10.1016/j.stress.2024.100681
Bhardwaj S, Verma T, Kour J, Singh AD, Bhardwaj R, Sharma NR, Ansari S, Raza A, Prasad PVV, Thakur U, Kapoor D (2024) Silicon and nitric oxide modulate growth attributes, antioxidant defense system and osmolytes accumulation in radish (Raphanus sativus L.) under arsenic toxicity. Plant Stress 12: 100473. https://doi.org/10.1016/j.stress.2024.100473
Bhatia A, Khatri A, Yadav M, Kumari A, Mona S, Rachna B (2025) Potential of iron oxide nanoparticles in enhancing growth and development of plants: A review. Physiological and Molecular Plant Pathology 139: 102746. https://doi.org/10.1016/j.pmpp.2025.102746
Di Benedetto A, Tognetti J (2016) Técnicas de análisis de crecimiento de plantas: Su aplicación a cultivos intensivos. Revista de Investigaciones Agropecuarias 42(3): 258-282.
Dickson A, Leaf AL, Hosner JF (1960) Quality appraisal of white spruce and white pine seedling stock in nurseries. The Forestry Chronicle 36: 10-13. https://doi.org/10.5558/tfc36010-1
Ferreira ES, Binotti FFS, Binotti EDC, Costa E, Vendruscolo EP, Lima SF, Coli Neto FA (2024) Potentiating Chlorella vulgaris bioinput as a growth biostimulant in the production of basil seedlings with the addition of vitamin B3. Algal Research 83: 103706. https://doi.org/10.1016/j.algal.2024.103706
Ende S, Henjes J, Spiller M, Elshobary M, Hanelt D, Abomohra A (2024) Recent advances in recirculating aquaculture systems and role of microalgae to close system loop. Bioresource Technology 407: 131107. https://doi.org/10.1016/j.biortech.2024.131107
Esquivias-Fierro A, González-López JR, Chávez-Guerrero L (2021) Surface‑assisted crystallization of highly pure CaCO3 films using bagasse ash as a raw material. Waste and Biomass Valorization 12(3): 1591-1597. https://doi.org/10.1007/s12649-020-01121-2
Gao Y, Chen S, Li Y, Shi Y (2023) Effect of nano-calcium carbonate on morphology, antioxidant enzyme activity and photosynthetic parameters of wheat (Triticum aestivum L.) seedlings. Chemical and Biological Technologies in Agriculture 10: 31. https://doi.org/10.1186/s40538-023-00404-9
Garza-Alonso CA, Cadenas-Pliego G, Juárez-Maldonado A,
González-Fuentes JA, Tortella G, Benavides-Mendoza A (2023a) Fe2O3 nanoparticles can replace Fe-EDTA fertilizer and boost the productivity and quality of Raphanus sativus in a soilless system. Scientia Horticulturae 321: 112261. https://doi.org/10.1016/j.scienta.2023.112261
Garza-Alonso CA, Juarez-Maldonado A, González-Morales S, Cabrera-De la Fuente M, Cadenas-Pliego G, Morales-Díaz AB, Trejo-Tellez LI, Tortella G, Benavides-Mendoza A (2023b) ZnO nanoparticles as potential fertilizer and biostimulant for lettuce. Heliyon 9. e12787 https://doi.org/10.1016/j.heliyon.2022.e12787
INEGI (2019) Marco Geoestadístico, septiembre 2019. Instituto Nacional de Estadística y Geografía, Aguascalientes, México.
Jan B, Bhat MA, Bhat TA, Nazir A, Dar KA, Kamran M, Soufan W, Abdelhamid MT, El-Sabagh A (2023) Measuring the influence of seedling age and nutrient sources on the performance of sweet corn (Zea mays L.) under temperate climatic conditions. ACS Omega 8 (21): 18981-18989. https://doi.org/10.1021/acsomega.3c01518
Kabilan M, Sakthivel N, Rajkishore SK, Ramah K, Rajeswari R, Surendrakumar A, Srimathi K, Vasumathi V (2025) Advancements in the realm of calcium nanoparticles: An in-depth exploration of synthesis, characterization and multifaceted applications in agriculture. Plant Science Today 12(Sp1): 1-13. https://doi.org/10.14719/pst.7518
Kasem MM, El-Baset MMA, Helaly AA, El-Boraie EA, Alqahtani MD, Alhashimi A, Abu-Elsaoud AM, Elkelish A, Mancy AG, Alhumaid A, El-Banna MF (2023) Pre and postharvest characteristics of Dahlia pinnata var. pinnata, cav. As affected by SiO2 and CaCO3 nanoparticles under two different planting dates. Heliyon 9: e17292. https://doi.org/10.1016/j.heliyon.2023.e17292
Klekotka U, Rogacz D, Szymanek I, Malejko J, Rychter P, Kalska-Szostko B (2022) Ecotoxicological assessment of magnetite and magnetite/ Ag nanoparticles on terrestrial and aquatic biota from different trophic levels. Chemosphere 308: 136207. https://doi.org/10.1016/j.chemosphere.2022.136207
Koley R, Mandal M, Mondal A, Debnath P, Mondal A, Mondal NK (2025) Synthesis of calcium carbonate nanoparticles from mollusc shell waste and its efficacy towards plant growth and development. Sustainable Chemistry One World 6: 100056. https://doi.org/10.1016/j.scowo.2025.100056
Kondo YR, da Cruz SP, Chanway C, Kaschuk G (2024) Inoculation with Azospirillum brasilense or Bacillus spp. improves root growth and nutritional quality of araucaria (Araucaria angustifolia) seedlings. Forest Ecology and Management 568: 122092. https://doi.org/10.1016/j.foreco.2024.122092
Kumar A, Verma LM, Sharma S, Singh N (2023) Zinc oxide nanoparticles (ZnO NPs) stabilized by phytocellulose derived biopolymer and their bipartite interaction studies with agriculturally important microbes / Raphanus sativus L. seeds. Ceramics International 49(24PA): 39771-39787. https://doi.org/10.1016/j.ceramint.2023.07.179
Mahawar L, Živčák M, Barboricova M, Kovár M, Filaček A, Ferencova J, Vysoká DM, Brestič M (2024) Effect of copper oxide and zinc oxide nanoparticles on photosynthesis and physiology of Raphanus sativus L. under salinity stress. Plant Physiology and Biochemistry 206: 108281. https://doi.org/10.1016/j.plaphy.2023.108281
Manokari M, Dey A, Faisal M, Alatar AA, Singh RK, Shekhawat MS (2023) Silicon nanoparticles (SiNPs) positively affect morpho-structural differentiation in micropropagated plantlets of Santalum album L. Silicon 15: 6843-6850. https://doi.org/10.1007/s12633-023-02558-5
Mazepa E, Malburg BV, Mógor G, de Oliveira AC, Amatussi JO, Corrêa DO, Lemos JS, Ducatti DRB, Duarte MER, Mógor AF, Noseda MD (2021) Plant growth biostimulant activity of the green microalga Desmodesmus subspicatus. Algal Research 59: 102434. https://doi.org/10.1016/j.algal.2021.102434
Mazhar MW, Ishtiaq M, Maqbool M, Akram R (2022) Seed priming with Calcium oxide nanoparticles improves germination, biomass, antioxidant defence and yield traits of canola plants under drought stress. South African Journal of Botany 151: 889-899. https://doi.org/10.1016/j.sajb.2022.11.017
Minaoui F, Hakkoum Z, Chabili A, Douma M, Mouhri K, Loudiki M (2024) Biostimulant effect of green soil microalgae Chlorella vulgaris suspensions on germination and growth of wheat (Triticum aestivum var. Achtar) and soil fertility. Algal Research 82: 103655. https://doi.org/10.1016/j.algal.2024.103655
Motlhalamme T, Kaningini AG, Thema FT, Mohale KC, Maaza M (2025) Nanotechnology in agriculture: Exploring the influence of calcium carbonate nanoparticles on tomato leaf and fruit metabolomic profiles. South African Journal of Botany 180: 615-624. https://doi.org/10.1016/j.sajb.2025.03.028
Poddar K, Sarkar D, Sarkar A (2020) Nanoparticles on photosynthesis of plants. In: Patra JK, Fraceto LF, Das G, Campos EVR (eds) Effects and role of green nanoparticles: Synthesis and biomedical applications. Springer International Publishing. Cham. pp. 273-287. https://doi.org/10.1007/978-3-030-39246-8_13
Riaz S, Hussain I, Parveen A, Ashraf MA, Rasheed R, Zulfiqar S, Thind S, Rehman S (2022) Silicon and nano-silicon in plant nutrition and crop quality. In: Etesami H, Al-Saeedi AH, El-Ramady H, Fujita M, Pessarakli M, Anwar-Hossain MBTS (eds) Silicon and nano-silicon in environmental stress management and crop quality improvement. Academic Press. pp. 277-295. https://doi.org/10.1016/B978-0-323-91225-9.00021-2
Rodríguez-Jurado S, Guevara-González RG, Aguirre-Becerra H, Esquivel-Escalante K, Feregrino-Pérez AA (2025) Nanoparticles as potential eustressors in plants. Agronomy 15: 2186. https://doi.org/10.3390/agronomy15092186
Rutkowski M, Krzemińska-Fiedorowicz L, Khachatryan K, Khachatryan G, Kalisz A, Sękara A (2024) Impact of silver nanoparticles in alginate gels on seed germination, growth and stress biochemical parameters of cucumber seedlings. Plant Stress 12: 100491. https://doi.org/10.1016/j.stress.2024.100491
Sahu S, Gautam S, Singh A, Lohani P, Sharma C, Pathak P, Kumar A, Singh H (2023) Synthesis and characterization of chitosan-zinc-salicylic acid nanoparticles: A plant biostimulant. International Journal of Biological Macromolecules 253: 127602. https://doi.org/10.1016/j.ijbiomac.2023.127602
Salama DM, Osman SA, Mahmoud SH, El-Tanahy AMM, El-aziz MEA (2024) Improving the productivity and physiological characteristics of lettuce plants using spraying calcium as a nanofertilizer. Horticulturae 10: 1157. https://doi.org/10.3390/horticulturae10111157
Santás-Miguel V, Arias-Estévez M, Rodríguez-Seijo A, Arenas-Lago D (2023) Use of metal nanoparticles in agriculture. A review on the effects on plant germination. Environmental Pollution 334: 122222. https://doi.org/10.1016/j.envpol.2023.122222
SIAP (2026) Cierre de la producción agrícola 2023 y 2024. Secretaría de Agricultura y Desarrollo Rural. Acciones y Programas. https://nube.agricultura.gob.mx/cierre_agricola/. Fecha de consulta: 15 de febrero de 2026.
Sorour MA (2021) Enhancing Cycas revoluta Thumb. Growth by calcium carbonate nanoparticles under water deficit stress condition. Scientific Journal Flowers & Ornamental Plants 8(1): 39-54. https://doi.org/10.21608/sjfop.2021.155058
Taheri N, Pishvaee MS, Jahani H, Zakeri D (2024) Towards sustainable resource allocation in agriculture: A systematic review on cropping pattern optimization approaches. Applied Soft Computing Journal 167: 112360. https://doi.org/10.1016/j.asoc.2024.112360
Tamindžić G, Ignjatov M, Miljaković D, Červenski J, Milošević D, Nikolić Z, Vasiljević S (2023) Seed priming treatments to improve heat stress tolerance of garden pea (Pisum sativum L.). Agriculture 13: 439. https://doi.org/10.3390/agriculture13020439
Tolisano C, Del-Buono D (2023) Biobased: Biostimulants and biogenic nanoparticles enter the scene. Science of the Total Environment 885: 163912. https://doi.org/10.1016/j.scitotenv.2023.163912
Tyagi L, Kadono H, Rajagopalan UM (2025) Rapid size-dependent impact of Cu and CuO nanoparticles on lentil seeds and leaves using biospeckle optical coherence tomography. Nanomaterials 15: 1214. https://doi.org/10.3390/nano15161214
Zamora-Ledezma E, Aragundi GLL, Guamán Marquines WS, Macías Pro MA, García Díaz JV, Pacheco Gil HA, Londoño JMB, Londoño MAB, Zamora-Ledezma C (2025) Phytotoxic effects and agricultural potential of nanofertilizers: A study using zeolite, zinc oxide, and titanium dioxide under controlled conditions. Journal of Xenobiotics 15: 123. https://doi.org/10.3390/jox15040123
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Ecosistemas y Recursos Agropecuarios

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Aviso de copyright
Los autores que se envían a esta revista aceptan los siguientes términos:
una. Los autores conservan los derechos de autor y garantizan a la revista el derecho a ser la primera publicación del trabajo con una licencia de atribución de Creative Commons que permite a otros compartir el trabajo con un reconocimiento de la autoría del trabajo y la publicación inicial en esta revista.
B. Los autores pueden establecer acuerdos complementarios separados para la distribución no exclusiva de la versión del trabajo publicado en la revista (por ejemplo, en un repositorio institucional o publicarlo en un libro), con un reconocimiento de su publicación inicial en esta revista.
C. Se permite y se anima a los autores a difundir su trabajo electrónicamente (por ejemplo, en repositorios institucionales o en su propio sitio web) antes y durante el proceso de envío, ya que puede conducir a intercambios productivos, así como a una cita más temprana y más extensa del trabajo publicado. (Consulte El efecto del acceso abierto).







