Evaluation of the lead removal capacity of Cladosporium sp.
DOI:
https://doi.org/10.19136/era.a13n3.4717Keywords:
Filamentous fungus, toxic metals, biosorption, Cladosporium spAbstract
Lead (Pb) is a highly toxic metal that can contaminate aquatic and terrestrial ecosystems. The objective of this study was to isolate and identify a filamentous fungus capable of removing Pb. Removal experiments were conducted at Pb concentrations of 50, 100, 250, 350, and 500 mg L-1. The parameters evaluated were hyphal morphology, Pb removal efficiency, elemental composition and fungal biomass production. Fungi strain was identified as Cladosporium sp. Scanning electron microscopy (SEM) revealed morphological changes at 350 and 500 mg L-1, including shorter, more compact, and deformed hyphae with granular deposits on their surface. Biomass production was not significantly affected, although reduced growth was observed at the highest Pb levels. Energy dispersive spectroscopy (EDS) confirmed Pb accumulation on the fungal surface, reaching 11.3% of the elemental composition in the 500 mg L-1 treatment. Pb removal efficiency increased with concentration and reached a maximum value of 97.3%.
Downloads
References
ATSDR (2025) Substance Priority List. Agency for Toxic Substances and Disease Registry. https://www.atsdr.cdc.gov/programs/substance-priority-list.html. Data consulted: February 7, 2025.
Bensch K, Braun U, Groenewald JZ, Crous PW (2012) The genus Cladosporium. Studies in Mycology 72(1): 1-401. https://doi.org/10.3114/sim0003.
Dhankhar R, Hooda A (2011) Fungal biosorption - an alternative to meet the challenges of heavy metal pollution inaqueous solutions. Environmental Technology 32 (5): 467-491 https://doi.org/10.1080/09593330.2011.572922.
El-Gendy MMAA, Abdel-Moniem SM, Ammar NS, El-Bondkly AMA (2023) Bioremoval of heavy metals from aqueous solution using dead biomass of indigenous fungi derived from fertilizer industry effluents: isotherm models evaluation and batch optimization. BioMetals 36: 1307-1329. https://doi.org/10.1007/s10534-023-00520-x.
El-Morsy ESM, Abdel-Azeem AM, Rashad HM (2023) Biosorption of heavy metals by dead biomass of Mucor hiemalis Wehmer and Trichoderma viride Pers. in separate and consortium systems. Scientific Journal for Damietta Faculty of Science 13(2): 57-65.
El-Sherif IY, Tolani S, Ofosu K, Mohamed OA, Wanekaya AK (2013) Polymeric nanofibers for the removal of Cr(III) from tannery wastewater. Journal of Environmental Management 129: 410-413. https://doi.org/doi: 10.1016/j.jenvman.2013.08.004
Fomina M, Gadd GM (2014) Biosorption: current perspectives on concept, definition and application. Bioresource Technology 160: 3e14. https://doi.org/10.1016/j.biortech.2013.12.102
Fu F, Wang Q (2011) Removal of heavy metal ions from wastewaters: A review. Journal of Environmental Management 92: 407-418. https://doi.org/10.1016/j.jenvman.2010.11.011
Gadd GM (2007) Geomycology: biogeochemical transformations of rocks, minerals, metals and radionuclides by fungi, bioweathering and bioremediation. Mycological Research 111: 3-49. https://doi.org/10.1016/j.mycres.2006.12.001
Gadd GM (2009) Biosorption: critical review of scientific rationale, environmental importance and significance for pollution treatment. Journal of Chemical Technology and Biotechnology 84: 13-28
Gadd GM (2010) Metals, minerals and microbes: geomicrobiology and bioremediation. Microbiology 156: 609-643p. https://doi.org/10.1099/mic.0.037143-0
Garcia-Rubio R, de Oliveira HC, Rivera J, Trevijano-Contador N (2020) The fungal cell wall: Candida, Cryptococcus, and Aspergillus species. Frontiers in Microbiology 10: 2993, https://doi.org/10.3389/fmicb.2019.02993
Lee J, Kim S, Lee JU, Shin KS, Hur HG (2022) Highly effective biosorption capacity of Cladosporium sp. strain F1 to lead phosphate minerals and lead iodide from perovskite solar cells. Environmental Technology & Innovation 28: 102950. https://doi.org/10.1016/j.eti.2022.102950
Kurniati E, Arfarita N, Imai T (2014) Potential use of Aspergillus flavus strain KRP1 in utilization of mercury contaminant. Procedia Environmental Sciences 20: 254-260. https://ddoi.org/10.1016/j.proenv.2014.03.032
Saeidi N, Parvini M, Niavarani Z (2015) High surface area and mesoporous graphene/ activated carbon composite for adsorption of Pb(II) from wastewater. Journal of Environmental Chemical Engineering 3: 2697–2706. https://doi.org/10.1016/j.jece.2015.09.023
Saha L, Tiwari J, Bauddh K, Ma Y (2021) Recent developments in microbe–plant-based bioremediation for tackling heavy metal-polluted soils. Frontiers in Microbiology 12: 731723. https://doi.org/10.3389/fmicb.2021.731723
Sparks DL (2003) Environmental Soil Chemistry. 2nd Edition. Academic Press, San Diego, California, USA. 352p.
Senol ZM, Gül ÜD, Gurbanov R, Simsek S (2021) Optimization the removal of lead ions by fungi: Explanation of the mycosorption mechanism. Journal of Environmental Chemical Engineering 9: 104760. https://doi.org/104760 10.1016/j.jece.2020.104760
Sivan A, Szanto M, Pavlov V (2006) Biofilm development of the polyethylene degrading bacterium Rhodococcus ruber. Applied Microbiology and Biotechnology 72 (2): 346–352. https://doi.org/10.1007/s00253-005-0259-4
Srivastava S, Thakur IS (2006) Isolation and process parameter optimization of Aspergillus sp. for removal of chromium from tannery effluent. Bioresource Technology 97: 1167-1173. https://doi.org/10.1016/j.biortech.2005.05.012
Taseidifar M, Makavipour F, Pashley RM, Rahman AFM (2017) Removal of heavy metal ions from water using ion flotation. Environmental Technology & Innovation 8: 182-190. https://doi.org/10.1016/j.eti.2017.07.002
Titilawo MA, Ojo DA, Babalola OO (2023) Evaluation of lead tolerance and biosorption characteristics of fungi isolated from dumpsite soils. Discover Environment 3: 25. https://doi.org/10.1007/s44274-023-00025-x
Velásquez L, Dussan J (2009) Biosorption and bioaccumulation of heavy metals on dead and living biomass of Bacillus sphaericus. Journal of Hazardous Materials 167(1-3): 713-716. https://doi.org/10.1016/j.jhazmat.2009.01.044
Wang J, Chen C (2009) Biosorbents for heavy metals removal and their future. Biotechnology Advances 27: 195-226. https://doi.org/10.1016/j.biotechadv.2008.11.002
Wang Y, Yi B, Sun X, Yu L, Wu L, Liu W, Wang D, Li Y, Jia R, Yu H, Li X (2019) Removal and tolerance mechanism of Pb by a filamentous fungus: A case study, Chemosphere 225: 200-208. https://doi.org/10.1016/j.chemosphere.2019.03.027
White TJ, Burns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In MA Innis, DH Gelfand, JJ Snisky, T JWhite (Eds.), PCR protocols: A guide to methods and applications (pp. 315–322). San Diego, USA: Academic Press. https://doi.org/10.1016/b978-0-12-372180-8.50042-1
WHO (2025) Lead poisoning. World Health Organization. https://www.who.int/news-room/fact-sheets/detail/lead-poisoning-and-health. Data consultation: January 30, 2025.
Zhang S, Zhang X, Chang C, Yuan Z, Wang T, Zhao Y, Yang X, Zhang Y, La G, Wu K, Zhang Z, Li X (2016) Improvement of tolerance to lead by filamentous fungus Pleurotus ostreatus HAU-2 and its oxidative responses. Chemosphere 150: 33-39. https://doi.org/10.1016/j.chemosphere.2016.02.003
Zhao WW, Zhu G, Daugulis AJ, Chen Q, Ma HY, Zheng P, Liang J, Ma XK (2020) Removal and biomineralization of Pb2+ in water by fungus Phanerochaete chrysosporium. Journal of Cleaner Production 260: 120980. https://doi.org/10.1016/j.jclepro.2020.120980
Zou Y, Wang X, Khan A, Wang P, Liu Y, Alsaedi A, Hayat T, Wang X (2016) Environmental remediation and application of nanoscale zerovalent iron and its composites for the removal of heavy metal ions: a review. Environmental Science & Technology 50: 7290-7304
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Ecosistemas y Recursos Agropecuarios

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Aviso de copyright
Los autores que se envían a esta revista aceptan los siguientes términos:
una. Los autores conservan los derechos de autor y garantizan a la revista el derecho a ser la primera publicación del trabajo con una licencia de atribución de Creative Commons que permite a otros compartir el trabajo con un reconocimiento de la autoría del trabajo y la publicación inicial en esta revista.
B. Los autores pueden establecer acuerdos complementarios separados para la distribución no exclusiva de la versión del trabajo publicado en la revista (por ejemplo, en un repositorio institucional o publicarlo en un libro), con un reconocimiento de su publicación inicial en esta revista.
C. Se permite y se anima a los autores a difundir su trabajo electrónicamente (por ejemplo, en repositorios institucionales o en su propio sitio web) antes y durante el proceso de envío, ya que puede conducir a intercambios productivos, así como a una cita más temprana y más extensa del trabajo publicado. (Consulte El efecto del acceso abierto).






