Copper nanoparticles impact: Enzymatic activity, capsaicinoids, bioactive compounds and copper concentration in habanero peppers

Authors

  • Alexis Gabriel Pivaral Chávez Universidad Autónoma Agraria Antonio Narro image/svg+xml
    • Project Administration
    • Writing – Original Draft Preparation
    • Methodology
    • Investigation
  • Eduardo Arón Flores Hernández Universidad Autónoma Agraria Antonio Narro image/svg+xml
    • Investigation
    • Resources
    • Supervision
  • Urbano Nava Camberos Juarez University of the State of Durango image/svg+xml
    • Visualization
  • Alma Patricia Galindo Guzmán Universidad Politécnica de la Región Laguna
    • Writing – Review & Editing
  • Antonio Castillo Martínez Universidad Autónoma Agraria Antonio Narro image/svg+xml
    • Data Curation
  • Alejandro Moreno Reséndez Universidad Autónoma Agraria Antonio Narro image/svg+xml
    • Formal Analysis

DOI:

https://doi.org/10.19136/era.a13n3.5190

Keywords:

Capsaicin, antioxidant capacity, catalase, dihydrocapsaicin, peroxidase

Abstract

The response of habanero pepper (Capsicum chinense Jacq.) to copper nanoparticles (Cu-NPs) application was analyzed by assessing enzymatic activity, bioactive compounds, capsaicinoids, and Cu concentration. The experiment was conducted using a completely randomized design with four treatments: a control and three Cu-NPs concentrations (2, 4, and 6 mg L-1), with five replicates per treatment. Foliar applications began 20 days after transplanting and were repeated at 15-day intervals, for a total of four applications. Fruit yield, bioactive compound content, enzymatic activity, and Cu concentration were determined in the harvested fruits. The highest concentration (6 mg L-1) significantly increased peroxidase activity (87%), antioxidant capacity (48%), total flavonoids (42%), and dihydrocapsaicin biosynthesis (22%). The intermediate concentration (4 mg L-1) increased catalase activity by 79%, whereas capsaicin and total phenol contents were not affected by the treatments. The intermediate and highest concentrations (4 and 6 mg L-1) increased Cu accumulation in the fruits by up to 20%, indicating a concentration-dependent response and potential for biofortification. Fruit yield did not differ significantly among treatments. Cu-NPs may enhance the antioxidant system, bioactive metabolites, and Cu concentration without affecting fruit production.

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References

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Published

2026-09-30

Issue

Section

SCIENTIFIC ARTICLE

How to Cite

Pivaral Chávez, A. G., Flores Hernández, E. A., Nava Camberos, U., Galindo Guzmán, A. P., Castillo Martínez, A., & Moreno Reséndez, A. (2026). Copper nanoparticles impact: Enzymatic activity, capsaicinoids, bioactive compounds and copper concentration in habanero peppers. Ecosistemas Y Recursos Agropecuarios, 13(3), e5190. https://doi.org/10.19136/era.a13n3.5190

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