Effect of abscisic acid and 24-epibrasinolide on the nutritional status of tomato under calcium deficiency
DOI:
https://doi.org/10.19136/era.a13n2.5053Keywords:
Growth regulators, abiotic stress, physiological disordersAbstract
The tomato (Solanum lycopersicum L.) is the second most cultivated vegetable worldwide, and its production faces challenges due to climate change. Calcium deficiency, a recurring abiotic stress, causes physiological alterations that compromise the crop's nutritional status and, consequently, its yield. To counteract this problem, the use of plant growth regulators has been proposed. The objective of this research was to evaluate the effect of abscisic acid (ABA) and 24-epibrassinolide (EBL) on the nutritional status of tomatoes under calcium deficiency (45 mg L-1). A completely randomized 4 x 4 factorial experiment was established. Four concentrations of ABA (0, 60, 100 and 140 mg L⁻¹) and four of EBL (0, 0.0024, 0.0048 and 0.0096 mg L⁻¹) were evaluated as factors. The foliar concentration of nitrogen, magnesium, sulfur, and boron increased significantly with ABA2EBL1, while for phosphorus and manganese, ABA2EBL0 showed the best results. In the fruit, treatments ABA1EBL0 and ABA3EBL0 resulted in the highest nutrient concentrations in the first evaluation. In the second evaluation, ABA3EBL0 produced the highest concentrations of phosphorus, magnesium, and boron, while ABA2EBL0 stood out for its manganese content. In the third evaluation, the positive effect of most treatments was less pronounced. The results demonstrate that the application of ABA and EBL increases the concentration of macro and micronutrients in tomato fruits during the intermediate production phase, specifically in plants with calcium deficiency.
Downloads
References
Ahmad P, Ahanger MA, Egamberdieva D, Alam P, Alyemeni MN, Ashraf M (2018) Modification of osmolytes and antioxidant enzymes by 24-epibrassinolide in chickpea seedlings under mercury (Hg) toxicity. Journal of Plant Growth Regulation 37(1): 309-322. https://doi.org/10.1007/s00344-017-9730-6
Ahmed M, Babayola M, Bake ID (2024) Role of horticultural crops in food and nutritional security: A review. Journal of Nutrition and Food Processing 7(8): 01-06. https://doi.org/10.31579/2637-8914/226
Alcántar GG, Sandoval VM (1999) Manual de análisis químico de tejido vegetal. Publicación especial No. 10. Sociedad Mexicana de la Ciencia del Suelo, A. C. Chapingo, Edo. de México. 156p.
Ali MM, Anwar R, Malik AU, Khan AS, Ahmad S, Hussain Z, Hasan MU, Nasir M, Chen F (2022) Plant growth and fruit quality response of strawberry is improved after exogenous application of 24-epibrassinolide. Journal of Plant Growth Regulation 41(4): 1786-1799. https://doi.org/10.1007/s00344-021-10422-2
Barickman TC, Kopsell DA, Sams CE (2019) Applications of abscisic acid and increasing concentrations of calcium affect the partitioning of mineral nutrients between tomato leaf and fruit tissue. Horticulturae 5(3): 49. https://doi.org/10.3390/horticulturae5030049
Brown PH, Shelp BJ (1997) Boron mobility in plants. Plant and Soil 193: 85-101. https://doi.org/10.1023/A:1004211925160
Bukhari SAH, Wang R, Wang W, Ahmed IM, Zheng W, Cao F (2016) Genotype-dependent effect of exogenous 24-epibrassinolide on chromium-induced changes in ultrastructure and physicochemical traits in tobacco seedlings. Environmental Science and Pollution Research 23(18): 18229-18238. https://doi.org/10.1007/s11356-016-7017-2
Bulgakov VP, Koren OG (2022) Basic protein modules combining abscisic acid and light signaling in Arabidopsis. Frontiers in Plant Science 12: 808960. https://doi.org/10.3389/fpls.2021.808960
Choi EY, Park HI, Ju JH, Yoon YH (2015) Boron availability alters its distribution in plant parts of tomato. Horticulture, Environment, and Biotechnology 56(2): 145-151. https://doi.org/10.1007/s13580-015-0044-y
De-Freitas ST, McElrone AJ, Shackel KA, Mitcham EJ (2014) Calcium partitioning and allocation and blossom-end rot development in tomato plants in response to whole-plant and fruit-specific abscisic acid treatments. Journal of Experimental Botany 65(1): 235-247. https://doi.org/10.1093/jxb/ert364
Díaz-Pérez JC, Hook JE (2017) Plastic-mulched bell pepper (Capsicum annuum L.) plant growth and fruit yield and quality as influenced by irrigation rate and calcium fertilization. HortScience 52: 774-781. https://doi.org/10.21273/HORTSCI11830-17
Dos-Santos LA, Batista BL, Lobato AKDS (2023) 24-Epibrasinolide delays chlorophyll degradation and stimulates the photosynthetic machinery in magnesium-stressed soybean plants. Journal of Plant Growth Regulation 42(1): 183-198. https://doi.org/10.1007/s00344-021-10539-4
FAO (2024) Agricultural Production Statistics 2010–2023. FAOSTAT Analytical Briefs, No. 96. https://openknowledge.fao.org/server/api/core/bitstreams/df90e6cf-4178-4361-97d4-5154a9213877/content. Fecha de consulta: 20 de enero de 2026.
FAOSTAT (2023) Cultivos y productos de ganadería 2023. https://www.fao.org/faostat/es/#data/QCL. Fecha de consulta: 15 de octubre de 2025.
Gao H, Wu X, Zorrilla C, Vega SE, Palta JP (2020) Fractionating of calcium in tuber and leaf tissues explains the calcium deficiency symptoms in potato plant overexpressing CAX1. Frontiers in Plant Science 10: 1793. https://doi.org/10.3389/fpls.2019.01793
Kabir MY, Díaz-Pérez JC 2025 Calcium route in the plant and blossom-end rot incidence. Horticulturae 11(7): 807. https://doi.org/10.3390/horticulturae11070807
Khatri P, Kumar P, Shakya KS, Kirlas MC, Tiwari KK (2024) Understanding the intertwined nature of rising multiple risks in modern agriculture and food system. Environment, Development and Sustainability 26(9): 24107-24150. https://doi.org/10.1007/s10668-023-03638-7
López-Ruiz BA, Zluhan-Martínez E, Sánchez MDLP, Álvarez-Buylla ER, Garay-Arroyo A (2020) Interplay between hormones and several abiotic stress conditions on Arabidopsis thaliana primary root development. Cells 9(12): 2576. https://doi.org/10.3390/cells9122576
Maia CF, Pereira YC, Da Silva BRS, Batista BL, Lobato AKDS (2023) Exogenously applied 24-epibrassinolide favours stomatal performance, ROS detoxification and nutritional balance, alleviating oxidative damage against the photosynthetic apparatus in tomato leaves under nickel stress. Journal of Plant Growth Regulation 42(4): 2196-2211. https://doi.org/10.1007/s00344-022-10693-3
Mishra A (2021) Physiological disorders of tomato-an overview. International Journal of Farm Sciences 11(4): 46-50. https://doi.org/10.5958/2250-0499.2021.00054.9
Nazir F, Hussain A, Fariduddin Q (2019) Interactive role of epibrassinolide and hydrogen peroxide in regulating stomatal physiology, root morphology, photosynthetic and growth traits in Solanum lycopersicum L. under nickel stress. Environmental and Experimental Botany 162: 479-495. https://doi.org/10.1016/j.envexpbot.2019.03.021
R Core Team (2023) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/. Fecha de consulta: 1 de octubre de 2025.
Riboldi LB, Gaziola SA, Azevedo RA, De-Freitas ST, De-Camargo e Castro PR (2019) 24-Epibrassinolide mechanisms regulating blossom-end rot development in tomato fruit. Journal of Plant Growth Regulation 38(3): 812-823. https://doi.org/10.1007/s00344-018-9892-x
Riboldi LB, Da-Cruz-Araújo SH, Murcia JAG, De-Freitas ST, De-Camargo-e-Castro PR (2018) Abscisic acid and 24-epibrassinolide regulate blossom-end rot (BER) development in tomato fruit under Ca2+ deficiency. Australian Journal of Crop Science 12(9): 1440-1446. https://doi.org/10.21475/ajcs.18.12.09.PNE1106
Sánchez-Aguilar AB, Sandoval-Villa M, Trejo-Téllez LI, Suárez-Espinosa J, Fernández-Pavía YL (2025) Blossom-end rot control in tomato (Solanum lycopersicum L.) fruit using plant growth regulators. Agrociencia 59(6): 1-17. https://doi.org/10.47163/agrociencia.v59i6.3331
Seleiman MF, Kheir AM (2018) Maize productivity, heavy metals uptake and their availability in contaminated clay and sandy alkaline soils as affected by inorganic and organic amendments. Chemosphere 204: 514-522. https://doi.org/10.1016/j.chemosphere.2018.04.073
Shah SH, Islam S, Alamri S, Parrey ZA, Mohammad F, Kalaji HM (2023). Plant growth regulators mediated changes in the growth, photosynthesis, nutrient acquisition and productivity of mustard. Agriculture 13(3): 570. https://doi.org/10.3390/agriculture13030570
Shahzad B, Tanveer M, Che Z, Rehman A, Cheema SA, Sharma A, Song H, Rehman S, Zhaorong D (2018) Role of 24-epibrassinolide (EBL) in mediating heavy metal and pesticide induced oxidative stress in plants: a review. Ecotoxicology and Environmental Safety 147: 935-944. https://doi.org/10.1016/j.ecoenv.2017.09.066
SIAP (2022) Cierre agrícola. Gobierno de México. Servicio de Información Agroalimentaria y Pesquera. Ciudad de México, México. https://www.gob.mx/cms/uploads/attachment/file/732608/Jitomate_Mayo.pdf/. Fecha de consulta: 1 de noviembre de 2025.
Soylemez S, Kaya C, Dikilitas SK (2017) Promotive effects of epibrassinolide on plant growth, fruit yield, antioxidant, and mineral nutrition of saline stressed tomato plants. Pakistan Journal of Botany 49(5): 1655-1661.
Steiner AA (1984) The Universal Nutrient Solution, Proceedings of IWOSC 1984 6th International Congress on Soilless Culture. Wageningen. The Netherlands. pp. 633-650.
Topcu Y, Nambeesan SU, Van Der Knaap E (2022) Blossom-end rot: a century-old problem in tomato (Solanum lycopersicum L.) and other vegetables. Molecular Horticulture 2(1): 1. https://doi.org/10.1186/s43897-021-00022-9
Zhang Y, Liang Y, Han J, Hu X, Li X, Zhao H, Bai L, Shi Y, Ahammed GJ (2023) Interactive effects of iron and photoperiods on tomato plant growth and fruit quality. Journal of Plant Growth Regulation 42(1): 376-389. https://doi.org/10.1007/s00344-021-10554-5
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Ecosistemas y Recursos Agropecuarios

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Aviso de copyright
Los autores que se envían a esta revista aceptan los siguientes términos:
una. Los autores conservan los derechos de autor y garantizan a la revista el derecho a ser la primera publicación del trabajo con una licencia de atribución de Creative Commons que permite a otros compartir el trabajo con un reconocimiento de la autoría del trabajo y la publicación inicial en esta revista.
B. Los autores pueden establecer acuerdos complementarios separados para la distribución no exclusiva de la versión del trabajo publicado en la revista (por ejemplo, en un repositorio institucional o publicarlo en un libro), con un reconocimiento de su publicación inicial en esta revista.
C. Se permite y se anima a los autores a difundir su trabajo electrónicamente (por ejemplo, en repositorios institucionales o en su propio sitio web) antes y durante el proceso de envío, ya que puede conducir a intercambios productivos, así como a una cita más temprana y más extensa del trabajo publicado. (Consulte El efecto del acceso abierto).