Centropomus undecimalis: Growth performance, hematological responses, and unitary feeding cost with graded soybean meal substitution
DOI:
https://doi.org/10.19136/era.a13n2.4981Keywords:
digestive physiology, erythrocyte morphometry, plant-based feed, sustainable feeding, economic viabilityAbstract
This study evaluated the effects of replacing fishmeal (FM) with soybean meal (SM) in diets for juvenile common snook (Centropomus undecimalis) over a 120-day feeding trial. The relevance of this work lies in its contribution to sustainable aquaculture nutrition by partially substituting FM with SM, thereby reducing production costs and dependence on marine-derived inputs without compromising growth performance. Three diets containing 45% crude protein were formulated, in which FM was replaced at 0%, 35%, and 55% with SM. Each diet was administered to three groups of 16 fish, with an initial mean weight of 25.4 g and length of 15.5 cm. Growth parameters, digestive enzyme activity, and hematological variables were assessed. No significant differences were observed among treatments for growth, survival, or hematological parameters. However, the diet containing 55% SM reduced acidic protease activity, while the 35% SM diet significantly decreased feeding costs compared with the 0% and 55% SM diets. In conclusion, replacing 35% of FM with SM may be a promising alternative for juvenile snook culture, as it reduced feeding costs without negatively affecting growth, survival, or hematological parameters under the conditions evaluated.
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Abasubong KP, Cheng HH, Li ZQ, Wang CC, Huang YY, Liu WB, Jiang GZ (2021) Effects of replacing fish meal with plant proteins at different dietary protein levels on growth and feed intake regulation of juvenile channel catfish Ictalurus punctatus. Aquaculture Research 52(10): 4911-4922. https://doi.org/10.1111/are.15369.
Abdo-de la Parra MI, Rodríguez-Ibarra LE, Ibarra-Castro L, Martínez-Brown JM, Álvarez-González CA, Peña E, Rodríguez-Montes de Oca G (2023) Evaluation of different levels of dietary protein and lipids on the growth, feed efficiency, and biometric and hematological indexes of juvenile white snooks Centropomus viridis. Ciencias Marinas 49: e3368. https://doi.org/10.7773/cm.y2023.3368
Alvarez-González CA, Gaxiola-Cortés G, Jiménez-Martínez LD, Sánchez-Zamora A, Arena-Ortiz L, Martínez-Bruguete T, Jesús-Ramírez F (2010) Avances en la fisiología digestiva del robalo blanco (Centropomus undecimalis) en Tabasco, México. Avances en Nutrición Acuícola. https://nutricionacuicola.uanl.mx/index.php/acu/article/view/106. Retrieved: octuber 3, 2025.
Alvarez-Lajonchére L, Hernández Molejón OG (2001) Producción de juveniles de peces estuarinos para un centro en América Latina y el Caribe: diseño, operación y tecnologías. The World Aquaculture Society, Baton Rouge, LA. 424p.
Alvarez-Lajonchère L, Tsuzuki MY (2008) A review of methods for Centropomus spp. (snooks) aquaculture and recommendations for the establishment of their culture in Latin America. Aquaculture Research 39(7): 684-700. https://doi.org/10.1111/j.1365-2109.2008.01921
Anson ML (1938) The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin. The Journal of general physiology 22(1): 79. https://doi.org/10.1085/jgp.22.1.79
Arenas M, Alvarez-González A, Barreto A, Sánchez A, Suárez-Bautista J, Escalante K, Gaxiola G (2022) Effect of dietary carbohydrates on growth performance, feed efficiency and glucose metabolism in common snook (Centropomus undecimalis) and yellowtail snapper (Ocyurus chrysurus) juveniles. Aquaculture 543: 736958. https://doi.org/10.1016/j.aquaculture.2021.736958
Arriaga-Hernández D, Hernández C, Martínez-Montaño E, Ibarra-Castro L, Lizárraga-Velázquez E, Leyva-López N, Chávez-Sánchez MC (2021) Fish meal replacement by soybean products in aquaculture feeds for white snook Centropomus viridis: Effect on growth, diet digestibility, and digestive capacity. Aquaculture 530: 735823. https://doi.org/10.1016/j.aquaculture.2020.735823
Atencio-García V, Genes LF, López M, Madariaga MD, Pardo CS (2007) Hematología y química sanguínea de juveniles de rubio (Salminus affinis Pisces: Characidae) del río Sinú. Acta Biológica Colombiana 125: 27–40.
Bakke-McKellep AM, Frøystad M, Lilleeng E, Dapra F, Refstie S, Krogdahl A, Landsverk T (2007). Response to soy: T‐cell‐like reactivity in the intestine of Atlantic salmon, Salmo salar L. Journal of Fish Diseases 30(1): 13–25.
Bassleer G (1997) Color guide of tropical fish diseases: on freshwater fish. Bassleer Biofish. Westmeerbeek, Belgium. 272p.
Baeverfjord G, Krogdahl A (1996). Development and regression of soybean meal induced enteritis in Atlantic salmon, Salmo salar L., distal intestine: a comparison with the intestines of fasted fish. Journal of Fish Diseases 19: 375–387.
Bento D, Rodrigues RO, Faustino V, Pinho D, Fernandes CS, Pereira AI, Garcia V, Miranda JM, Lima R (2018) Deformation of red blood cells, air bubbles, and droplets in microfluidic devices: flow visualizations and measurements. Micromachines 9: 151. https://doi.org/10.3390/mi9040151
Boggs TE, Friedman JS, Gross JB (2022) Alterations to cavefish red blood cells provide evidence of adaptation to reduced subterranean oxygen. Scientific Reports 12: 3735. https://doi.org/10.1038/s41598-022-07619-0
Borges JCS, Pressinotti LN, Gomes V, Da Silva JRMC (2010) Lipidic and proteic absorption in digestive tract of tropical fat snook (Centropomus parallelus Poey, 1860). Journal of Experimental Marine Biology and Ecology, 386(1–2): 39–44. https://doi.org/10.1016/j.jembe.2010.02.013
Burnham LL, Cabrera MR, Kim IH, Hines RH, Hancock JD (2010) Alcohol- and water-extracted soy protein concentrates for early-weaned pigs. http://hdl.handle.net/2097/3381
Chaparro N, Gaitán S, Acero A (2006) Captura y transporte de reproductores de róbalo Centropomus sp. Revista del Instituto de Investigaciones Tropicales 3: 19–24.
Concha-Frías B, Álvarez-González CA, Gaxiola G, Chiappa X, Sánchez-Zamora A, Martínez-García R, Cruz-Alvarado FJDL (2018) Dietary protein requirement in common snook (Centropomus undecimalis) juveniles reared in marine and brackish water. Ecosistemas y Recursos Agropecuarios 5(13): 45–54. https://doi.org/10.19136/era.a5n13.1393
Corredor-Barrios R (2011) Características morfométricas de las células sanguíneas de cachama blanca (Piaractus brachypomus) alimentadas con suplemento de ensilaje de vísceras de pescado durante la etapa de ceba. CITECSA 2(2): 68–74. https://unipaz.edu.co/revistas/revcitecsa/article/view/14
De Verdal H, Komen H, Quillet E, Chatain B, Allal F, Benzie JA, Vandeputte M (2018) Improving feed efficiency in fish using selective breeding: a review. Reviews in Aquaculture 10(4): 833-851. https://doi.org/10.1111/raq.12202
Esmaeili N (2021) Blood performance: a new formula for fish growth and health. Biology 10, 1236. https://doi.org/10.3390/biology10121236
Fang J, Chen K, Cui HM, Peng X, Li T, Zuo ZC (2014) Morphological and cytochemical studies of peripheral blood cells of Schizothorax prenanti. Anatomia, Histologia, Embryologia 43(5): 386-394. https://doi.org/10.1111/ahe.12089
Fazio F (2019) Fish hematology analysis as an important tool of aquaculture: a review. Aquaculture 500: 237-242. https://doi.org/10.1016/j.aquaculture.2018.10.030
Gaitán-Ibarra S, Benitez-Polo Z, Pulgar-Bacca A, Blanco-Cervantes G, & López-Patiño D. (2024). Effect of growth stimulants on performance indicators and morphoerythrocyte aspects evaluated in juveniles of Centropomus undecimalis (Bloch 1792). Intropica, 19(1): https://doi.org/10.21676/23897864.5980
Gatlin DM III, Barrows FT, Brown P, Dabrowski K, Gaylord TG, Hardy RW, Wurtele E (2007) Expanding the utilization of sustainable plant products in aquafeeds: a review. Aquaculture Research, 38: 551–579.
González-Rodríguez B, Castillo-Vargasmachuca S (2012) The effect of substituting fish meal with soybean meal on growth, feed efficiency, body composition and blood chemistry in juvenile spotted rose snapper Lutjanus guttatus (Steindachner, 1869). Aquaculture 364: 180–185. https://doi.org/10.1016/j.aquaculture.2012.08.007
Hancock JD, Lewis AJ, Peo Jr ER (1989) Effects of ethanol extraction on the utilization of soybean protein by growing rats. Nutrition Reports International 39: 813–821.
Hell R, Hillebrand H (2001) Plant concepts for mineral acquisition and allocation. Current Opinion in Biotechnology 12(2): 161-168.
Hemre GI, Lambertsen G, Lie Ø (1991) The effect of dietary carbohydrate on the stress response in cod (Gadus morhua). Aquaculture 95(3-4): 319-328. https://doi.org/10.1016/0044-8486(91)90097-Q
Hernández MD, Martínez FJ, Jover M, García BG (2007) Effects of partial replacement of fish meal by soybean meal in sharpsnout seabream (Diplodus puntazzo) diet. Aquaculture 263: 159–167. https://doi.org/10.1016/j.aquaculture.2006.07.040
Hekmatpour F, Nazemroaya S, Mousavi SM, Amiri F, Feshalami MY, Sadr AS, Sharifian M (2023) Digestive function and serum biochemical parameters of juvenile Cyprinus carpio in response to substitution of dietary soybean meal with sesame seed (Sesamum indicum) cake. Aquaculture Reports 28: 101438.
Hosseini SA, Khajepour F (2013) Effect of partial replacement of dietary fish meal with soybean meal on some hematological and serum biochemical parameters of juvenile beluga Huso huso. Iranian Journal of Fisheries Sciences 12(2): 348-356. http://jifro.ir/article-1-994-en.html . Fecha de consulta: 3 de octubre de 2025.
Howlader S, Sumi KR, Sarkar S, Billah SM, Ali ML, Howlader J, Shahjahan M (2023) Effects of dietary replacement of fish meal by soybean meal on growth, feed utilization, and health condition of stinging catfish, Heteropneustes fossilis. Saudi Journal of Biological Sciences 30: 103601. https://doi.org/10.1016/j.sjbs.2023.103601
Hussain SM., Bano AA, Ali S, Rizwan M, Adrees M, Zahoor AF, Naeem A (2024) Substitution of fishmeal: Highlights of potential plant protein sources for aquaculture sustainability. Heliyon 10(4). https://doi.org/10.1016/j.heliyon.2024.e26573
Ighwela KA, Ahmad AB, Abol-Munafi AB (2012) Haematological changes in Nile tilapia (Oreochromis niloticus) fed with varying dietary maltose levels. World Journal of Fish and Marine Sciences 4(4): 376-381.
Iqbal M, Yaqub A, Ayub M (2022) Partial and full substitution of fish meal and soybean meal by canola meal in diets for genetically improved farmed tilapia (Oreochromis niloticus): Growth performance, carcass composition, serum biochemistry, immune response, and intestine histology. Journal of Applied Aquaculture 34(4): 829-854.
Jesús-Ramírez F, Álvarez-González CA, Nolasco-Soria HG, Peña E, Martínez-García R, Camarillo-Coop S, Pohlenz C (2017) Caracterización parcial de proteasas digestivas del chucumite (Centropomus parallelus). Hidrobiológica 27(3): 419-427.
Jones DB, Hancock JD, Nelsson JL, Li DF (1989) Effect of replacing milk products with a soybean protein isolate milk replacer in diets for nursery pigs. In: Kansas Swine Day 1989 Proceedings. Kansas State University Agricultural Experiment Station and Cooperative Extension Service. Manhattan, USA. pp. 54–60.
Krishnan HB, Jez JM (2018) The promise and limits for enhancing sulfur-containing amino acid content of soybean seed. Plant Science 272: 14–21. https://doi.org/10.1016/j.plantsci.2018.03.030
Krogdahl A, Bakke-McKellep AM, Baeverfjord G (2003) Effects of graded levels of standard soybean meal on intestinal structure, mucosal enzyme activities, and pancreatic response in Atlantic salmon (Salmo salar L.). Aquaculture Nutrition 9(6): 361-371.
Kumar S, Sahu NP, Pal AK, Choudhury D, Yengkokpam S, Mukherjee SC (2005) Effect of dietary carbohydrate on haematology, respiratory burst activity and histological changes in Labeo rohita juveniles. Fish & shellfish immunology 19(4): 331-344. https://doi.org/10.1016/j.fsi.2005.03.001
Kunitz M (1946) Crystalline soybean trypsin inhibitor II. Journal of General Physiology 30: 291-310. https://doi.org/10.1085/jgp.30.4.291
Lazzari R, Radünz Neto J, Pedron FDA, Loro VL, Pretto A, Gioda CR (2010) Protein sources and digestive enzyme activities in jundiá (Rhamdia quelen). Scientia Agricola 67: 259-266. https://doi.org/10.1590/S0103-90162010000300002
Li P, Liang XF, Luo L, Wang ZY, Wang Y, et al. (2008) Effects of dietary protein levels on growth performance, body composition and liver glutamate dehydrogenase activities of juvenile hybrid tilapia, Oreochromis niloticus × O. aureus. Aquaculture 280(1–4): 242–246. https://doi.org/10.1016/j.aqrep.2020.100572.
Li Y, Ai QH, Mai KS, Xu W, Cheng ZY (2012) Effects of the partial substitution of dietary fish meal by two types of soybean meals on the growth performance of juvenile Japanese seabass, Lateolabrax japonicus (Cuvier, 1828). Aquaculture Research 43: 458–466. https://doi.org/10.1111/j.1365-2109.2011.02849.x
Li Y, Cheng Z, Mai K, Ai Q (2015) Dietary leucine requirement of juvenile Japanese seabass (Lateolabrax japonicus). Journal of Ocean University of China 14: 121–126. https://doi.org/10.1007/s11802-015-2387-5
Liu H, Jin J, Zhu X, Han D, Yang Y, Xie S (2017) Effect of replacement of dietary fish meal by soybean meal on different sizes of gibel carp (Carassius auratus gibelio): digestive enzyme gene expressions and activities, and intestinal and hepatic histology. Aquaculture Nutrition 23: 129–147. https://doi.org/10.1111/anu.12375
Liu X, Han B, Xu J, Zhu J, Hu J, Wan W, Miao S (2020) Replacement of fishmeal with soymeal affects the growth performance, digestive enzymes, intestinal microbiota and immunity of Carassius auratus gibelio♀ × Cyprinus carpio♂. Aquaculture Reports 17: 100472. https://doi.org/10.1016/j.aqrep.2020.100472
Lowry OH, Rosborough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. The Journal of Biological Chemistry 193: 265–275.
Lundstedt LM, Melo JFB, Moraes G (2004) Digestive enzymes and metabolic profile of Pseudoplatystoma corruscans (Teleostei: Siluriformes) in response to diet composition. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 137(3): 331–339. https://doi.org/10.1016/j.cbpc.2003.12.003
Ma Z, Hassan MM, Allais L, He T, Leterme S, Ellis A, Qin JG (2019) Comparison of partial replacement of fishmeal with soybean meal and EnzoMeal on growth performance of Asian seabass Lates calcarifer. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 216: 29–37. https://doi.org/10.1016/j.cbpc.2018.10.006
Megarani DV, Hardian AB, Arifianto D, Santosa CM, Salasia SI (2020) Comparative morphology and morphometry of blood cells in zebrafish (Danio rerio), common carp (Cyprinus carpio carpio), and tilapia (Oreochromis niloticus). Journal of the American Association for Laboratory Animal Science 59(6): 673–680. https://doi.org/10.30802/AALAS-JAALAS-20-000013
Mello GL de, Jerônimo GT, Tancredo KR, Brol J, Almeida EJ de, Martins ML, Tsuzuki MY (2015) Development and health status of Centropomus undecimalis parasitized by Rhabdosynochus rhabdosynochus (Monogenea) under different salinity and temperature conditions. Revista Brasileira de Parasitologia Veterinária 24(3): 350–356. https://doi.org/10.1590/S1984-29612015062
Merrifield D, Olsen RE, Myklebust R, Ringø E (2011) Dietary effect of soybean (Glycine max) products on gut histology and microbiota of fish. In: El-Shemy H (ed) Agricultural and Biological Sciences: Soybean and Nutrition. INTECH Open Access Publisher. London, UK. 231, e50.
Metochis CP, Spanos I, Auchinachie N, Crampton VO, Bell JG, Adams A, Thompson KD (2016) The effects of increasing dietary levels of soy protein concentrate (SPC) on the immune responses and disease resistance (furunculosis) of vaccinated and non-vaccinated Atlantic salmon (Salmo salar L.) parr. Fish & Shellfish Immunology 59: 83–94. Fish & Shellfish Immunology 59, 83-94. https://doi.org/10.1016/j.fsi.2016.10.016
Minasyan HA (2014) Erythrocyte and leukocyte: Two partners in bacteria killing. International Reviews of Immunology 33(6): 490–497. https://doi.org/10.3109/08830185.2014.956359
Misra S, Sahu NP, Pal AK, Xavier B, Kumar S, Mukherjee SC (2006) Pre- and post-challenge immuno-haematological changes in Labeo rohita juveniles fed gelatinised or non-gelatinised carbohydrate with n-3 PUFA. Fish & Shellfish Immunology 21(4): 346-356.
Murashita K, Fukada H, Rønnestad I, Kurokawa T, Masumoto T (2008) Nutrient control of release of pancreatic enzymes in yellowtail (Seriola quinqueradiata): involvement of CCK and PY in the regulatory loop. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 150: 438–443. https://doi.org/10.1016/j.cbpa.2008.05.003
Murashita K, Matsunari H, Furuita H, Rønnestad I, Oku H, Yamamoto T (2018) Effects of dietary soybean meal on the digestive physiology of red seabream Pagrus major. Aquaculture 493: 219–228. https://doi.org/10.1016/j.aquaculture.2018.05.005
Najiah M, Nadirah M, Marina H, Lee SW, Nazaha WH (2008) Quantitative comparisons of erythrocyte morphology in healthy freshwater fish species from Malaysia. Research Journal of Fisheries and Hydrobiology 3(1): 32-35.
Nazir S, Khan N, Fatima M, Azmat H, Naveed S, Ramzan MM, et al. (2023) The influence of dietary protein concentration on digestive enzyme activities, growth, and body composition in juvenile bullseye snakehead (Channa marulius). PLoS ONE 18(2): e0281274. https://doi.org/10.1371/journal.pone.0281274
Nilsson EG, Lofman OC, Block M (1995) Extensive erythrocyte deformation in fish gills observed by in vivo microscopy: apparent means for enhancing oxygen uptake. Journal of Experimental Biology 198: 1150–1156. https://doi.org/10.1242/jeb.198.5.1151
Nunes AJ, Yamamoto H, Simões JP, Pisa JL, Miyamoto N, Leite JS (2023) The black soldier fly (Hermetia illucens) larvae meal can cost-effectively replace fish meal in practical nursery diets for post-larval Penaeus vannamei under high-density culture. Fishes 8(12): 605. https://doi.org/10.3390/fishes8120605
Pearson JP, Brownlee IA (2005) Stomach structure and function. In: Encyclopedia of Human Nutrition. Elsevier. https://doi.org/10.1016/B0-12-226694-3/02263-8
Polonia-Rivera C, Gaitán S, Chaparro-Muñoz N, Villamizar N (2017a) Captura, transporte y aclimatación de juveniles y adultos de róbalo Centropomus undecimalis (Bloch, 1792). Revista Intropica 12: 61–64. https://doi.org/10.21897/rmvz.1133
Polonía C, Gaitán S, Chaparro-Muñoz N, Villamizar N (2017b) Effect of three diets in the experimental culture of the common snook (Centropomus undecimalis Bloch, 1792). Revista MVZ Córdoba 22(3): 6287–6295. https://doi.org/10.21897/rmvz.1133
Rahman M, Baek HJ (2019) Evaluation of erythrocyte morphometric indices in juvenile red spotted grouper, Epinephelus akaara, under elevated water temperature. Development & Reproduction 23(4): 345–353. https://doi.org/10.12717/DR.2019.23.4.345
Rahmah S, Aliyu-Paiko M, Hashim R (2016) In vivo and in vitro protein digestibility in juvenile bagrid catfish Mystus nemurus (Cuvier and Valenciennes, 1840) fed soybean meal-based diets. Aquaculture Research 47: 1392–1401. https://doi.org/10.1111/are.12595
Ranzani-Paiva M J T, Tavares-Dias M (2024) Fish hematology in Brazil: a review. Boletim do Instituto de Pesca 50: e914. https://doi.org/10.20950/1678-2305/bip.2024.50.e914
Rombenso A, Crouse C, Trushenski J (2013) Comparison of traditional and fermented soybean meals as alternatives to fish meal in hybrid striped bass feeds. North American Journal of Aquaculture 75: 197–204. https://doi.org/10.1080/15222055.2012.756440
Ridwanudin A, Sheen SS (2014) Evaluation of dietary fish silage combined with poultry by-product meal or soybean meal to replace fish meal for orange-spotted grouper Epinephelus coioides. Journal of Fish Society of Taiwan 41(4): 287–297.
Rhody NR, Puchulutegui C, Taggart JB, Main KL, Migaud H (2014) Parental contribution and spawning performance in captive common snook Centropomus undecimalis broodstock. Aquaculture 432: 144–153. https://doi.org/10.1016/j.aquaculture.2014.04.022
Santos AA, Egami MI, Ranzani-Paiva MJT, Juliano Y (2009) Hematological parameters and phagocytic activity in fat snook (Centropomus parallelus): seasonal variation, sex and gonadal maturation. Aquaculture 296(3–4): 359–366. https://doi.org/10.1016/j.aquaculture.2009.08.023
Saravanan M, Kumar KP, Ramesh M (2011) Haematological and biochemical responses of freshwater teleost fish Cyprinus carpio (Actinopterygii: Cypriniformes) during acute and chronic sublethal exposure to lindane. Pesticide Biochemistry and Physiology 100(3): 206–211.
Schneider CA, Rasband WS, Eliceiri KW (2012) NIH Image to ImageJ: 25 years of image analysis. Nature Methods 9(7): 671–675. https://doi.org/10.1038/nmeth.2089
Sevinç M, Uğurtaş İH, Yildirimhan HS (2000) Erythrocyte measurements in Lacerta rudis (Reptilia, Lacertidae). Turkish Journal of Zoology 24(2): 207–210.
Silvão CF, Nunes AJP (2017) Effect of dietary amino acid composition from proteins alternative to fishmeal on the growth of juveniles of the common snook, Centropomus undecimalis. Revista Brasileira de Zootecnia 46: 569–575. https://doi.org/10.1590/S1806-92902017000700003
Southamani C, Shanthi G, Deivasigamani M (2015) Hematological response in three Indian major carps in relation to supplementary feeding. International Journal of Fisheries and Aquatic Studies 3(2): 287–294.
Srivastava S, Choudhary SK (2010) Effect of artificial photoperiod on the blood cell indices of the catfish, Clarias batrachus. Journal of Stress Physiology and Biochemistry 6: 22–32.
Trejo-Escamilla I, Galaviz MA, Flores-Ibarra M, González CAA, López LM (2017) Replacement of fishmeal by soya protein concentrate in the diets of Totoaba macdonaldi (Gilbert, 1890) juveniles: effect on the growth performance, in vitro digestibility, digestive enzymes and the haematological and biochemistry parameters. Aquaculture Research 48: 4038–4057. https://doi.org/10.1111/are.13225
Vidal-López JM, Álvarez-González CA, Contreras-Sanchez WM, Patiño R, Hernández-Franyutti AA, Hernández-Vidal U (2012) Feminization of young common snook Centropomus undecimalis (Bloch 1792) using 17B-estradiol. Revista Ciencias Marinas y Costeras 4: 83–93. http://revistas.una.ac.cr/index.php/revmar/article/view/4800/4621
Walter HE (1984) Proteinases: methods with hemoglobin, casein and Azocoll as substrates. In: Bergmeyer HU (ed) Methods of Enzymatic Analysis. Verlag Chemie. Weinheim, Germany. pp. 270–277.
Wang Z, Yang L, Zhang C, Li X, Wang L, Lu K, Song K (2021) Effects of substituting dietary fish meal with soybean meal isolate on growth performance, digestive enzyme activity, and intestinal morphology of spotted seabass (Lateolabrax maculatus). The Israeli Journal of Aquaculture 73: 1542451. https://doi.org/10.46989/001c.29920
Witeska M, Kondera E, Bojarski B (2023) Hematological and hematopoietic analysis in fish toxicology: a review. Animals 13: 2625. https://doi.org/10.3390/ani13162625
Yu DH, Gong SY, Yuan YC, Lin YC (2012) Effects of replacing fish meal with soybean meal on growth, body composition and digestive enzyme activities of juvenile Chinese sucker Myxocyprinus asiaticus. Aquaculture Nutrition 19(1): 84–90. https://doi.org/10.1111/j.1365-2095.2012.00945.x
Zhang C, Rahimnejad S, Wang YR, Lu K, Song K, Wang L, Mai K (2018) Substituting fish meal with soybean meal in diets for Japanese seabass (Lateolabrax japonicus): effects on growth, digestive enzyme activity, gut histology, and expression of gut inflammatory and transporter genes. Aquaculture 483: 173–182. https://doi.org/10.1016/j.aquaculture.2017.10.029
Zhou Z, Ringø E, Olsen RE, Song SK (2018) Dietary effects of soybean products on gut microbiota and immunity of aquatic animals: a review. Aquaculture Nutrition 24: 644–665. https://doi.org/10.1111/anu.12532
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