Azospirillum brasilense, Bacillus subtilis and Serendipita indica as a complement in the nutrition of chili
DOI:
https://doi.org/10.19136/era.a13n2.5092Keywords:
bacteria, endophyte fungus, biofertilizer, co-inoculation, Capsicum annuum L.Abstract
Mexico ranks fourth in the world in chili pepper (Capsicum annuum L.) production, which means that this crop is of great economic importance to Mexican society. However, the excessive and/or unbalanced use of chemical fertilizers has caused the degradation of soil properties and, consequently, a decrease in crop yields. One alternative to reducing the use of chemical fertilizers is the use of beneficial microorganisms, which can provide nutrients. Therefore, the objective of the study was to evaluate the effect of Azospirillum brasilense, Bacillus subtilis and Serendipita indica, applied as a complement to chemical fertilization under two cultivation systems (greenhouse and open field) on the growth, quality and yield of the chili crop. The studies were carried out under a factorial arrangement with two factors: the chemical fertilization factor with two levels (70 and 100%), and the microorganisms factor with eight levels (no microorganisms, Azospirillum brasilense + Bacillus subtilis + Serendipita indica, S. indica, A. brasilense, B. subtilis, A. brasilense + S. indica, B. subtilis + S. indica and B. subtilis + A. brasilense). Measurements were made of variables related to the vegetative growth stage and variables related to the quality and yield of the crop during harvest. The combination of A. brasilense and S. indica had a positive effect on the growth, quality, and yield of the chili pepper crop.
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Abdelaziz ME, Abdelsattar M, Abdeldaym EA, Atia MA, Mahmoud AWM, Saad MM, Hirt H (2019) Piriformospora indica alters Na+/K+ homeostasis, antioxidant enzymes and LeNHX1 expression of greenhouse tomato grown under salt stress. Scientia horticulturae 256: e108532. https://doi.org/10.1016/j.scienta.2019.05.059
Adigoun RF, Aholoukpè HN, Durand A, Tchokponhoué DA, Hotègni NVF, Achigan DEG, Benizri E (2026) Leveraging plant-bacteria interactions for sustainable production of perennial fruit crops. Current Plant Biology 46: e100600.
Aguirre-Medina JF, Espinosa-Moreno JA (2016) Crecimiento y rendimiento de Capsicum annuum L. inoculado con endomicorriza y rizobacterias. Revista Mexicana de Ciencias Agrícolas 7(7): 1539-1550. https://doi.org/10.29312/remexca.v7i7.148
Angulo-Castro A, Ferrera-Cerrato R, Alarcón A, Almaraz-Suárez JJ, Delgadillo-Martínez J, Jiménez-Fernández M, García-Barradas O (2021) Improved growth of bell pepper (Capsicum annuum) plants by inoculating arbuscular mycorrhizal fungi and beneficial rhizobacteria. Scientia Fungorum 51: e1299. https://doi.org/10.33885/SF.2021.51.1299
Balyan G, Pandey AK (2024) Root exudates, the warrior of plant life: revolution below the ground. South African Journal of Botany 164: 280-287.
Bernados LC, Espineli JP, Anarna JA, Aggangan NS (2024) Increasing Tomato Productivity through Integrated Nutrient Sources and Inoculation with Arbuscular Mycorrhizal Fungi and Azospirillum spp. Horticulturae 10(10): e1056. https://doi.org/10.3390/HORTICULTURAE10101056
Caulier S, Nannan C, Gillis A, Licciardi F, Bragard C, Mahillon J (2019) Overview of the Antimicrobial Compounds Produced by Members of the Bacillus subtilis Group. Frontiers in Microbiology 10: e00302. https://doi.org/10.3389/fmicb.2019.00302
Chamorro-Anaya LM, Pérez-Cordero A (2020) Bacillus cereus bacteria endófita promotora de crecimiento vegetal. Revista Colombiana de Biotecnología 22(2): 18–23. https://doi.org/10.15446/REV.COLOMB.BIOTE.V22N2.81723
Chauhan P, Sharma N, Tapwal A, Kumar A, Verma GS, Meena M, Seth CS, Swapnil P (2023) Soil microbiome: Diversity, benefits and interactions with plants. Sustainability 15(19): e14643. https://doi.org/10.3390/su151914643
Chaudhary P, Singh S, Chaudhary A, Sharma A, Kumar G (2022) Overview of biofertilizers in crop production and stress management for sustainable agriculture. Frontiers in plant science 13: e930340. https://doi.org/10.3389/fpls.2022.930340
Chen Q, Song Y, An Y, Lu Y, Zhong G (2024) Soil Microorganisms: Their role in enhancing crop nutrition and health. Diversity 16(12): e734. https://doi.org/10.3390/D16120734
del Barrio-Duque A, Ley J, Samad A, Antonielli L, Sessitsch A, Compant S (2019) Beneficial endophytic bacteria-Serendipita indica Interaction for crop enhancement and resistance to phytopathogens. Frontiers in Microbiology 10: e02888. https://doi.org/10.3389/fmicb.2019.02888
Di Rienzo JA, Casanoves F, Balzarini MG, Gonzalez L, Tablada M, Robledo CW InfoStat versión 2020 Centro de transferencia InfoStat, FCA, Universidad Nacional de Córdoba, Argentina. URL http://www.infostat.com.ar. Fecha de consulta: 01 de diciembre de 2025.
Duchen DG, Torres JM (2021) Interacción de bacterias y plantas en la fijación del nitrógeno. Revista de Investigación e Innovación Agropecuaria y de Recursos Naturales 8(2): 87–101. https://doi.org/10.53287/UYXF4027GF99E
El-Egami HM, Hegab RH, Montaser H, El-Hawary MM, Hasanuzzaman M (2024) Impact of potassium-solubilizing microorganisms with potassium sources on the growth, physiology, and productivity of wheat crop under salt-affected soil conditions. Agronomy 14(3): e0423. https://doi.org/10.3390/AGRONOMY14030423
FAOSTAT (2026) Base de datos estadísticos corporativos de la Organización para la Agricultura y la alimentación (FAO). https://www.fao.org/faostat/es/#data/QCL/visualize. Fecha de consulta: 20 de enero del 2026
Gamboa-Angulo J, Ruíz-Sánchez E, Alvarado-López C, Gutiérrez-Miceli F, Ruíz-Valdiviezo VM, Medina-Dzul K (2020) Efecto de biofertilizantes microbianos en las características agronómicas de la planta y calidad del fruto del chile xcat´ik (Capsicum annuum L.). Terra Latinoamericana 38(4): 817-826. https://doi.org/10.28940/TERRA.V38I4.716
Ghorbani A, Razavi SM, Omran VG, Pirdashti H (2018) Piriformospora indica alleviates salinity by boosting redox poise and antioxidative potential of tomato. Russian Journal of Plant Physiology 65(6): 898-907. https://doi.org/10.1134/S1021443718060079
González FH, Fuentes-Molina N (2017) Mecanismo de acción de cinco microorganismos promotores de crecimiento vegetal. Revista de Ciencias Agrícolas 34(1): 17–31. https://doi.org/10.22267/rcia.173401.60
Guan DX, Menezes BD, Li G (2024). The importance of mineral elements for sustainable crop production. Agronomy 14(1): e0209. https://doi.org/10.3390/agronomy14010209
Guan Z, Lin D, Chen D, Guo Y, Lu Y, Han Q, Li N, Su Y, Li J, Wang J, Ma W, Qiu Q, He Q (2022) Soil microbial communities response to different fertilization regimes in young Catalpa bungei plantation. Frontiers in Microbiology 13: e948875. https://doi.org/10.3389/FMICB.2022.948875
Hamzah M, Usman K, Rizwan M, Al Jabri H, Alsafran M (2022) Functions and strategies for enhancing zinc availability in plants for sustainable agriculture. Frontiers in Plant Science 13: e1033092. https://doi.org/10.3389/fpls.2022.1033092
Jacoby R, Peukert M, Succurro A, Koprivova A, Kopriva S (2017) The Role of Soil Microorganisms in Plant Mineral Nutrition-Current Knowledge and Future Directions. Frontiers in Plant Science 8: e01617. https://doi.org/10.3389/FPLS.2017.01617
Jahandideh-Mahjen AVA, Sepehri M, Khatabi B, Rezaei M (2021) Alleviation of zinc deficiency in wheat inoculated with root endophytic fungus Piriformospora indica and rhizobacterium Pseudomonas putida. Rhizosphere 17: e100311. https://doi.org/10.1016/J.RHISPH.2021.100311
Ji-Hao ZM, Huang C, Lin W, Lu Y, Wang P, Dong B, He B, Wu B, Guo L (2024) The impact of Piriformospora indica on plant heat and drought tolerance. Frontiers in Plant Science 15: e1479561. https://doi.org/10.3389/FPLS.2024.1479561
Kolesnikov Y, Kretyni S, Markhaichuk V, Filepov R, Dobrev PI, Martinec J, Kravets V (2025) The role of zinc in regulation of plant metabolism: What is known to date?. Journal of Plant Growth Regulation 45: 1639-1660. https://doi.org/10.1007/s00344-025-11975-2
Krasilnikov P, Taboada MA, Amanullah (2022) Fertilizer use, soil health and agricultural sustainability. Agriculture 12(4): e0462. https://doi.org/10.3390/agriculture12040462
Kudirka G, Viršilė A, Sutulienė R, Laužikė K, Samuolienė G (2023) Precise management of hydroponic nutrient solution pH: The effects of minor pH changes and MES buffer molarity on lettuce physiological properties. Horticulturae 9(7): e0837. https://doi.org/10.3390/HORTICULTURAE9070837
Liang S, Yingning ZOU, Bo SHU, Qiangsheng WU (2024) Arbuscular mycorrhizal fungi and endophytic fungi differentially modulate polyamines or proline of peach in response to soil flooding. Pedosphere 34(2): 460-472. https://doi.org/10.1016/j.pedsph.2023.05.002
Li H, Fu S, Zhu J, Gao W, Chen L, Li X, Zhang S, Zheng S, Zhang H, Liu Y (2022) Nitric oxide generated by Piriformospora indica-induced nitrate reductase promotes tobacco growth by regulating root architecture and ammonium and nitrate transporter gene expression. Journal of Plant Interactions 17(1): 861-872. https://doi.org/10.1080/17429145.2022.2108926
Li L, Feng Y, Qi F, Hao R (2023) Research Progress of Piriformospora indica in Improving Plant Growth and Stress Resistance to Plant. Journal of Fungi 9(10): e0965. https://doi.org/10.3390/JOF9100965
Li L, Guo N, Feng Y, Duan M, Li C (2022) Effect of Piriformospora indica-induced systemic resistance and basal Immunity Against Rhizoctonia cerealis and Fusarium graminearum in wheat. Frontiers in Plant Science 13: e836940. https://doi.org/10.3389/FPLS.2022.836940
Li L, Xu M, Ali ME, Zhang W, Duan Y, Li D (2018) Factors affecting soil microbial biomass and functional diversity with the application of organic amendments in three contrasting cropland soils during a field experiment. PLoS ONE 13: e0203812. https://doi.org/10.1371/JOURNAL.PONE.0203812
Li L, Zhu P, Wang X, Zhang Z (2020) Phytoremediation effect of Medicago sativa colonized by Piriformospora indica in the phenanthrene and cadmium co-contaminated soil. BMC biotechnology 20(1): e0020. https://doi.org/10.1186/s12896-020-00613-2
Li M, Watanabe S, Gao F, Dubos C (2023) Iron nutrition in plants: Towards a new paradigm? Plants 12(2): e0384. https://doi.org/10.3390/plants12020384
Lopes MJ dos S, Dias-Filho MB, Gurgel ESC (2021) Successful plant growth-promoting microbes: Inoculation methods and abiotic factors. Frontiers in Sustainable Food Systems 5: e606454. https://doi.org/10.3389/FSUFS.2021.606454
Marastoni L, Pii Y, Maver M, Valentinuzzi F, Cesco S, Mimmo T (2019) Role of Azospirillum brasilense in triggering different Fe chelate reductase enzymes in cucumber plants subjected to both nutrient deficiency and toxicity. Plant Physiology and Biochemistry 136: 118-126. https://doi.org/10.1016/j.plaphy.2019.01.013
Martins S, Brito C, Baltazar M, Dinis LT, Pereira S (2026). Exploring the role of root exudates in shaping plant–soil–microbe interactions to support agroecosystem resilience. Horticulturae 12(1): e0090. https://doi.org/10.3390/horticulturae12010090
Mejía-Bautista MÁ, Cristóbal-Alejo J, Pacheco-Aguilar JR, Reyes-Ramírez A (2022) Bacillus spp. en el crecimiento y rendimiento de Capsicum chinense Jacq. Revista Mexicana de Ciencias Agrícolas 13(1): 115-126. https://doi.org/10.29312/REMEXCA.V13I1.2664
Muhammad A, Kong X, Zheng S, Bai N, Li L, Khan MHU, Fiaz S and Zhang Z (2024) Exploring plant-microbe interactions in adapting to abiotic stress under climate change: a review. Frontiers in Plant Science 15: e1482739. https://doi.org/10.3389/fpls.2024.1482739
Musazade E, Mrisho II, Feng X (2025) Auxin metabolism and signaling: Integrating independent mechanisms and crosstalk in plant abiotic stress responses. Plant Stress 18: e101034. https://doi.org/10.1016/j.stress.2025.101034
Pantigoso HA, Newberger D, Vivanco JM (2022) The rhizosphere microbiome: Plant-microbial interactions for resource acquisition. Journal of Applied Microbiology 133: 2864-2876. https://doi.org/10.1111/jam.15686
Pan L, Cai B (2023) Phosphate-solubilizing bacteria: Advances in their physiology, molecular mechanisms and microbial community effects. Microorganisms 11(12): e2904. https://doi.org/10.3390/microorganisms11122904
Pelagio-Flores R, Ravelo-Ortega G, García-Pineda E, López-Bucio J (2025). A century of Azospirillum: plant growth promotion and agricultural promise. Plant Signaling & Behavior 20(1): e2551609. https://doi.org/10.1080/15592324.2025.2551609
Pérez-Pérez R, Hernández-Forte I, Sanabria-Álvarez YO, Salcedo-Benítez, JC, Sosa-del Castillo D, Pérez-Martínez S (2021) Characterization of potassium solubilizing bacteria isolated from corn rhizoplane. Agronomía Colombiana 39(3): 415–425. https://doi.org/10.15446/AGRON.COLOMB.V39N3.98522
Ritter G, de Vargas RJ, Farinelli D, Cinosi N, Traini C, Facchin SL, Hiromi Kiahara L, da Silva DF, Portarena S, Villa F (2025) Application of Azospirillum brasilense and humic substances improves the nursery quality of olive seedlings in pots. Horticulturae 11(1): 48. https://doi.org/10.3390/HORTICULTURAE11010048
Rodríguez-Hernández MG, Gallegos-Robles MÁ, Rodríguez-Sifuentes L, Fortis-Hernández M, Luna-Ortega GJ, González-Salas U (2020) Native Bacillus spp. Strains as sustainable alternative in the yield of corn forage. Terra Latinoamericana 38(2): 323-331. https://doi.org/10.28940/TERRA.V38I2.690
Rokni N, Shams-Alizadeh H, Bazgir E, Darvishnia M, Mirzaei-Najaofghli H (2021) The tripartite consortium of Serendipita indica, Trichoderma simmonsii, and bell pepper (Capsicum annuum). Biological Control 158: e104608. https://doi.org/10.1016/J.BIOCONTROL.2021.104608
Saleem S, Sekara A, Pokluda R (2022) Serendipita indica - A review from agricultural point of view. Plants 11(24): e3417. https://doi.org/10.3390/PLANTS11243417
Santoyo G (2022) How plants recruit their microbiome? New insights into beneficial interactions. Journal of advanced research 40: 45-58.
SIAP (2026) Anuario estadístico de la producción agrícola en México 2024. Secretaria de Agricultura y Desarrollo Rural. https://nube.siap.gob.mx/cierreagricola/. Fecha de consulta: 20 de enero del 2026.
Silva LI da, Pereira MC, Carvalho AMX de, Buttrós VH, Pasqual M, Dória J (2023) Phosphorus-solubilizing microorganisms: A key to sustainable agriculture. Agriculture 13(2): e0462. https://doi.org/10.3390/AGRICULTURE13020462
Sun W, Shahrajabian MH, Wang N (2025) A study of the different strains of the genus Azospirillum spp. on increasing productivity and stress resilience in plants. Plants 14(2): e0267. https://doi.org/10.3390/PLANTS14020267
Timofeeva AM, Galyamova MR, Sedykh SE (2023) Plant growth-promoting soil bacteria: Nitrogen fixation, phosphate solubilization, siderophore production, and other biological activities. Plants 12(24): e4074. https://doi.org/10.3390/PLANTS12244074
Wang Q, Li S, Li J, Huang D (2024) The utilization and roles of nitrogen in plants. Forests 15(7): e1191. https://doi.org/10.3390/f15071191
Xu L, Wang A, Wang J, Wei Q, Zhang W (2017) Piriformospora indica confers drought tolerance on Zea mays L. through enhancement of antioxidant activity and expression of drought-related genes. The Crop Journal 5(3): 251-258. https://doi.org/10.1016/j.cj.2016.10.002
Youssef SM, Shaaban A, Abdelkhalik A, Abd El Tawwab AR, Abd Al Halim LR, Rabee LA, Alwutayd KM, Ahmed RMM, Alwutayd R, Hemida KA (2023) Compost and phosphorus/potassium-solubilizing fungus effectively boosted quinoa’s physio-biochemical traits, nutrient acquisition, soil microbial community, and yield and quality in normal and calcareous soils. Plants 12(17): e3071. https://doi.org/10.3390/PLANTS12173071
Zeng Q, Hu HW, Ge AH, Xiong C, Zhai CC, Duan GL, Zhang LM (2025) Plant–microbiome interactions and their impacts on plant adaptation to climate change. Journal of Integrative Plant Biology 67(3): 826-844. https://doi.org/10.1111/jipb.13863
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